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Clinical Oral Investigations

Published in:

01-05-2017 | Original Article

Double dye technique and fluid filtration test to evaluate early sealing ability of an endodontic sealer

Authors: Luigi Generali, C. Prati, C. Pirani, F. Cavani, M. R. Gatto, M. G. Gandolfi

Published in: Clinical Oral Investigations | Issue 4/2017

Abstract

Objectives

The aim of the present study is to evaluate the possible correlation between sealer penetration into dentinal tubules and sealing ability both in presence and absence of smear layer.

Materials and methods

Fourteen maxillary central incisors were treated with 5.25 % NaOCl +10 % EDTA to remove the smear layer (SL-free group) or 5.25 % NaOCl without EDTA (SL group). Root canals were filled using #25 Thermafil Obturators with Topseal sealer labelled with 0.1 wt% rhodamine B. Sealing ability was measured as fluid filtration rate with a fluid-flow meter using water supplemented with 0.3 % calcein fluorescent dye. Specimens were sectioned, observed under confocal microscope to co-localize the presence of sealer (rhodamine B labelling) into dentinal tubules and gaps (calcein labelling) into the root canal. The depth of sealer penetration into dentinal tubules and the percentage of sealer penetration around the root canal were measured at 3, 5 and 8 mm from the apex.

Results

No significant differences between groups were observed in fluid filtration rate nor in depth of calcein penetration. Sealer penetration depth and percentage into dentinal tubules were not significantly different between groups, except at 8-mm level in absence of smear layer.

Conclusion

Sealer penetration at 3- and 5-mm levels was not influenced by smear layer while it was significantly reduced at 8-mm level. Fluid filtration rate was not correlated either with depth of calcein penetration nor with sealer penetration into dentinal tubules.

Clinical relevance

The sealing ability of Topseal sealer is not affected by presence or absence of smear layer.

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Title: Double dye technique and fluid filtration test to evaluate early sealing ability of an endodontic sealer
Authors: Luigi Generali
C. Prati
C. Pirani
F. Cavani
M. R. Gatto
M. G. Gandolfi
Publication date: 01-05-2017
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 4/2017
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-016-1878-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Double dye technique and fluid filtration test to evaluate early sealing ability of an endodontic sealer

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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