Skip to main content
Top
Published in: Clinical Oral Investigations 4/2017

01-05-2017 | Original Article

Double dye technique and fluid filtration test to evaluate early sealing ability of an endodontic sealer

Authors: Luigi Generali, C. Prati, C. Pirani, F. Cavani, M. R. Gatto, M. G. Gandolfi

Published in: Clinical Oral Investigations | Issue 4/2017

Login to get access

Abstract

Objectives

The aim of the present study is to evaluate the possible correlation between sealer penetration into dentinal tubules and sealing ability both in presence and absence of smear layer.

Materials and methods

Fourteen maxillary central incisors were treated with 5.25 % NaOCl +10 % EDTA to remove the smear layer (SL-free group) or 5.25 % NaOCl without EDTA (SL group). Root canals were filled using #25 Thermafil Obturators with Topseal sealer labelled with 0.1 wt% rhodamine B. Sealing ability was measured as fluid filtration rate with a fluid-flow meter using water supplemented with 0.3 % calcein fluorescent dye. Specimens were sectioned, observed under confocal microscope to co-localize the presence of sealer (rhodamine B labelling) into dentinal tubules and gaps (calcein labelling) into the root canal. The depth of sealer penetration into dentinal tubules and the percentage of sealer penetration around the root canal were measured at 3, 5 and 8 mm from the apex.

Results

No significant differences between groups were observed in fluid filtration rate nor in depth of calcein penetration. Sealer penetration depth and percentage into dentinal tubules were not significantly different between groups, except at 8-mm level in absence of smear layer.

Conclusion

Sealer penetration at 3- and 5-mm levels was not influenced by smear layer while it was significantly reduced at 8-mm level. Fluid filtration rate was not correlated either with depth of calcein penetration nor with sealer penetration into dentinal tubules.

Clinical relevance

The sealing ability of Topseal sealer is not affected by presence or absence of smear layer.
Literature
1.
go back to reference Mamootil K, Messer HH (2007) Penetration of dentinal tubules by endodontic sealer cements in extracted teeth and in vivo. Int Endod J 40:873–881CrossRefPubMed Mamootil K, Messer HH (2007) Penetration of dentinal tubules by endodontic sealer cements in extracted teeth and in vivo. Int Endod J 40:873–881CrossRefPubMed
2.
go back to reference Torabinejad M, Ung B, Kettering JD (1990) In vitro bacterial penetration of coronally unsealed endodontically treated teeth. J Endod 16:566–569CrossRefPubMed Torabinejad M, Ung B, Kettering JD (1990) In vitro bacterial penetration of coronally unsealed endodontically treated teeth. J Endod 16:566–569CrossRefPubMed
3.
go back to reference Trope M, Chow E, Nissan R (1995) In vitro endotoxin penetration of coronally unsealed endodontically treated teeth. Endod Dent Traumatol 11:90–94CrossRefPubMed Trope M, Chow E, Nissan R (1995) In vitro endotoxin penetration of coronally unsealed endodontically treated teeth. Endod Dent Traumatol 11:90–94CrossRefPubMed
4.
go back to reference Ardizzoni A, Generali L, Righi E, et al. (2014) Differential efficacy of endodontic obturation procedures: an ex vivo study. Odontology 102:223–231CrossRefPubMed Ardizzoni A, Generali L, Righi E, et al. (2014) Differential efficacy of endodontic obturation procedures: an ex vivo study. Odontology 102:223–231CrossRefPubMed
5.
go back to reference Gandolfi MG, Parrilli AP, Fini M, Prati C, Dummer PM (2013) 3D micro-CT analysis of the interface voids associated with Thermafil root fillings used with AH Plus or a flowable MTA sealer. Int Endod J 46:253–263CrossRefPubMed Gandolfi MG, Parrilli AP, Fini M, Prati C, Dummer PM (2013) 3D micro-CT analysis of the interface voids associated with Thermafil root fillings used with AH Plus or a flowable MTA sealer. Int Endod J 46:253–263CrossRefPubMed
6.
go back to reference Foschi F, Nucci C, Montebugnoli L, et al. (2004) SEM evaluation of canal wall dentine following use of Mtwo and ProTaper NiTi rotary instruments. Int Endod J 37:832–839CrossRefPubMed Foschi F, Nucci C, Montebugnoli L, et al. (2004) SEM evaluation of canal wall dentine following use of Mtwo and ProTaper NiTi rotary instruments. Int Endod J 37:832–839CrossRefPubMed
7.
go back to reference Prati C, Foschi F, Nucci C, Montebugnoli L, Marchionni S (2004) Appearance of the root canal walls after preparation with NiTi rotary instruments: a comparative SEM investigation. Clin Oral Invest 8:102–110CrossRef Prati C, Foschi F, Nucci C, Montebugnoli L, Marchionni S (2004) Appearance of the root canal walls after preparation with NiTi rotary instruments: a comparative SEM investigation. Clin Oral Invest 8:102–110CrossRef
8.
go back to reference Gharib SR, Tordik PA, Imamura GM, Baginski TA, Goodell GG (2007) A confocal laser scanning microscope investigation of the Ephiphany obturation system. J Endod 33:957–961CrossRefPubMed Gharib SR, Tordik PA, Imamura GM, Baginski TA, Goodell GG (2007) A confocal laser scanning microscope investigation of the Ephiphany obturation system. J Endod 33:957–961CrossRefPubMed
9.
go back to reference Balguerie E, van der Sluis L, Vallaeys K, Gurgel-Georgelin M, Diemer F (2011) Sealer penetration and adaptation in the dentinal tubules: a scanning electron microscopic study. J Endod 37:1576–1579CrossRefPubMed Balguerie E, van der Sluis L, Vallaeys K, Gurgel-Georgelin M, Diemer F (2011) Sealer penetration and adaptation in the dentinal tubules: a scanning electron microscopic study. J Endod 37:1576–1579CrossRefPubMed
10.
go back to reference Kara Tuncer A, Tuncer S (2012) Effect of different final irrigation solutions on dentinal tubule penetration depth and percentage of root canal sealer. J Endod 38:860–863CrossRefPubMed Kara Tuncer A, Tuncer S (2012) Effect of different final irrigation solutions on dentinal tubule penetration depth and percentage of root canal sealer. J Endod 38:860–863CrossRefPubMed
11.
go back to reference Bolles JA, He J, Svoboda KKH, Schneiderman E, Glickman GN (2013) Comparison of vibringe, endoactivator and needle irrigation on sealer penetration in extracted human teeth. J Endod 39:708–711CrossRefPubMed Bolles JA, He J, Svoboda KKH, Schneiderman E, Glickman GN (2013) Comparison of vibringe, endoactivator and needle irrigation on sealer penetration in extracted human teeth. J Endod 39:708–711CrossRefPubMed
12.
go back to reference Kuçj A, Alaçam T, Yavaş O, Ergul-Ulger Z, Kayaoglu G (2014) Sealer penetration into dentinal tubules in the presence or absence of smear layer: a confocal laser scanning microscopic study. J Endod 40:1627–1631CrossRef Kuçj A, Alaçam T, Yavaş O, Ergul-Ulger Z, Kayaoglu G (2014) Sealer penetration into dentinal tubules in the presence or absence of smear layer: a confocal laser scanning microscopic study. J Endod 40:1627–1631CrossRef
13.
go back to reference White RR, Goldman M, Lin PS (1984) The influence of the smear layer upon dentinal tubule penetration by plastic filling materials. J Endod 10:558–562CrossRefPubMed White RR, Goldman M, Lin PS (1984) The influence of the smear layer upon dentinal tubule penetration by plastic filling materials. J Endod 10:558–562CrossRefPubMed
14.
go back to reference Çalt S, Serper A (1999) Dentinal tubule penetration of root canal sealers after root canal dressing with calcium hydroxide. J Endod 25:431–433CrossRefPubMed Çalt S, Serper A (1999) Dentinal tubule penetration of root canal sealers after root canal dressing with calcium hydroxide. J Endod 25:431–433CrossRefPubMed
15.
go back to reference Patel DV, Sherriff M, Ford TR, et al. (2007) The penetration of RealSeal primer and Tubliseal into root canal dentinal tubules: a confocal microscopic study. Int Endod J 40:67–71CrossRefPubMed Patel DV, Sherriff M, Ford TR, et al. (2007) The penetration of RealSeal primer and Tubliseal into root canal dentinal tubules: a confocal microscopic study. Int Endod J 40:67–71CrossRefPubMed
16.
go back to reference Ordinola-Zapata R, Bramante CM, Bernardineli N, et al. (2009) A preliminary study of the percentage of sealer penetration in roots obturated with the Thermafil and RealSeal-1 obturation techniques in mesial root canals of mandibular molars. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:961–968 Ordinola-Zapata R, Bramante CM, Bernardineli N, et al. (2009) A preliminary study of the percentage of sealer penetration in roots obturated with the Thermafil and RealSeal-1 obturation techniques in mesial root canals of mandibular molars. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:961–968
17.
go back to reference Şen BH, Pişkin B, Baran N (1996) The effect of tubular penetration of root canal sealers on dye microleakage. Int Endod J 29:23–28CrossRefPubMed Şen BH, Pişkin B, Baran N (1996) The effect of tubular penetration of root canal sealers on dye microleakage. Int Endod J 29:23–28CrossRefPubMed
18.
go back to reference Engel GT, Goodell GG, McClanahan SB (2005) Sealer penetration and apical microleakage in smear-free dentin after a final rinse with either 70 % isopropyl alcohol or Peridex. J Endod 31:620–623CrossRefPubMed Engel GT, Goodell GG, McClanahan SB (2005) Sealer penetration and apical microleakage in smear-free dentin after a final rinse with either 70 % isopropyl alcohol or Peridex. J Endod 31:620–623CrossRefPubMed
19.
go back to reference Stevens RW, Strother JM, Mcclanahan SB (2006) Leakage and sealer penetration in smear-free dentin after a final rinse with 95 % ethanol. J Endod 32:785–788CrossRefPubMed Stevens RW, Strother JM, Mcclanahan SB (2006) Leakage and sealer penetration in smear-free dentin after a final rinse with 95 % ethanol. J Endod 32:785–788CrossRefPubMed
20.
go back to reference De Deus G, Brandão MC, Leal F, et al. (2012) Lack of correlation between sealer penetration into dentinal tubules and sealability in nonbonded root fillings. Int Endod J 45:642–651CrossRefPubMed De Deus G, Brandão MC, Leal F, et al. (2012) Lack of correlation between sealer penetration into dentinal tubules and sealability in nonbonded root fillings. Int Endod J 45:642–651CrossRefPubMed
21.
go back to reference Machado R, Silva Neto UX, Cameiro E, Fariniuk LF, Westphalen VPD, Cunha RS (2014) Lack of correlation between tubular dentine cement penetration, adhesiveness and leakage in roots filled with gutta percha and an endodontic cement based on epoxy amine resin. J Appl Oral Sci 22:22–28CrossRefPubMedPubMedCentral Machado R, Silva Neto UX, Cameiro E, Fariniuk LF, Westphalen VPD, Cunha RS (2014) Lack of correlation between tubular dentine cement penetration, adhesiveness and leakage in roots filled with gutta percha and an endodontic cement based on epoxy amine resin. J Appl Oral Sci 22:22–28CrossRefPubMedPubMedCentral
22.
go back to reference McComb D, Smith DC (1975) A preliminary scanning electron microscopic study of root canals after endodontic procedures. J Endod 1:238–242CrossRefPubMed McComb D, Smith DC (1975) A preliminary scanning electron microscopic study of root canals after endodontic procedures. J Endod 1:238–242CrossRefPubMed
23.
go back to reference Cergneux M, Ciucchi B, Dietschi JM, Holz J (1987) The influence of the smear layer on the sealing ability of canal obturation. Int Endod J 20:228–232CrossRefPubMed Cergneux M, Ciucchi B, Dietschi JM, Holz J (1987) The influence of the smear layer on the sealing ability of canal obturation. Int Endod J 20:228–232CrossRefPubMed
24.
go back to reference Shahravan A, Haghdoost AA, Adl A, Rahimi H, Shadifar F (2007) Effect of smear layer on sealing ability of canal obturation: a systematic review and meta-analysis. J Endod 33:96–105CrossRefPubMed Shahravan A, Haghdoost AA, Adl A, Rahimi H, Shadifar F (2007) Effect of smear layer on sealing ability of canal obturation: a systematic review and meta-analysis. J Endod 33:96–105CrossRefPubMed
25.
go back to reference Şen BH, Wesselink PR, Türkün M (1995) The smear layer: a phenomenon in root canal therapy. Int Endod J 28:141–148CrossRefPubMed Şen BH, Wesselink PR, Türkün M (1995) The smear layer: a phenomenon in root canal therapy. Int Endod J 28:141–148CrossRefPubMed
26.
27.
go back to reference Saleh IM, Ruyter IE, Haapasalo M, Ørstavik D (2002) The effects of dentine pretreatment on the adhesion of root-canal sealers. Int Endod J 35:859–866CrossRefPubMed Saleh IM, Ruyter IE, Haapasalo M, Ørstavik D (2002) The effects of dentine pretreatment on the adhesion of root-canal sealers. Int Endod J 35:859–866CrossRefPubMed
28.
go back to reference Michelich VJ, Schuster GS, Pashley DH (1980) Bacterial penetration of human dentin in vitro. J Dent Res 59:1398–1403CrossRefPubMed Michelich VJ, Schuster GS, Pashley DH (1980) Bacterial penetration of human dentin in vitro. J Dent Res 59:1398–1403CrossRefPubMed
29.
go back to reference Torabinejad M, Handysides R, Khademi AA, Bakland LK (2002) Clinical implications of the smear layer in endodontics: a review. Oral Surg Oral Med Oral Phatol Oral Radiol Endod 94:658–666CrossRef Torabinejad M, Handysides R, Khademi AA, Bakland LK (2002) Clinical implications of the smear layer in endodontics: a review. Oral Surg Oral Med Oral Phatol Oral Radiol Endod 94:658–666CrossRef
30.
go back to reference Generali L, Righi E, Todesca MV, Consolo U (2014) Canal shaping with WaveOne reciprocating files: influence of operator experience on instrument breakage and canal preparation time. Odontology 102:217–222CrossRefPubMed Generali L, Righi E, Todesca MV, Consolo U (2014) Canal shaping with WaveOne reciprocating files: influence of operator experience on instrument breakage and canal preparation time. Odontology 102:217–222CrossRefPubMed
31.
go back to reference Gandolfi MG, Prati C (2010) MTA and F-doped MTA cements used as sealers with warm gutta-percha. Long-term study of sealing ability. Int Endod J 43:889–901CrossRefPubMed Gandolfi MG, Prati C (2010) MTA and F-doped MTA cements used as sealers with warm gutta-percha. Long-term study of sealing ability. Int Endod J 43:889–901CrossRefPubMed
32.
go back to reference Iacono F, Pedullà E, Rapisarda E, Prati C, Gandolfi MG (2014) Long term stability of different obturation systems after 12 months in simulated body fluids. Gen Dent 62:20–23PubMed Iacono F, Pedullà E, Rapisarda E, Prati C, Gandolfi MG (2014) Long term stability of different obturation systems after 12 months in simulated body fluids. Gen Dent 62:20–23PubMed
33.
go back to reference Camilleri J, Gandolfi MG, Siboni F, Prati C (2011) Dynamic sealing ability of MTA root canal sealer. Int Endod J 44:9–20CrossRefPubMed Camilleri J, Gandolfi MG, Siboni F, Prati C (2011) Dynamic sealing ability of MTA root canal sealer. Int Endod J 44:9–20CrossRefPubMed
34.
go back to reference Bitter K, Paris S, Martus P, Schartner R, Kielbassa AM (2004) A confocal laser scanning microscope investigation of different dental adhesives bonded to root canal dentine. Int Endod J 37:840–848CrossRefPubMed Bitter K, Paris S, Martus P, Schartner R, Kielbassa AM (2004) A confocal laser scanning microscope investigation of different dental adhesives bonded to root canal dentine. Int Endod J 37:840–848CrossRefPubMed
35.
go back to reference Weis MV, Parashos P, Messer HH (2004) Effect of obturation technique on sealer cement thickness and dentinal tubule penetration. Int Endod J 37:653–663CrossRefPubMed Weis MV, Parashos P, Messer HH (2004) Effect of obturation technique on sealer cement thickness and dentinal tubule penetration. Int Endod J 37:653–663CrossRefPubMed
36.
go back to reference Kok D, Húngaro Duarte MA, Abreu Da Rosa R, Wagner MH, Pereira JR, Só MV (2012) Evaluation of epoxy resin sealer after three root canal filling technique by confocal laser scanning microscopy. Microsc Res Tech 75:1277–1280CrossRefPubMed Kok D, Húngaro Duarte MA, Abreu Da Rosa R, Wagner MH, Pereira JR, Só MV (2012) Evaluation of epoxy resin sealer after three root canal filling technique by confocal laser scanning microscopy. Microsc Res Tech 75:1277–1280CrossRefPubMed
37.
go back to reference D’Alpino PH, Pereira JC, Svizero NR, Rueggeberg FA, Pashley DH (2006) Use of fluorescent compounds in assessing bonded resin-based restorations: a literature review. J Dent 34:623–634CrossRefPubMed D’Alpino PH, Pereira JC, Svizero NR, Rueggeberg FA, Pashley DH (2006) Use of fluorescent compounds in assessing bonded resin-based restorations: a literature review. J Dent 34:623–634CrossRefPubMed
38.
go back to reference Watson TF (1989) A confocal optical microscope study of the morphology of the tooth/restoration interface using Scotchbond 2 dentin adhesive. J Dent Res 68:1124–1131CrossRefPubMed Watson TF (1989) A confocal optical microscope study of the morphology of the tooth/restoration interface using Scotchbond 2 dentin adhesive. J Dent Res 68:1124–1131CrossRefPubMed
39.
go back to reference Griffiths BM, Watson TF (1995) Resin–dentin interface of scotchbond multi-purpose dentin adhesive. Am J Dent 8:212–216PubMed Griffiths BM, Watson TF (1995) Resin–dentin interface of scotchbond multi-purpose dentin adhesive. Am J Dent 8:212–216PubMed
40.
go back to reference Hilton TJ (2002) Can modern restorative procedures and materials reliably seal cavities? In vitro investigations. Part 1. Am J Dent 15:198–210PubMed Hilton TJ (2002) Can modern restorative procedures and materials reliably seal cavities? In vitro investigations. Part 1. Am J Dent 15:198–210PubMed
42.
go back to reference Oksan T, Aktener BO, Sen BH, Tezel H (1993) The penetration of root canal sealers into dentinal tubules. A scanning electron microscopic study. Int Endod J 26:301–305CrossRefPubMed Oksan T, Aktener BO, Sen BH, Tezel H (1993) The penetration of root canal sealers into dentinal tubules. A scanning electron microscopic study. Int Endod J 26:301–305CrossRefPubMed
43.
go back to reference Kokkas AB, Boutsioukis AC, Vassiliadis LP, Stavrianos CK (2004) The influence of the smear layer on dentinal tubule penetration depth by three different root canal sealers: an in vitro study. J Endod 30:100–102CrossRefPubMed Kokkas AB, Boutsioukis AC, Vassiliadis LP, Stavrianos CK (2004) The influence of the smear layer on dentinal tubule penetration depth by three different root canal sealers: an in vitro study. J Endod 30:100–102CrossRefPubMed
44.
go back to reference Zhou HM, Shen Y, Zheng W, Li L, Zheng YF, Haapasalo M (2013) Physical properties of 5 root canal sealers. J Endod 39:1281–1286CrossRefPubMed Zhou HM, Shen Y, Zheng W, Li L, Zheng YF, Haapasalo M (2013) Physical properties of 5 root canal sealers. J Endod 39:1281–1286CrossRefPubMed
45.
go back to reference Vassiliadis LP, Sklavounos SA, Stavrianos CK (1994) Depth of penetration and appearance of Grossman sealer in the dentinal tubules: an in vivo study. J Endod 20:373–376CrossRefPubMed Vassiliadis LP, Sklavounos SA, Stavrianos CK (1994) Depth of penetration and appearance of Grossman sealer in the dentinal tubules: an in vivo study. J Endod 20:373–376CrossRefPubMed
46.
go back to reference Mjör IA, Nordhal I (1996) The density and branching of dentinal tubules in human teeth. Arch Oral Biol 41:401–412CrossRefPubMed Mjör IA, Nordhal I (1996) The density and branching of dentinal tubules in human teeth. Arch Oral Biol 41:401–412CrossRefPubMed
47.
go back to reference Beust TB (1931) Reactions of the dentinal fibril to external irritation. J Am Dent Assoc 18:1060–1073 Beust TB (1931) Reactions of the dentinal fibril to external irritation. J Am Dent Assoc 18:1060–1073
48.
go back to reference Vasiliadis I, Darling AI, Levers BGH (1983) The amount and distribution of sclerotic human root dentine. Arch Oral Biol 28:645–649CrossRefPubMed Vasiliadis I, Darling AI, Levers BGH (1983) The amount and distribution of sclerotic human root dentine. Arch Oral Biol 28:645–649CrossRefPubMed
49.
go back to reference Russel AA, Chandler NP, Haumann C, Siddiqui AY, Tompkins GR (2013) The butterfly effect: an investigation of sectioned roots. J Endod 39:208–210CrossRef Russel AA, Chandler NP, Haumann C, Siddiqui AY, Tompkins GR (2013) The butterfly effect: an investigation of sectioned roots. J Endod 39:208–210CrossRef
50.
go back to reference Van Huysen G (1960) The microstructure of normal and sclerosed dentin. J Prosthet Dent 10:976–982CrossRef Van Huysen G (1960) The microstructure of normal and sclerosed dentin. J Prosthet Dent 10:976–982CrossRef
51.
Metadata
Title
Double dye technique and fluid filtration test to evaluate early sealing ability of an endodontic sealer
Authors
Luigi Generali
C. Prati
C. Pirani
F. Cavani
M. R. Gatto
M. G. Gandolfi
Publication date
01-05-2017
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 4/2017
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-016-1878-0

Other articles of this Issue 4/2017

Clinical Oral Investigations 4/2017 Go to the issue