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01-03-2021 | S.I : Translational aspects of Lipid Biology in the Brain

Docosahexaenoic Acid (DHA) Supplementation Alters Phospholipid Species and Lipid Peroxidation Products in Adult Mouse Brain, Heart, and Plasma

Authors: Grace Y. Sun, Michael K. Appenteng, Runting Li, Taeseon Woo, Bo Yang, Chao Qin, Meixia Pan, Magdalena Cieślik, Jiankun Cui, Kevin L. Fritsche, Zezong Gu, Matthew Will, David Beversdorf, Agata Adamczyk, Xianlin Han, C. Michael Greenlief

Published in: NeuroMolecular Medicine | Issue 1/2021

Abstract

The abundance of docosahexaenoic acid (DHA) in phospholipids in the brain and retina has generated interest to search for its role in mediating neurological functions. Besides the source of many oxylipins with pro-resolving properties, DHA also undergoes peroxidation, producing 4-hydroxyhexenal (4-HHE), although its function remains elusive. Despite wide dietary consumption, whether supplementation of DHA may alter the peroxidation products and their relationship to phospholipid species in brain and other body organs have not been explored sufficiently. In this study, adult mice were administered a control or DHA-enriched diet for 3 weeks, and phospholipid species and peroxidation products were examined in brain, heart, and plasma. Results demonstrated that this dietary regimen increased (n-3) and decreased (n-6) species to different extent in all major phospholipid classes (PC, dPE, PE-pl, PI and PS) examined. Besides changes in phospholipid species, DHA-enriched diet also showed substantial increases in 4-HHE in brain, heart, and plasma. Among different brain regions, the hippocampus responded to the DHA-enriched diet showing significant increase in 4-HHE. Considering the pro- and anti-inflammatory pathways mediated by the (n-6) and (n-3) polyunsaturated fatty acids, unveiling the ability for DHA-enriched diet to alter phospholipid species and lipid peroxidation products in the brain and in different body organs may be an important step forward towards understanding the mechanism(s) for this (n-3) fatty acid on health and diseases.

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Title: Docosahexaenoic Acid (DHA) Supplementation Alters Phospholipid Species and Lipid Peroxidation Products in Adult Mouse Brain, Heart, and Plasma
Authors: Grace Y. Sun
Michael K. Appenteng
Runting Li
Taeseon Woo
Bo Yang
Chao Qin
Meixia Pan
Magdalena Cieślik
Jiankun Cui
Kevin L. Fritsche
Zezong Gu
Matthew Will
David Beversdorf
Agata Adamczyk
Xianlin Han
C. Michael Greenlief
Publication date: 01-03-2021
Publisher: Springer US
Published in: NeuroMolecular Medicine / Issue 1/2021
Print ISSN: 1535-1084
Electronic ISSN: 1559-1174
DOI: https://doi.org/10.1007/s12017-020-08616-0

At a glance: The STEP trials

Springer Medicine

Docosahexaenoic Acid (DHA) Supplementation Alters Phospholipid Species and Lipid Peroxidation Products in Adult Mouse Brain, Heart, and Plasma

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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