Top

Published in:

01-04-2010 | Original Article

DNMT3B Promoter Polymorphism and Risk of Gastric Cancer

Authors: Jiabo Hu, Hong Fan, Dongsheng Liu, Shuhong Zhang, Feng Zhang, Huaxi Xu

Published in: Digestive Diseases and Sciences | Issue 4/2010

Abstract

To investigate the association of single-nucleotide polymorphism (SNP) in DNA methyltransferase 3B (DNMT3B) gene and the risk of gastric cancer (GC), we detected -149C>T and -579G>T in the promoter region of the DNMT3B gene by polymerase chain reaction restriction fragment length polymorphism (PCR–RFLP) and DNA sequencing analysis. The DNMT3B genotype was determined in 259 gastric cancer patients and 262 healthy controls that were frequency matched for age and gender. Results showed that individuals with at least one -579G allele were also at significantly decreased risk of gastric cancer [odds ratio (OR), 0.43; 95% confidence interval (CI) 0.26–0.72] compared with those having a -579TT genotype. The -149C>T genotype distribution was irrelevant to the risk of gastric cancer (OR, 1.49; 95% CI, 0.17–17.94) in the studied Chinese population. In addition, data suggested that DNMT3B genetic polymorphism varied among different races, ethnic groups, and geographic areas.

Yang L. Incidence and mortality of gastric cancer in China. World J Gastroenterol. 2006;12:17–20.PubMed

Leung WK, Wu MS, Kakugawa Y, et al. Asia pacific working group on gastric cancer: screening for gastric cancer in Asia: current evidence and practice. Lancet Oncol. 2008;9:279–287. doi:10.1016/S1470-2045(08)70072-X.CrossRefPubMed

Bheemanaik S, Reddy YV, Rao DN. Structure, function and mechanism of exocyclic DNA methyltransferases. Biochem J. 2006;399:177–190. doi:10.1042/BJ20060854.CrossRefPubMed

Suzuki M, Shigematsu H, Shames DS, et al. Methylation and gene silencing of the Ras-related GTPase gene in lung and breast cancers. Ann Surg Oncol. 2007;14:1397–1404. doi:10.1245/s10434-006-9089-6.CrossRefPubMed

Fang JY, Lu R, Mikovits JA, Cheng ZH, Zhu HY, Chen YX. Regulation of hMSH2 and hMLH1 expression in the human colon cancer cell line SW1116 by DNA methyltransferase. Cancer Lett. 2006;233:124–130. doi:10.1016/j.canlet.2005.03.005.CrossRefPubMed

Fang JY, Xiao SD. Alteration of DNA methylation in gastrointestinal carcinogenesis. J Gastroenterol Hepatol. 2001;16:960–968. doi:10.1046/j.1440-1746.2001.02554.x.CrossRefPubMed

Jeltsch A. Molecular enzymology of mammalian DNA methyltransferases. Curr Top Microbiol Immunol. 2006;301:203–225. doi:10.1007/3-540-31390-7_7.CrossRefPubMed

Bachman KE, Rountree MR, Baylin SB. Dnmt3a and Dnmt3b are transcriptional repressors that exhibit unique localization properties to heterochromatin. J Biol Chem. 2001;276:32282–32287. doi:10.1074/jbc.M104661200.CrossRefPubMed

Okano M, Bell DW, Haber DA, Li E. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell. 1999;99:247–257. doi:10.1016/S0092-8674(00)81656-6.CrossRefPubMed

10.

Robertson KD. DNA methylation, methyltransferases, and cancer. Oncogene. 2001;20:3139–3155. doi:10.1038/sj.onc.1204341.CrossRefPubMed

11.

Jones JS, Amos CI, Pande M, et al. DNMT3B polymorphism and hereditary nonpolyposis colorectal cancer age of onset. Cancer Epidemiol Biomarkers Prev. 2006;15:886–891. doi:10.1158/1055-9965.EPI-05-0644.CrossRefPubMed

12.

Montgomery KG, Liu MC, Eccles DM, Campbell IG. The DNMT3B C > T promoter polymorphism and risk of breast cancer in a British population: a case–control study. Breast Cancer Res. 2004;6:R390–R394. doi:10.1186/bcr807.CrossRefPubMed

13.

Shen H, Wang L, Spitz MR, Hong WK, Mao L, Wei Q. A novel polymorphism in human cytosine DNA-methyltransferase-3B promoter is associated with an increased risk of lung cancer. Cancer Res. 2002;62:4992–4995.PubMed

14.

Singal R, Das PM, Manoharan M, Reis IM, Schlesselman JJ. Schlesselman, polymorphisms in the DNA methyltransferase 3B gene and prostate cancer risk. Oncol Rep. 2005;14:569–573.PubMed

15.

Liu Z, Wang L, Wang LE, Sturgis EM, Wei Q. Polymorphisms of the DNMT3B gene and risk of squamous cell carcinoma of the head and neck: a case-control study. Cancer Lett. 2008;268:158–165. doi:10.1016/j.canlet.2008.03.034.CrossRefPubMed

16.

17.

Lee SJ, Jeon HS, Jang JS, et al. DNMT3B polymorphisms and risk of primary lung cancer. Carcinogenesis. 2005;26:403–409. doi:10.1093/carcin/bgh307.CrossRefPubMed

18.

Fan H, Liu DS, Zhang SH, Hu JB, Zhang F, Zhao ZJ. DNMT3B 579 G > T promoter polymorphism and risk of esophagus carcinoma in Chinese. World J Gastroenterol. 2008;14:2230–2234. doi:10.3748/wjg.14.2230.CrossRefPubMed

19.

Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988;16:1215. doi:10.1093/nar/16.3.1215.CrossRefPubMed

20.

Chang KP, Hao SP, Liu CT, et al. Promoter polymorphisms of DNMT3B and the risk of head and neck squamous cell carcinoma in Taiwan: a case–control study. Oral Oncol. 2007;43:345–351. doi:10.1016/j.oraloncology.2006.04.006.CrossRefPubMed

21.

Wang DG, Fan JB, Siao CJ, et al. Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome. Science. 1988;280:1077–1082. doi:10.1126/science.280.5366.1077.CrossRef

22.

Skoog T, van’t Hooft FM, Kallin B, et al. A common functional polymorphism (C → A substitution at position -863) in the promoter region of the tumour necrosis factor alpha (TNF-alpha) gene associated with reduced circulating levels of TNF-alpha. Hum Mol Genet. 1999;8:1443–1449. doi:10.1093/hmg/8.8.1443.CrossRefPubMed

23.

Momparler RL, Bovenzi V. DNA methylation and cancer. J Cell Physiol. 2000;183:145–154. doi:10.1002/(SICI)1097-4652(200005)183:2<145::AID-JCP1>3.0.CO;2-V.CrossRefPubMed

24.

Aung PP, Matsumura S, Kuraoka K, et al. No evidence of correlation between the single nucleotide polymorphism of DNMT3B promoter and gastric cancer risk in a Japanese population. Oncol Rep. 2005;14:1151–1154.PubMed

25.

Wang YM, Wang R, Wen DG, et al. Single nucleotide polymorphism in DNA methyltransferase 3B promoter and its association with gastric cardiac adenocarcinoma in North China. World J Gastroenterol. 2005;11:3623–3627.PubMed

26.

Chen Z, Zhou Z, Chen X, et al. Single nucleotide polymorphism in DNMT3B promoter and the risk for idiopathic thrombocytopenic purpura in Chinese population. J Clin Immunol. 2008;28:399–404. doi:10.1007/s10875-008-9198-z.CrossRefPubMed

27.

Wu Y, Lin JS. DNA methyltransferase 3B promoter polymorphism and its susceptibility to primary hepatocellular carcinoma in the Chinese Han nationality population: a case-control study. World J Gastroenterol. 2007;13:6082–6086. doi:10.3748/wjg.13.6082.CrossRefPubMed

28.

Hong YS, Lee HJ, You CH, et al. DNMT3B 39179GT polymorphism and the risk of adenocarcinoma of the colon in Koreans. Biochem Genet. 2007;45:155–163. doi:10.1007/s10528-006-9047-9.CrossRefPubMed

29.

Wang J, Walsh G, Liu DD, Lee JJ, Mao L. Expression of Delta DNMT3B variants and its association with promoter methylation of p16 and RASSF1A in primary non-small cell lung cancer. Cancer Res. 2006;66:8361–8366. doi:10.1158/0008-5472.CAN-06-2031.CrossRefPubMed

30.

Saito Y, Kanai Y, Sakamoto M, Saito H, Ishii H, Hirohashi S. Overexpression of a splice variant of DNA methyltransferase 3b, DNMT3B4, associated with DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesis. Proc Natl Acad Sci USA. 2002;99:10060–10065. doi:10.1073/pnas.152121799.CrossRefPubMed

31.

Chen T, Ueda Y, Xie S, Li E. A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J Biol Chem. 2002;277:38746–38754. doi:10.1074/jbc.M205312200.CrossRefPubMed

32.

Soejima K, Fang W, Rollins BJ. DNA methyltransferase 3b contributes to oncogenic transformation induced by SV40 T antigen and activated Ras. Oncogene. 2003;22:4723–4733. doi:10.1038/sj.onc.1206510.CrossRefPubMed

33.

Weisenberger DJ, Velicescu M, Cheng JC, Gonzales FA, Liang G, Jones PA. Role of the DNA methyltransferase variant DNMT3B3 in DNA methylation. Mol Cancer Res. 2004;2:62–72.PubMed

34.

Yu Z, Kone BC. Hypermethylation of the inducible nitricoxide synthase gene promoter inhibits its transcription. J Biol Chem. 2004;279:46954–46961. doi:10.1074/jbc.M407192200.CrossRefPubMed

35.

Kim SH, Park J, Choi MC, et al. Zinc-fingers and homeoboxes 1 (ZHX1) binds DNA methyltransferase (DNMT)3B to enhance DNMT3B-mediated transcriptional repression. Biochem Biophys Res Commun. 2007;355:318–323. doi:10.1016/j.bbrc.2007.01.187.CrossRefPubMed

Title: DNMT3B Promoter Polymorphism and Risk of Gastric Cancer
Authors: Jiabo Hu
Hong Fan
Dongsheng Liu
Shuhong Zhang
Feng Zhang
Huaxi Xu
Publication date: 01-04-2010
Publisher: Springer US
Published in: Digestive Diseases and Sciences / Issue 4/2010
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-009-0831-3

Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin

Prof. Florian Limbourg

Prof. Anoop Chauhan

Developed by: Springer Medicine

Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 4/2010

Involvement of Ras and AP-1 in Helicobacter pylori-Induced Expression of COX-2 and iNOS in Gastric Epithelial AGS Cells

A Comparison of Antibody Testing, Permeability Testing, and Zonulin Levels with Small-Bowel Biopsy in Celiac Disease Patients on a Gluten-Free Diet

Frequency and Factors Associated with Small Intestinal Bacterial Overgrowth in Patients with Cirrhosis of the Liver and Extra Hepatic Portal Venous Obstruction

Increased Oxidative Stress, Decreased Total Antioxidant Capacity, and Iron Overload in Untreated Patients with Chronic Hepatitis C

CMV in Moderately Active Colitis: Much Ado About Nothing?

Efficacy of Gastric Electrical Stimulation in Improving Functional Vomiting in Patients with Normal Gastric Emptying

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

At a glance: The STEP trials