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World Journal of Surgical Oncology

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Open Access 01-12-2013 | Research

Disruption of mediator complex subunit 19 (Med19) inhibits cell growth and migration in tongue cancer

Authors: Li-Jun Zhu, Wang-Xiang Yan, Zhong-Wei Chen, Yu Chen, Dan Chen, Tong-Han Zhang, Gui-Qing Liao

Published in: World Journal of Surgical Oncology | Issue 1/2013

Abstract

Background

Mediator complex subunit 19 (Med19) is a critical subunit of the mediator complex that forms a bridge between the transcription factors and RNA polymerase II. Although it has been reported that Med19 plays an important role in stabilizing the whole mediator complex, its biological importance in tongue cancer cell proliferation and migration has not been addressed.

Methods

By using MTT, BrdU incorporation, colony formation, flow cytometric, tumorigenesis and transwell assays, We tested the Med19 role on tongue cancer cell growth and migration.

Results

We demonstrated that lentivirus-mediated Med19 knockdown could arrest tongue cancer cells at G1 phase, inhibit tongue cancer cell proliferation and migration in vitro. The tumorigenicity of Med19 short hairpin RNA (shRNA)-expressing lentivirus infected tongue cancer cells were decreased after inoculating into nude mice.

Conclusions

These results indicate that Med19 plays an important role in tongue cancer proliferation and migration, and suggest possible applications for tongue cancer therapy.

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Title: Disruption of mediator complex subunit 19 (Med19) inhibits cell growth and migration in tongue cancer
Authors: Li-Jun Zhu
Wang-Xiang Yan
Zhong-Wei Chen
Yu Chen
Dan Chen
Tong-Han Zhang
Gui-Qing Liao
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: World Journal of Surgical Oncology / Issue 1/2013
Electronic ISSN: 1477-7819
DOI: https://doi.org/10.1186/1477-7819-11-116

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Disruption of mediator complex subunit 19 (Med19) inhibits cell growth and migration in tongue cancer

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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