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Odontology
Published in: Odontology 2/2011

01-07-2011 | Original Article

Direct pulp capping effect with experimentally developed adhesive resin systems containing reparative dentin-promoting agents on rat pulp: mixed amounts of additives and their effect on wound healing

Authors: Yoshihisa Taira, Koichi Shinkai, Masaya Suzuki, Chikage Kato, Yoshiroh Katoh

Published in: Odontology | Issue 2/2011

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Abstract

This study examined the wound-healing process of exposed rat pulp when treated with experimental adhesive resin systems. The experimental direct pulp capping adhesive resin systems were composed of primer-I, primer-II and an experimental bonding agent. Primer-I was Clearfil SE Bond (CSE) primer containing 1.0 or 5.0 wt% CaCl2, and primer-II was CSE primer containing 0.1, 1.0 or 5.0 wt% compound of equal mole of pA and pB with synthetic peptides derived from dentin matrix protein 1 (DMP1). Primer-I containing 1.0 and 5.0 wt% CaCl2 was assigned to the experimental groups 1–3 and 4–6, respectively. Primer-II containing 0.1, 1.0 or 5.0 wt% compound of pA and pB was assigned to the experimental groups 1 and 4, 2 and 5, and 3 and 6, respectively. In all experimental groups, CSE bond containing 10 wt% hydroxyapatite powder was used as the experimental bonding agent. The positive control teeth were capped with calcium hydroxide preparation (Dycal), and the negative control teeth were capped with CSE. The specimens were alternately stained with Mayer’s H&E and the enhanced polymer one-step staining methods. Experimental groups 1, 4, 5 and 6 showed a higher level of reparative dentin formation compared to the negative control 14 days postoperatively. At 28 days postoperatively, all experimental groups showed the formation of extensive reparative dentin, and experimental groups 4, 5 and 6 demonstrated similar dentin bridge formation as that of the positive control. How quickly reparative dentin formation occurs may depend on the concentration of CaCl2 and the compound of pA and pB in the experimental primer.
Literature
1.
Brännström M, Nyborg H, Strömberg T. Experiments with pulp capping. Oral Surg Oral Med Oral Pathol. 1979;48:347–52.PubMedCrossRef
2.
Stanley HR. Criteria for standardizing and increasing credibility of direct pulp capping studies. Am J Dent. 1998;11:17–34.
3.
Matsuo T, Nakanishi T, Shimizu H, Ebisu S. A clinical study of direct pulp capping applied to carious-exposed pulps. J Endod. 1996;22:551–6.PubMedCrossRef
4.
Schroder U. Effects of calcium hydroxide-containing pulp-capping agents on pulp cell migration, proliferation, and differentiation. J Dent Res. 1985;64:541–8.PubMed
5.
Cox CF, Suzuki S. Re-evaluating pulp protection: calcium hydroxide liners vs. cohesive hybridization. J Am Dent Assoc. 1994;125:823–31.PubMed
6.
Medina VO III, Shinkai K, Shirono M, Tanaka N, Katoh Y. Histopathologic study on pulp response to single-bottle and self-etching adhesive systems. Oper Dent. 2002;27:330–42.PubMed
7.
Cui C, Zhou X, Chen X, Fan M, Bian Z, Chen Z. The adverse effect of self-etching adhesive systems on dental pulp after direct pulp capping. Quintessence Int. 2009;40:26–34.
8.
Akimoto N, Momoi Y, Kohno A, Suzuki S, Otsuki M, Suzuki S, Cox CF. Biocompatibility of Clearfil Liner Bond 2 and Clearfil AP-X system on nonexposed and exposed primate teeth. Quintessence Int. 1998;29:177–88.PubMed
9.
Lu Y, Liu T, Li X, Li H, Pi G. Histologic evaluation of direct pulp capping with a self-etching adhesive and calcium hydroxide in beagles. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102:78–84.CrossRef
10.
Kitasako Y, Ikeda M, Tagami J. Pulpal responses to bacterial contamination following dentin bridging beneath hard-setting calcium hydroxide and self-etching adhesive resin system. Dent Traumatol. 2008;24:201–6.PubMedCrossRef
11.
Costa CAS, Nascimento ABL, Teixeira HM, Fontana U. Response of human dental pulps capped with a self-etching adhesive system. Dent Mater. 2001;17:230–40.CrossRef
12.
Koliniotou-Koumpia E, Tziafas D. Pulpal responses following direct pulp capping of healthy dog teeth with dentine adhesive systems. J Dent. 2005;33:639–47.PubMedCrossRef
13.
Pereira JC, Segala AD, Costa CAS. Human pulpal response to direct pulp capping with an adhesive system. Am J Dent. 2000;13:139–47.PubMed
14.
Silva GAB, Lanza LD, Lopes-Júnior N, Moreira A, Alves JB. Direct pulp capping with a dentin bonding system in human teeth: a clinical and histological evaluation. Oper Dent. 2006;31:297–307.PubMedCrossRef
15.
Katoh Y. Clinico-pathological study on pulp-irritation of adhesive resinous material, report 1—histopathological change of the pulp tissue in direct capping. Adhes Dent. 1993;11:119–211.
16.
Katoh Y. Microscopic observation of the wound healing process of pulp directly capped with adhesive resins. Adhes Dent. 1997;15:229–39.
17.
Katoh Y, Kimura T, Inaba T. Clinicopathological study on pulp-irritation of adhesive resinous materials, report 2—clinical prognosis of the pulp tissue directly capped with resinous materials. Jpn J Conserv Dent. 1997;40:153–62.
18.
Katoh Y, Yamaguchi R, Shinkai K, Hasegawa K, Kimura T, Ebihara T, Suzaki T, Ohara A, Kitamura Y, Tanaka N, Jin C. Clinicopathological study on pulp-irritation of adhesive resinous materials, report 3—direct capping effects on exposed pulp of Macaca fascicularis. Jpn J Conserv Dent. 1997;40:163–76.
19.
Katoh Y, Kimura T, Inaba T. Clinicopathological study on pulp-irritation of adhesive resinous materials, report 4—long term clinical prognosis of pulp tissue directly capped with resinous materials. Jpn J Conserv Dent. 1999;42:989–95.
20.
Ebihara T, Katoh Y. Histopathological study on development of adhesive resinous material containing calcium hydroxide as direct pulp capping agent. Jpn J Conserv Dent. 1996;39:1288–315.
21.
Suzaki T, Katoh Y. Histopathological study on 4-META/MMA-TBB resin containing calcium hydroxide as a direct pulp capping agent. Jpn J Conserv Dent. 1997;40:49–77.
22.
Kitamura Y, Katoh Y. Histopathological study on healing properties of exposed pulp irradiated by laser and capped directly with adhesive resin. Jpn J Conserv Dent. 1999;42:461–77.
23.
Shirono M, Ebihara T, Katoh Y. Effect of CO2 laser irradiation and direct capping with two-step bonding system on exposed pulp of Macaca fascicularis. Jpn J Conserv Dent. 2003;46:761–81.
24.
Kitasako Y, Shibata S, Pereira PN, Tagami J. Short-term dentin bridging of mechanically exposed pulps capped with adhesive resin systems. Oper Dent. 2000;25:155–62.PubMed
25.
Pitt Ford TR, Roberts GJ. Immediate and delayed direct pulp capping with the use of a new visible light-cured calcium hydroxide preparation. Oral Surg Oral Med Oral Pathol. 1991;71:338–42.PubMedCrossRef
26.
Katoh Y, Suzuki M, Kato C, Shinkai K, Ogawa M, Yamauchi J. Observation of calcium phosphate powder mixed with an adhesive monomer experimentally developed for direct pulp capping and as a bonding agent. Dent Mater J. 2010;29:15–24.PubMedCrossRef
27.
Suzuki M, Katsumi A, Watanabe R, Shirono M, Katoh Y. Effects of an experimentally developed adhesive resin system and CO2 laser irradiation on direct pulp capping. Oper Dent. 2005;30:702–18.PubMed
28.
Kato C, Suzuki M, Shinkai K, Katoh Y. Direct pulp-capping effect of experimentally developed adhesive-resin-systems containing reparative dentin-promoting-agents. J Dent Res (CD of abstracts, special issue B). 2008;87:1013.
29.
He G, Dahl T, Veis A, George A. Nucleation of apatite crystals in vitro by self-assembled dentin matrix protein 1. Nat Mater. 2003;2:552–8.PubMedCrossRef
30.
Matsuzaki M, Oida S, Yamauchi J, Asakura T. Synthesis of the peptides from dentin matrix protein 1 and the structural analysis using 13C solid-state NMR. In: Proceedings of 2004 symposium of the Japanese society for biomaterials; 2004. Abstr. No P-120.
31.
Katoh Y, Suzuki M, Ogisu T, Kato C, Shinkai K, Yamauchi J, Asakura T. Direct pulp capping effect with synthetic peptide derivatives of dentin matrix protein 1. J Dent Res (CD of abstracts, special issue B). 2008;87:1014.
32.
Ishimatsu H, Kitamura C, Morotomi T, Tabata Y, Nishihara T, Chen KK, Terashita M. Formation of dentinal bridge on surface of regenerated dental pulp in dentin defects by controlled release of fibroblast growth factor-2 from gelatin hydrogels. J Endod. 2009;35:858–65.PubMedCrossRef
33.
Prescott RS, Alsanea R, Fayad MI, Johnson BR, Wenckus CS, Hao J, John AS, George A. In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice. J Endod. 2008;34:421–6.PubMedCrossRef
34.
Tanaka H. Osteocyte differentiation and DMP1. Bone. 2007;21:761–4.
35.
Narayanan K, Srinivas R, Ramachandran A, Hao J, Quinn B, George A. Differentiation of embryonic mesenchymal cells to odontoblast-like cells by overexpression of dentin matrix protein l. Proc Natl Acad Sci USA. 2001;98:4516–21.PubMedCrossRef
36.
Almushayt A, Narayanan K, Zaki AE, George A. Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts. Gene Ther. 2006;13:611–20.PubMedCrossRef
37.
Ishikawa K, Eanes ED, Asaoka K. Effect of calcium ions on hydroxyapatite formation from the hydrolysis of anhydrous dicalcium phosphate. Dent Mater J. 1994;13:182–9.PubMed
38.
Cox CF, Subay RK, Ostro E, Suzuki S, Suzuki SH. Tunnel defects in dentin bridges: their formation following direct pulp capping. Oper Dent. 1996;21:4–11.PubMed
39.
Hayashi Y, Imai M, Yanagiguchi K, Viloria IL, Ikeda T. Hydroxyapatite applied as direct pulp capping medicine substitutes for osteodentin. J Endod. 1999;25:225–9.PubMedCrossRef
40.
Nakashima M. Induction of dentine in amputated pulp of dogs by recombinant human bone morphogenetic proteins-2 and -4 with collagen matrix. Arch Oral Biol. 1994;39:1085–9.PubMedCrossRef
41.
Hu CC, Zhang C, Qian Q, Tatum NB. Reparative dentin formation in rat molars after direct pulp capping with growth factors. J Endod. 1998;24:744–51.PubMedCrossRef
42.
Datwyler G. Klinische und histologische Untersuchungen über einige Pulpaüberkappungsmethoden. Schweiz Mschr Zahnheilk. 1921;3:291–8.
43.
Kronfeld R. Über den Ausgang traumatischer Pulpenschädigung. Z Stomat. 1929;27:846–66.
44.
Holland R, de Souza V, de Mello W, Nery MJ, Bernabe PFE, Filho JAO. Permeability of the hard tissue bridge formed after pulpotomy with calcium hydroxide, a histological study. J Am Dent Assoc. 1979;99:472–5.PubMed
45.
Kalnins V, Frisbie HE. The effect of dentine fragments on the healing of the exposed pulp. Arch Oral Biol. 1960;2:96–103.PubMedCrossRef
46.
Katoh Y. Hybridized capping effect of two step bonding system and Ca(OH)2 to exposed pulp. In: Ishikawa T, Takahashi K, Maeda T, Suda H, Shimono M, Inoue T, editors. Proceedings of the international conference on dentin/pulp complex 2001. Tokyo: Quintessence Publishing; 2002. p. 95–101.
47.
Senia ES, Marshall FJ, Rosen S. The solvent action of sodium hypochlorite on pulp tissue of extracted teeth. Oral Surg Oral Med Oral Pathol. 1971;31:96–103.PubMedCrossRef
48.
Rosenfeld EF, James GA, Burch BS. Vital pulp tissue response to sodium hypochlorite. J Endod. 1978;4:140–6.PubMedCrossRef
49.
Kitasako Y, Inokoshi S, Tagami J. Effects of direct resin pulp capping techniques on short-term response of mechanically exposed pulps. J Dent. 1999;27:257–63.PubMedCrossRef
50.
Hafez AA, Cox CF, Tarim B, Otsuki M, Akimoto N. An in vivo evaluation of hemorrhage control using sodium hypochlorite and direct capping with a one- or two-component adhesive system in exposed nonhuman primate pulps. Quintessence Int. 2002;33:261–72.PubMed
51.
Costa CAS, Vaertenb MA, Edwardsc CA, Hanks CT. Cytotoxic effects of current dental adhesive systems on immortalized odontoblast cell line MDPC-23. Dent Mater. 1999;15:434–41.PubMedCrossRef
52.
White KC, Cox CF, Kanka J, Dixon DL, Farmer JB, Snuggs HM. Pulpal response to adhesive resin systems applied to acid-etched vital dentin: damp versus dry primer application. Quintessence Int. 1994;25:259–68.PubMed
53.
Huang FM, Chang YC. Cytotoxicity of dentine-bonding agents on human pulp cells in vitro. Int Endod J. 2002;35:905–9.PubMedCrossRef
54.
Shinkai K, Taira Y, Suzuki M, Kato C, Yamauchi J, Suzuki S, Katoh Y. Effect of the concentration of calcium chloride and synthetic peptides in primers on dentin bond strength of an experimental adhesive system. Dent Mater J. 2010;29:738–46.
55.
Sano H, Yoshikawa T, Pereira PN, Kanemura N, Morigami M, Tagami J, Pashley DH. Long-term durability of dentin bonds made with a self-etching primer, in vivo. J Dent Res. 1999;78:906–11.PubMedCrossRef
Metadata
Title
Direct pulp capping effect with experimentally developed adhesive resin systems containing reparative dentin-promoting agents on rat pulp: mixed amounts of additives and their effect on wound healing
Authors
Yoshihisa Taira
Koichi Shinkai
Masaya Suzuki
Chikage Kato
Yoshiroh Katoh
Publication date
01-07-2011
Publisher
Springer Japan
Published in
Odontology / Issue 2/2011
Print ISSN: 1618-1247
Electronic ISSN: 1618-1255
DOI
https://doi.org/10.1007/s10266-011-0006-5

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