Top

Published in:

01-04-2018

Diosmin Prevents Isoproterenol-Induced Heart Mitochondrial Oxidative Stress in Rats

Authors: S. Sharmila Queenthy, P. Stanely Mainzen Prince, Babu John

Published in: Cardiovascular Toxicology | Issue 2/2018

Abstract

Cardiac mitochondrial oxidative stress causes mitochondrial damage that plays an important role in the pathology of myocardial infarction. The preventive effects of diosmin on cardiac mitochondrial oxidative stress in isoproterenol-induced myocardial infarcted rats were evaluated. Rats were pretreated with diosmin (10 mg/kg body weight) daily for 10 days. Myocardial infarction was induced in rats by isoproterenol (100 mg/kg body weight) injection twice at an interval of 24 h (on 11th and 12th day). Isoproterenol-induced myocardial infarcted rats showed a significant increase in the levels of cardiac diagnostic markers, heart mitochondrial lipid peroxidation, calcium ion, and a significant decrease in the levels of heart mitochondrial glutathione peroxidase, reduced glutathione, glutathione-S-transferase, isocitrate, malate, α-ketoglutarate, and succinate dehydrogenases. Transmission electron microscopic findings revealed damaged mitochondria with loss of cristae, swelling, and vacuolation in isoproterenol-induced rats’ heart. Diosmin pretreatment showed significant preventive effects on all the biochemical parameters, and the structure of mitochondria was evaluated. Furthermore, the transmission electron microscopic study confirms the biochemical findings. The antioxidant and negative inotropic effects of diosmin inhibited cardiac mitochondrial oxidative stress and prevented mitochondrial damage in myocardial infarcted rats.

Rajadurai, M., & Stanely Mainzen Prince, P. (2007). Preventive effect of naringin on isoproterenol induced cardiotoxicity in Wistar rats: An in vivo and in vitro study. Toxicology, 232, 216–225.CrossRefPubMed

Wang, J., Bo, H., Meng, X., Wu, Y., Bao, Y., & Li, Y. (2006). A simple and fast experimental model of myocardial infarction in the mouse. Texas Heart Institute Journal, 33, 290–293.PubMedPubMedCentral

Rathore, N., John, S., Kale, M., & Bhatnagar, D. (1998). Lipid peroxidation and antioxidant enzymes in isoproterenol induced oxidative stress in rat tissues. Pharmacological Research, 38, 97–303.CrossRef

Srivastava, S., Chandrasekar, B., Gu, Y., Luo, J., Hamid, T., Hill, B. G., et al. (2007). Down regulation of CuZn-superoxide dismutase contributes to β-adrenergic receptor-mediated oxidative stress in the heart. Cardiovascular Research, 74, 445–455.CrossRefPubMed

Rajadurai, M., & Stanely Mainzen Prince, P. (2006). Preventive effect of naringin on lipid peroxides and antioxidants in isoproterenol-induced cardiotoxicity in Wistar rats: Biochemical and histopathological evidences. Toxicology, 228, 259–268.CrossRefPubMed

Senthil Kumaran, K., & Stanely Mainzen Prince, P. (2010). Caffeic acid protects rat heart mitochondria against isoproterenol-induced oxidative damage. Cell Stress and Chaperones, 15, 791–806.CrossRef

Ho, C. T., Osawa, T., Huang, M. T., & Rosen, R. T. (Eds.) (1994). Food phytochemicals and cancer preventions II. Teas, Spices and Herbs, ACS symposium series 547, American Chemical Society, Washington, DC.

Sharmila Queenthy, S., & John, Babu. (2013). Diosmin exhibits anti-hyperlipidemic effects in isoproterenol induced myocardial infarcted rats. European Journal of Pharmacology, 718, 213–218.CrossRefPubMed

Kuntz, S., Wenzel, U., & Daniel, H. (1999). Comparative analysis of the effects of flavonoids on proliferation, cytotoxicity and apoptosis in human colon cancer cell lines. European Journal of Nutrition, 38, 133–142.CrossRefPubMed

10.

Murray, A. J., Edwards, L. M., & Clarke, K. (2007). Mitochondria and heart failure. Current Opinion in Clinical Nutrition and Metabolic Care, 10, 704–711.CrossRefPubMed

11.

Stanely Mainzen Prince, P. (2011). A biochemical, electrocardiographic, electrophoretic, histopathological and in vitro study on the protective effects of (–) epicatechin in isoproterenol induced myocardial infarcted rats. European Journal of Pharmacology, 67, 195–201.

12.

Takasawa, M., Hayakawa, M., Sugiyama, S., Hattori, K., Ito, T., & Ozawa, T. (1993). Age-associated damage in mitochondrial function in rat hearts. Experimental Gerontology, 28, 269–280.CrossRefPubMed

13.

Fraga, C. G., Leibovitz, B. E., & Tappel, A. L. (1988). Lipid peroxidation measured as thiobarbituric acid reactive substances in tissue slices: Characterization and comparison with homogenate and microsomes. Free Radical Biology and Medicine, 4, 155–161.CrossRefPubMed

14.

Kakkar, P., Das, B., & Viswanathan, P. N. (1984). A modified spectrophotometric assay of superoxide dismutase. Indian Journal of Biochemistry and Biophysics, 21, 130–132.PubMed

15.

Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G., & Hoekstra, W. G. (1973). Selenium: Biochemical role as a component of glutathione peroxidase. Science, 179, 588–590.CrossRefPubMed

16.

Ellman, G. L. (1959). Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 82, 70–77.CrossRefPubMed

17.

Habig, W. H., & Jakoby, W. B. (1981). Assays for differentiation of glutathione-S-transferase. Methods in Enzymology, 77, 398–405.CrossRefPubMed

18.

King, J. (1965). Isocitrate dehydrogenase. In J. C. King & D. Van (Eds.), Practical clinical enzymology (p. 363). London: Nostrand Co.

19.

Mehler, A. H., Kornberg, A., Grisolia, S., & Ochoa, S. (1948). The enzymatic mechanims of oxidation-reductions between malate or isocitrate or pyruvate. Journal of Biological Chemistry, 174, 961–977.PubMed

20.

Reed, L. J., & Mukherjee, R. B. (1969). Alpha ketoglutarate dehydrogenase complex from Escherichia coli. In J. M. Lowenstein (Ed.), Methods in enzymology (pp. 53–61). London: Academic.

21.

Slater, E. C., & Jr Borner, W.D. (1952). The effect of fluoride on the succinic oxidase system. Biochemistry Journal, 52, 185–196.CrossRef

22.

Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin’s—Phenol reagent. Journal of Biological Chemistry, 193, 265–275.PubMed

23.

Benzie, F. F., & Strain, J. J. (1999). Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299, 15–23.CrossRefPubMed

24.

Sangeetha, T., & Darlin Quine, S. (2008). Protective effect of S-allyl cysteine sulphoxide (alliin) on glycoproteins and hematology in isoproterenol induced myocardial infarction in male Wistar rats. Journal of Applied Toxicology, 28, 710–716.CrossRefPubMed

25.

Stanely Mainzen Prince, P., Dhanasekar, K., & Rajkumar, S. (2011). Preventive effects of vanillic acid on lipids, Bax, Bcl-2 and myocardial infarct size on isoproterenol-induced myocardial infarcted rats: A biochemical and in vitro study. Cardiovascular Toxicology, 11, 58–66.CrossRefPubMed

26.

Thomes, P., Rajendran, M., Pasanban, B., & Rengasamy, R. (2010). Cardioprotective activity of Cladosiphon okamuranus fucoidan against isoproterenol induced myocardial infarction in rats. International Journal of Phytotherapy and Phytopharmacology, 18, 52–57.CrossRef

27.

Punithavathi, V. R., & Stanely Mainzen Prince, P. (2010). Pretreatment with a combination of quercetin and α-tocopherol ameliorates adenosine triphosphatases and lysosomal enzymes in myocardial infarcted rats. Life Sciences, 86, 178–184.CrossRefPubMed

28.

Saeed, S. A., & Ahmed, S. (2006). Role of cyclooxygenase-2 in myocardial infarction and ischemia. Journal of the College of Physicians and Surgeons Pakistan, 16, 324–328.PubMed

29.

Karthikeyan, K., Sarala Bai, B. R., & Niranjali Devaraj, S. (2007). Grape seed proanthocyanidins ameliorates isoproterenol-induced myocardial injury in rats by stabilizing mitochondrial and lysosomal enzymes: An in vivo study. Life Sciences, 81, 1615–1621.CrossRefPubMed

30.

Zhou, R., Xu, Q., Zheng, P., Yan, L., Zheng, J., & Dai, G. (2008). Cardioprotective effect of fluvastatin on isoproterenol-induced myocardial infarction in rat. European Journal of Pharmacology, 586, 244–250.CrossRefPubMed

31.

Patel, V., Upaganlawar, A., Zalawadia, R., & Balaraman, R. (2010). Cardioprotective effect of melatonin against isoproterenol induced myocardial infarction in rats: A biochemical, electrocardiographic and histoarchitectural evaluation. European Journal of Pharmacology, 644, 160–168.CrossRefPubMed

32.

Stanely Mainzen Prince, P. (2013). (–) Epicatechin prevents alterations in lysosomal glycohydrolases, cathepsins and reduces myocardial infarct size in isoproterenol-induced myocardial infarcted rats. European Journal of Pharmacology, 706, 63–69.CrossRef

33.

Halliwell, B., & Gutteridge, J. M. (1990). Role of free radicals and catalytic metal ions in human disease: An overview. Methods in Enzymology, 18, 61–85.

34.

Devika, P. T., & Stanely Mainzen Prince, P. (2008). (–) Epigallocatechin-gallate (EGCG) prevents mitochondrial damage in isoproterenol-induced cardiac toxicity in albino Wistar rats: A transmission electron microscopic and in vitro study. Pharmacological Research, 57, 351–357.CrossRefPubMed

35.

Hayes, J. D., & Pulford, D. J. (1995). The glutathione-S-transferase supergene family: Regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Critical Reviews in Biochemistry and Molecular Biology, 30, 445–600.CrossRefPubMed

36.

Sangeetha, T., & Darlin Quine, S. (2009). Preventive effect of S-allyl cysteine sulphoxide (Alliin) on mitochondrial dysfunction in normal and isoproterenol induced cardio toxicity in male Wistar rats: A histopathological study. Molecular and Cellular Biochemistry, 328, 1–8.CrossRefPubMed

37.

Stanely Mainzen Prince, P. (2013). (–) Epicatechin attenuates mitochondrial damage by enhancing mitochondrial multi-marker enzymes, adenosine triphosphate and lowering calcium in isoproterenol induced myocardial infarcted rats. Food and Chemical Toxicology, 53, 409–416.CrossRefPubMed

38.

Ma, Y. L., & Ma, C. (2007). Studies on pharmacokinetics of diosmin in rats. Zhongguo Zhongyao Zazhi, 32, 418–420.PubMed

Title: Diosmin Prevents Isoproterenol-Induced Heart Mitochondrial Oxidative Stress in Rats
Authors: S. Sharmila Queenthy
P. Stanely Mainzen Prince
Babu John
Publication date: 01-04-2018
Publisher: Springer US
Published in: Cardiovascular Toxicology / Issue 2/2018
Print ISSN: 1530-7905
Electronic ISSN: 1559-0259
DOI: https://doi.org/10.1007/s12012-017-9422-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Diosmin Prevents Isoproterenol-Induced Heart Mitochondrial Oxidative Stress in Rats

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2018

The Effects of Periostin in a Rat Model of Isoproterenol: Mediated Cardiotoxicity

Unanticipated Cardiotoxicity Associated with Targeted Anticancer Therapy in Patients with Hematologic Malignancies Patients: Natural History and Risk Factors

Asiatic Acid Ameliorates Doxorubicin-Induced Cardiac and Hepato-Renal Toxicities with Nrf2 Transcriptional Factor Activation in Rats

A Case of Severe, Refractory Hypotension After Amlodipine Overdose

Quercetin Reverses Altered Energy Metabolism in the Heart of Rats Receiving Adriamycin Chemotherapy

Predicting Cardiotoxic Effects of Carbon Monoxide Poisoning Using Speckle Tracking Echocardiography