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Digestive Diseases and Sciences
Published in: Digestive Diseases and Sciences 6/2007

01-06-2007 | Original Paper

Dietary Glutamine Supplementation Prevents Mucosal Injury and Modulates Intestinal Epithelial Restitution Following Ischemia-Reperfusion Injury in the Rat

Authors: Igor Sukhotnik, Kamal Khateeb, Jorge G. Mogilner, Habib Helou, Michael Lurie, Arnold G. Coran, Eitan Shiloni

Published in: Digestive Diseases and Sciences | Issue 6/2007

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Abstract

The aim of the present study was to evaluate the preventive effect of a 2-day oral glutamine supplementation against intestinal ischemia-reperfusion (IR) injury in a rat. Male Sprague-Dawley rats were divided into four experimental groups: sham rats underwent laparotomy, sham-GLU rats underwent laparotomy and were treaded with enteral glutamine (GLU) given in drinking water (2%) 48 hr before and following operation, IR rats underwent occlusion of both the superior mesenteric artery and the portal vein for 30 min followed by 24 hr of reperfusion, and IR-GLU rats were treated with enteral glutamine 48 hr before and following IR. Intestinal mucosal damage (Park’s injury score), mucosal structural changes, enterocyte proliferation, and enterocyte apoptosis were determined 24 hr following IR. Sham-GLU rats demonstrated a lower rate of cell apoptosis in jejunum and ileum compared to sham animals. IR-GLU animals demonstrated a greater jejunal and ileal bowel and mucosal weight, mucosal DNA, villous height and crypt depth, and enterocyte proliferation index in ileum and a lower injury score grade in jejunum compared to IR-nontreated rats. In conclusion, pretreatment with oral glutamine prevents mucosal injury and improves intestinal recovery following IR injury in the rat.
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Metadata
Title
Dietary Glutamine Supplementation Prevents Mucosal Injury and Modulates Intestinal Epithelial Restitution Following Ischemia-Reperfusion Injury in the Rat
Authors
Igor Sukhotnik
Kamal Khateeb
Jorge G. Mogilner
Habib Helou
Michael Lurie
Arnold G. Coran
Eitan Shiloni
Publication date
01-06-2007
Published in
Digestive Diseases and Sciences / Issue 6/2007
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI
https://doi.org/10.1007/s10620-006-9629-8

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