Top

BMC Complementary Medicine and Therapies

Published in:

Open Access 01-12-2024 | Diabetic Foot | Research

Exploring the phytoconstituents, antimicrobial potency, and cytotoxic effects of essential oil from Origanum punonense from Palestine

Authors: Kamal Issa, Amjad Bakhatan, Majde Abu Khaled, Nidal Jaradat, Mohammed Hawash, Nawaf Al-Maharik, Mustafa Ghanim, Mohammad Qadi

Published in: BMC Complementary Medicine and Therapies | Issue 1/2024

Abstract

Background

Origanum punonense Danin is one of the old traditional medicinal plants Bedouins utilize in the Dead Sea region to treat a variety of illnesses, those caused by infections. The current study aimed to identify the phytochemical components of O. punonense essential oil (EO) and determine its antiproliferative and antimicrobial effects.

Methods

Gas chromatography and mass spectrometry were employed to detect the phytochemical constituents of O. punonense EO. Broth microdilution assay was utilized to determine the antimicrobial effects against various microbial species, including those causing diabetic foot infections.

Results

This study revealed that O. punonense EO contains 44 phytochemical compounds, of which 41 compounds were detectable and amounted to 99.78% of the total oil. The main chemical components of the oil were carvacrol (57.4%), p-cymene (6.66%), carvone (5.35%), pinene (4.9%), and terpinene (2.96%). The antiproliferative activity of different concentrations of O. punonense EO was noted in all of the investigated cell lines, with the best activity at the concentration of 500 µg/mL. The greatest antibacterial activity was against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Proteus vulgaris, with MIC values of 1.56 µL/mL. In addition, and the O. punonense EO showed strong antifungal activity against Candida albicans with a MIC value of 0.8 µL/mL. In addition, the O. punonense EO showed potent antibacterial activity against all MRSA samples obtained from the diabetic foot with a MIC value of 3.13 µL/mL. The O. punonense EO demonstrated potent activity against Carbapenem-resistant Enterobacterales, Citrobacter freundii, and K. pneumoniae, with MICs value of 6.25 µL/mL.

Conclusion

The potent antiproliferative and broad antimicrobial activity of O. punonense EO makes it an effective strategy for treating infections, especially in immunocompromised patients with chronic comorbidities such as cancer and diabetes mellitus.

Sam S. Importance and effectiveness of herbal medicines. J Pharmacognosy Phytochemistry. 2019;8(2):354–7.

Kumari R, Kotecha M. A review on the standardization of herbal medicines. Int J Pharma Sci Res. 2016;7(2):97–106.

Raut JS, Karuppayil SM. A status review on the medicinal properties of essential oils. Ind Crops Prod. 2014;62:250–64.CrossRef

Irshad M, Subhani MA, Ali S, Hussain A. Biological importance of essential oils. Essent Oils-Oils Nat. 2020;1:37–40.

Jaradat N, Al-Lahham S, Abualhasan MN, Bakri A, Zaide H, Hammad J, Hussein F, Issa L, Mousa A, Speih R. Chemical constituents, antioxidant, cyclooxygenase inhibitor, and cytotoxic activities of Teucrium pruinosum boiss. Essential oil. BioMed Research International 2018, 2018.

Harley RM, Atkins S, Budantsev AL, Cantino PD, Conn BJ, Grayer R, Harley MM. De Kok Rd, Krestovskaja Td, Morales R: Labiatae. Flowering Plants· Dicotyledons: Lamiales (except Acanthaceae including Avicenniaceae). edn.: Springer; 2004. pp. 167–275.

Chakrabartty I, Mohanta YK, Nongbet A, Mohanta TK, Mahanta S, Das N, Saravanan M, Sharma N. Exploration of Lamiaceae in cardio vascular diseases and functional foods: Medicine as food and food as medicine. Front Pharmacol. 2022;13:894814.CrossRefPubMedPubMedCentral

Ietswaart JH. A taxonomic revision of the genus Origanum (Labiatae). Leiden Bot Ser. 1980;4(1):1–153.

Shtayeh MSA, Jamous RM. Traditional arabic Palestinian Herbal Medicine. Palestine: BERC; 2008.

10.

Danin A. Two new species of Origanum (Labiatae) from Jordan. Willdenowia 1990:401–4.

11.

Frazier MS, Fuqua T. Essentials of Human diseases and conditions-E-Book. Elsevier Health Sciences; 2020.

12.

Yusuf K, Sampath V, Umar S. Bacterial infections and Cancer: Exploring this Association and its implications for Cancer patients. Int J Mol Sci. 2023;24(4):3110.CrossRefPubMedPubMedCentral

13.

Parija SC. Microbial infections. Textbook of Microbiology and Immunology. edn.: Springer; 2023. pp. 73–90.

14.

Fagin JA, Krishnamoorthy GP, Landa I. Pathogenesis of cancers derived from thyroid follicular cells. Nat Rev Cancer. 2023;23(9):631–50.CrossRefPubMedPubMedCentral

15.

Armstrong DG, Tan T-W, Boulton AJ, Bus SA. Diabetic foot ulcers: a review. JAMA. 2023;330(1):62–75.CrossRefPubMed

16.

Geng K, Ma X, Jiang Z, Huang W, Gao C, Pu Y, Luo L, Xu Y, Xu Y. Innate immunity in diabetic wound healing: focus on the mastermind hidden in chronic inflammatory. Front Pharmacol. 2021;12:653940.CrossRefPubMedPubMedCentral

17.

Jaradat N. Quantitative estimations for the volatile Oil by using hydrodistillation and microwave accelerated distillation methods from Ruta graveolens L. and Ruta chalepensis L. leaves from Jerusalem area/Palestine. Moroccan Journal of Chemistry 2016, 4(1):4 – 1 (2016) 2011–2016.

18.

Al-Hamwi M, Bakkour Y, Abou-Ela M, El-Lakany A, Tabcheh M, El-Omar F. Chemical composition and seasonal variation of the essential oil of Micromeria fruticosa. J Nat Prod. 2011;4(2011):147–9.

19.

Jaradat NA, Zaid AN, Abuzant A, Shawahna R. Investigation the efficiency of various methods of volatile oil extraction from Trichodesma Africanum and their impact on the antioxidant and antimicrobial activities. J Intercultural Ethnopharmacol. 2016;5(3):250.CrossRef

20.

Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal. 2016;6(2):71–9.CrossRefPubMed

21.

Rahhal B, Qneibi M, Jaradat N, Hawash M, Qadi M, Issa L, Bdir S. Multi-biological activity assessment and phytochemical characterization of an aqueous extract of the Cymbopogon citratus grown in Palestine. BMC Complement Med Ther. 2024;24(1):1–12.CrossRef

22.

Shehadeh M, Jaradat N, Al-Masri M, Zaid AN, Hussein F, Khasati A, Suaifan G, Darwish R. Rapid, cost-effective and organic solvent-free production of biologically active essential oil from Mediterranean wild Origanum syriacum. Saudi Pharm J. 2019;27(5):612–8.CrossRefPubMedPubMedCentral

23.

Qadi M, Jaradat N, Al-Maharik N, Hawash M, Abdalrazeq M, Fuqha A, Jabareen D, Atamni N, Zarour A. Anticandidal effects and chemical compositions of volatile oils extracted from Origanum syriacum, Clinopodium Serpyllifolium subsp. fruticosum and Thymbra capitata from Palestine. Chem Biol Technol Agric. 2023;10(1):87.CrossRef

24.

Aligiannis N, Kalpoutzakis E, Mitaku S, Chinou IB. Composition and antimicrobial activity of the essential oils of two Origanum species. J Agric Food Chem. 2001;49(9):4168–70.CrossRefPubMed

25.

Afifi FU, Kasabri V, Beltran S, Abuhammad A, Abaza IF, Ganado O, Al-Gabbiesh AH. Comparison of different methods in determination of essential oil composition of Origaum Syriacum L. from Jordan and its modulation of pancreatic enzymes. Rev Roum Chim. 2017;62(1):15–21.

26.

Sellami IH, Maamouri E, Chahed T, Wannes WA, Kchouk ME, Marzouk B. Effect of growth stage on the content and composition of the essential oil and phenolic fraction of sweet marjoram (Origanum majorana L). Ind Crops Prod. 2009;30(3):395–402.CrossRef

27.

Mohammed E, Fliou J, Riffi O, Amechrouq A, Ghouati Y. Comparative study of the chemical composition of the essential oil of Origanum compactum from the seven regions of Morocco and their antimicrobial activity. J Microbiol Biotechnol food Sci. 2020;10(1):42–8.CrossRef

28.

Berber D, Inanc L, Turkmenoglu L, Toksoz O, Sesal N. Antibacterial Potency for Mimosa, Quebracho and essential oils of Origanum species against Acinetobacter pittii, Klebsiella pneumoniae and Bacillus cereus from Diabetic Foot patient. J Am Leather Chem Assoc 2022, 117(6).

29.

Helander IM, Alakomi H-L, Latva-Kala K, Mattila-Sandholm T, Pol I, Smid EJ, Gorris LG, von Wright A. Characterization of the action of selected essential oil components on Gram-negative bacteria. J Agric Food Chem. 1998;46(9):3590–5.CrossRef

30.

Vardar-Ünlü G, Candan F, Sökmen A, Daferera D, Polissiou M, Sökmen M, Dönmez E, Tepe B. Antimicrobial and antioxidant activity of the essential oil and methanol extracts of Thymus pectinatus Fisch. Et Mey. Var. pectinatus (Lamiaceae). J Agric Food Chem. 2003;51(1):63–7.CrossRefPubMed

31.

Pérez-Recalde M, Arias IER, Hermida ÉB. Could essential oils enhance biopolymers performance for wound healing? A systematic review. Phytomedicine: Int J Phytotherapy Phytopharmacology. 2018;38:57–65.CrossRef

32.

Sharifi-Rad M, Berkay Yılmaz Y, Antika G, Salehi B, Tumer TB, Kulandaisamy Venil C, Das G, Patra JK, Karazhan N, Akram M. Phytochemical constituents, biological activities, and health‐promoting effects of the genus Origanum. Phytother Res. 2021;35(1):95–121.CrossRefPubMed

33.

Sampaio LA, Pina LTS, Serafini MR, Tavares DS, Guimaraes AG. Antitumor effects of carvacrol and thymol: a systematic review. Front Pharmacol. 2021;12:702487.CrossRefPubMedPubMedCentral

34.

Arunasree K. Anti-proliferative effects of carvacrol on a human metastatic breast cancer cell line, MDA-MB 231. Phytomedicine: Int J Phytotherapy Phytopharmacology. 2010;17(8–9):581–8.CrossRef

Title: Exploring the phytoconstituents, antimicrobial potency, and cytotoxic effects of essential oil from Origanum punonense from Palestine
Authors: Kamal Issa
Amjad Bakhatan
Majde Abu Khaled
Nidal Jaradat
Mohammed Hawash
Nawaf Al-Maharik
Mustafa Ghanim
Mohammad Qadi
Publication date: 01-12-2024
Publisher: BioMed Central
Keyword: Diabetic Foot
Published in: BMC Complementary Medicine and Therapies / Issue 1/2024
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-024-04395-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Exploring the phytoconstituents, antimicrobial potency, and cytotoxic effects of essential oil from Origanum punonense from Palestine

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 1/2024

Two rare flavonoid glycosides from Litsea glutinosa (Lour.) C. B. Rob.: experimental and computational approaches endorse antidiabetic potentiality

Correction: Exploring the antibacterial, antidiabetic, and anticancer potential of Mentha arvensis extract through in‑silico and in‑vitro analysis

Delphinidins from Maqui Berry (Aristotelia chilensis) ameliorate the subcellular organelle damage induced by blue light exposure in murine photoreceptor-derived cells

A multisite feasibility randomized clinical trial of mindfulness-based resilience training for aggression, stress, and health in law enforcement officers

Anticancer properties and metabolomic profiling of Shorea roxburghii extracts toward gastrointestinal cancer cell lines

Are Tai Chi and Qigong effective in the treatment of traumatic brain injury? A systematic review