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Archives of Virology
Published in: Archives of Virology 7/2020

01-07-2020 | Brief Report

Development and validation of a solid-phase competition ELISA based on virus-like particles of foot-and-mouth disease virus serotype A for antibody detection

Authors: Yunjing Zhang, He Yan, Yali Yao, Suling Zhang, Yan Xiao, Xin Xu, Baicheng Huang, Kegong Tian

Published in: Archives of Virology | Issue 7/2020

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Abstract

Foot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious epidemic disease, which is controlled primarily by prophylactic vaccination and serological monitoring after vaccination. Here, we have developed a solid-phase competition ELISA (SPCE) method based on virus-like particles (VLPs) of FMDV serotype A. The use of VLPs in the SPCE assay as a replacement for inactivated FMDV provides a high level of biosafety. The SPCE showed high concordance rates when compared with the virus neutralization test and liquid-phase blocking ELISA for testing clinical serum samples and successive serological monitoring (kappa = 0.925). Thus, this SPCE is an alternative method for post-immunization detection of antibodies against FMDV serotype A, with high specificity and sensitivity.
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Metadata
Title
Development and validation of a solid-phase competition ELISA based on virus-like particles of foot-and-mouth disease virus serotype A for antibody detection
Authors
Yunjing Zhang
He Yan
Yali Yao
Suling Zhang
Yan Xiao
Xin Xu
Baicheng Huang
Kegong Tian
Publication date
01-07-2020
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 7/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-020-04641-3

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