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01-09-2018 | Brief Report

Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71

Authors: Dan Yin, Yanan Zhu, Kaifeng Wang, Jing Wang, Xiru Zhang, Min Han, Yaqing He, Qing Chen, Guifang Hu

Published in: Archives of Virology | Issue 9/2018

Abstract

Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The 95% detection limit of the RT-RPA was 3.767 log10 genomic copies (LGC)/reaction. The specificity was 100%. In a clinical sample evaluation, this approach demonstrated sufficient clinical performance when compared with a commercial RT-qPCR diagnostic kit. Thus, the RT-RPA assay may be a promising alternative for the detection of EV71-C4.

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Title: Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71
Authors: Dan Yin
Yanan Zhu
Kaifeng Wang
Jing Wang
Xiru Zhang
Min Han
Yaqing He
Qing Chen
Guifang Hu
Publication date: 01-09-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 9/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-3859-x

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