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Clinical Oral Investigations

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Open Access 01-01-2017 | Original Article

Dentifrices for children differentially affect cell viability in vitro

Authors: Barbara Cvikl, Adrian Lussi, Andreas Moritz, Reinhard Gruber

Published in: Clinical Oral Investigations | Issue 1/2017

Abstract

Objectives

Child dentifrices vary in their composition, with possible differential impacts on cells in the oral soft tissue. While cytotoxicity studies have been performed on adult dentifrices, no respective studies have thus far been reported on child dentifrices.

Material and methods

Seventeen commercial dentifrices for children up to 12 years of age were evaluated with respect to their in vitro cytotoxicity on gingival fibroblasts, oral squamous cell carcinoma HSC-2 cells, and L929 mouse fibroblasts. Proliferation was analyzed and live-dead staining was performed.

Results

Ten child dentifrices greatly reduced cell viability with LC50 values below 5 %. Four dentifrices showed a moderate cytotoxicity with LC50 values between 5 and 20 %. Three child dentifrices showed almost no cytotoxicity with LC50 values above 95 %. The results of the assays for proliferation and live-dead staining supported these findings.

Conclusions

The different composition of the child dentifrices translated into a broad spectrum of in vitro cytotoxicity on cells of the oral cavity.

Clinical relevance

The in vitro data provide the scientific foundation for further in vivo research testing the clinical relevance of the present findings.

Li Y, Wang W (2002) Predicting caries in permanent teeth from caries in primary teeth: an eight-year cohort study. J Dent Res 81(8):561–566CrossRefPubMed

Walsh T, Worthington HV, Glenny AM, Appelbe P, Marinho VC, Shi X (2010) Fluoride toothpastes of different concentrations for preventing dental caries in children and adolescents. Cochrane Database Syst Rev 1:CD007868. doi:10.1002/14651858.CD007868.pub2

Creeth J, Bosma ML, Govier K (2013) How much is a ‘pea-sized amount’? A study of dentifrice dosing by parents in three countries. Int Dent J 63(Suppl 2):25–30. doi:10.1111/idj.12074 CrossRefPubMed

Levy SM (1993) A review of fluoride intake from fluoride dentifrice. ASDC J Dent Child 60(2):115–124PubMed

Bennadi D, Kshetrimayum N, Sibyl S, Reddy CV (2014) Toothpaste Utilization Profiles among Preschool children. J Clin Diagn Res 8(3):212–215. doi:10.7860/JCDR/2014/7309.4165 PubMedPubMedCentral

Naccache H, Simard PL, Trahan L, Brodeur JM, Demers M, Lachapelle D, Bernard PM (1992) Factors affecting the ingestion of fluoride dentifrice by children. J Public Health Dent 52(4):222–226CrossRefPubMed

Emling RC, Flickinger KC, Cohen DW, Yankell SL (1981) A comparison of estimated versus actual brushing time. Pharmacol Ther Dent 6(3–4):93–98PubMed

Lynch RJ (2013) The primary and mixed dentition, post-eruptive enamel maturation and dental caries: a review. Int Dent J 63(Suppl 2):3–13. doi:10.1111/idj.12076 CrossRefPubMed

Stovell AG, Newton BM, Lynch RJ (2013) Important considerations in the development of toothpaste formulations for children. Int Dent J 63(Suppl 2):57–63. doi:10.1111/idj.12083 CrossRefPubMed

10.

European Academy of Paediatric D (2009) Guidelines on the use of fluoride in children: an EAPD policy document. Eur Arch Paediatr Dent 10(3):129–135CrossRef

11.

Haftenberger M, Viergutz G, Neumeister V, Hetzer G (2001) Total fluoride intake and urinary excretion in German children aged 3-6 years. Caries Res 35(6):451–457CrossRefPubMed

12.

Maguire A, Zohouri FV, Hindmarch PN, Hatts J, Moynihan PJ (2007) Fluoride intake and urinary excretion in 6- to 7-year-old children living in optimally, sub-optimally and non-fluoridated areas. Community Dent Oral Epidemiol 35(6):479–488. doi:10.1111/j.1600-0528.2006.00366.x CrossRefPubMed

13.

Wong MC, Clarkson J, Glenny AM, Lo EC, Marinho VC, Tsang BW, Walsh T, Worthington HV (2011) Cochrane reviews on the benefits/risks of fluoride toothpastes. J Dent Res 90(5):573–579. doi:10.1177/0022034510393346 CrossRefPubMed

14.

Lawrence LM, Farquharson A, Brown RS, Vatanka HO (2013) Oral tissue irritants in toothpaste: a case report. J Clin Pediatr Dent 38(1):75–78CrossRefPubMed

15.

Herlofson BB, Barkvoll P (1996) Oral mucosal desquamation caused by two toothpaste detergents in an experimental model. Eur J Oral Sci 104(1):21–26CrossRefPubMed

16.

Skaare AB, Rolla G, Barkvoll P (1997) The influence of triclosan, zinc or propylene glycol on oral mucosa exposed to sodium lauryl sulphate. Eur J Oral Sci 105(5 Pt 2):527–533CrossRefPubMed

17.

Cvikl B, Lussi A, Gruber R (2015) The in vitro impact of toothpaste extracts on cell viability. Eur J Oral Sci 123(3):179–185. doi:10.1111/eos.12177 CrossRefPubMed

18.

Creeth JE, Gallagher A, Sowinski J, Bowman J, Barrett K, Lowe S, Patel K, Bosma ML (2009) The effect of brushing time and dentifrice on dental plaque removal in vivo. J Dent Hyg 83(3):111–116PubMed

19.

Winterfeld T, Schlueter N, Harnacke D, Illig J, Margraf-Stiksrud J, Deinzer R, Ganss C (2015) Toothbrushing and flossing behaviour in young adults—a video observation. Clin Oral Investig 19(4):851–858. doi:10.1007/s00784-014-1306-2 CrossRefPubMed

20.

Churchley D, Schemehorn BR (2013) In vitro assessment of a toothpaste range specifically designed for children. Int Dent J 63(Suppl 2):48–56. doi:10.1111/idj.12071 CrossRefPubMed

21.

Torma H, Geijer S, Gester T, Alpholm K, Berne B, Lindberg M (2006) Variations in the mRNA expression of inflammatory mediators, markers of differentiation and lipid-metabolizing enzymes caused by sodium lauryl sulphate in cultured human keratinocytes. Toxicol in Vitro 20(4):472–479. doi:10.1016/j.tiv.2005.09.011 CrossRefPubMed

22.

Moore C, Addy M, Moran J (2008) Toothpaste detergents: a potential source of oral soft tissue damage? Int J Dent Hyg 6(3):193–198. doi:10.1111/j.1601-5037.2008.00307.x CrossRefPubMed

23.

Babich H, Babich JP (1997) Sodium lauryl sulfate and triclosan: in vitro cytotoxicity studies with gingival cells. Toxicol Lett 91(3):189–196CrossRefPubMed

Title: Dentifrices for children differentially affect cell viability in vitro
Authors: Barbara Cvikl
Adrian Lussi
Andreas Moritz
Reinhard Gruber
Publication date: 01-01-2017
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 1/2017
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-016-1813-4

At a glance: The STEP trials

Springer Medicine

Dentifrices for children differentially affect cell viability in vitro

Abstract

Objectives

Material and methods

Results

Conclusions

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Material and methods

Results

Conclusions

Clinical relevance

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