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01-01-2017 | Original Article

In vitro antibacterial and remineralizing effect of adhesive containing triazine and niobium pentoxide phosphate inverted glass

Authors: Aline Segatto Pires Altmann, Fabrício Mezzomo Collares, Vicente Castelo Branco Leitune, Rodrigo Alex Arthur, Antonio Shigueaki Takimi, Susana Maria Werner Samuel

Published in: Clinical Oral Investigations | Issue 1/2017

Abstract

Objectives

White spot lesions are still a concern for orthodontic patients. The objective of this study was to assess the remineralizing and antibacterial effect of a newly developed orthodontic adhesive.

Methods

The compounds 1,3,5-tryacryloylhexahydro-1,3,5-triazine (TAT) and phosphate invert glass containing 10 mol% of niobium pentoxide (PIG-Nb) were added at 20 and 5 wt%, respectively, to an experimental adhesive (75 wt% BisGMA, 25 wt% TEGDMA, 5 wt% fummed silica, and photo-initiator system), called TPN. A group without the addition of these compounds was used as Control and the orthodontic adhesive Transbond XT (TXT) was used for comparison. Antibacterial activity was evaluated through surface biofilm formation, mineral deposition, and degree of conversion (DC) through Raman microscopy, Knoop hardness after softening in solvent, and bracket dislodgement (BD).

Results

TPN group presented a reduction in bacterial growth when compared to Control and TXT. Mineral deposits were observed on the surface of TPN adhesive after 14 and 28 days of immersion in artificial saliva. There was an increase in DC after 28 days, whereas TPN group presented the highest DC. All groups underwent some degree of softening. No significant changes were observed in BD after 28 days of immersion in artificial saliva.

Conclusion

The newly developed orthodontic adhesive, with addition of 20 wt% TAT and 5w% PIG-Nb, exhibited antibacterial activity and was capable to induce mineral deposition on its surface in vitro.

Clinical significance

The orthodontic adhesive developed in this study with antibacterial activity and mineral deposition could be a reliable choice for brackets adhesion.

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Uysal T, Amasyali M, Ozcan S, Koyuturk AE, Sagdic D (2011) Effect of antibacterial monomer-containing adhesive on enamel demineralization around orthodontic brackets: an in-vivo study. Am J Orthod Dentofac Orthop 139:650–656. doi:10.1016/j.ajodo.2009.06.038 CrossRef

Melo MA, Wu J, Weir MD, Xu HH (2014) Novel antibacterial orthodontic cement containing quaternary ammonium monomer dimethylaminododecyl methacrylate. J Dent 42:1193–1201. doi:10.1016/j.jdent.2014.07.006 CrossRefPubMedPubMedCentral

Altmann AS, Collares FM, Ogliari FA, Samuel SM (2015) Effect of methacrylated-based antibacterial monomer on orthodontic adhesive system properties. Am J Orthod Dentofac Orthop 147:S82–S87. doi:10.1016/j.ajodo.2015.01.015 CrossRef

Bishara SE, Damon PL, Olsen ME, Jakobsen JR (1996) Effect of applying chlorhexidine antibacterial agent on the shear bond strength of orthodontic brackets. Angle Orthod 66:313–316. doi:10.1043/0003-3219(1996)066<0313:eoacaa>2.3.co;2 PubMed

Blocher S, Frankenberger R, Hellak A, Schauseil M, Roggendorf MJ, Korbmacher-Steiner HM (2015) Effect on enamel shear bond strength of adding microsilver and nanosilver particles to the primer of an orthodontic adhesive. BMC Oral Health 15:42. doi:10.1186/s12903-015-0024-8 CrossRefPubMedPubMedCentral

Malkoc S, Demir A, Sengun A, Ozer F (2005) The effect on shear bond strength of different antimicrobial agents after acid etching. Eur J Orthod 27:484–488. doi:10.1093/ejo/cji032 CrossRefPubMed

Spencer CG, Campbell PM, Buschang PH, Cai J, Honeyman AL (2009) Antimicrobial effects of zinc oxide in an orthodontic bonding agent. Angle Orthod 79:317–322. doi:10.2319/011408-19.1 CrossRefPubMed

Poosti M, Ramazanzadeh B, Zebarjad M, Javadzadeh P, Naderinasab M, Shakeri MT (2012) Shear bond strength and antibacterial effects of orthodontic composite containing TiO2 nanoparticles. Eur J Orthod 35:676–679. doi:10.1093/ejo/cjs073 CrossRefPubMed

Altmann AS, Collares FM, Leitune VC, Samuel SM (2015) The effect of antimicrobial agents on bond strength of orthodontic adhesives: ameta-analysis of in vitro studies. Orthod Craniofac Res. doi:10.1111/ocr.12100 PubMed

10.

Imazato S, Kinomoto Y, Tarumi H, Ebisu S, Tay FR (2003) Antibacterial activity and bonding characteristics of an adhesive resin containing antibacterial monomer MDPB. Dent Mater 19:313–319CrossRefPubMed

11.

Shanmugam M, Narayanan K, Chidambaranathan V, Kabilan S (2013) Synthesis, spectral characterization and antimicrobial studies of novel s-triazine derivatives. Spectrochim Acta A Mol Biomol Spectrosc 105:383–390. doi:10.1016/j.saa.2012.12.046 CrossRefPubMed

12.

Zhou C, Min J, Liu Z, Young A, Deshazer H, Gao T, Chang YT, Kallenbach NR (2008) Synthesis and biological evaluation of novel 1,3,5-triazine derivatives as antimicrobial agents. Bioorg Med Chem Lett 18:1308–1311. doi:10.1016/j.bmcl.2008.01.031 CrossRefPubMed

13.

Gange P (2015) The evolution of bonding in orthodontics. Am J Orthod Dentofac Orthop 147:S56–S63. doi:10.1016/j.ajodo.2015.01.011 CrossRef

14.

Hench LL (2006) The story of bioglass. J Mater Sci Mater Med 17:967–978. doi:10.1007/s10856-006-0432-z CrossRefPubMed

15.

Bih L, Azrour M, Manoun B, Graça MPF, Valente MA (2013) Raman spectroscopy, X-Ray, SEM, and DTA Analysis of alkali-phosphate glasses containing and Nb2O5. J Spectrosc 2013:10. doi:10.1155/2013/123519 CrossRef

16.

Alkemper J, Fuess H (1997) Devitrification of bioactive invert phosphate glasses. J Non-Cryst Solids 210:32–40. doi:10.1016/S0022-3093(96)00586-8 CrossRef

17.

Osorio R, Yamauti M, Sauro S, Watson TF, Toledano M (2013) Experimental resin cements containing bioactive fillers reduce matrix metalloproteinase-mediated dentin Collagen degradation. J Endod 38:1227–1232. doi:10.1016/j.joen.2012.05.011 CrossRef

18.

Sauro S, Watson TF, Thompson I, Banerjee A (2012) One-bottle self-etching adhesives applied to dentine air-abraded using bioactive glasses containing polyacrylic acid: an in vitro microtensile bond strength and confocal microscopy study. J Dent 40:896–905. doi:10.1016/j.jdent.2012.07.004 CrossRefPubMed

19.

Sauro S, Osorio R, Watson TF, Toledano M (2012) Therapeutic effects of novel resin bonding systems containing bioactive glasses on mineral-depleted areas within the bonded-dentine interface. J Mater Sci Mater Med 23:1521–1532. doi:10.1007/s10856-012-4606-6 CrossRefPubMed

20.

Walter G, Vogel J, Hoppe U, Hartmann P (2001) The structure of CaO–Na2O–MgO–P2O5 invert glass. J Non-Cryst Solids 296:212–223. doi:10.1016/S0022-3093(01)00912-7 CrossRef

21.

Carbonari MJ, Martinelli JR, Sene FF, König B Jr, Rogero SO (2009) Obtenção de vidros bioativos utilizados na Reparação Óssea. Revista Mackenzie De Engenharia e Computação 6-10:78–89

22.

Karlinsey RL, Hara AT, Yi K, Duhn CW (2006) Bioactivity of novel self-assembled crystalline Nb2O5 microstructures in simulated and human salivas. Biomed Mater 1:16–23. doi:10.1088/1748-6041/1/1/003 CrossRefPubMed

23.

Collares F, Portella F, da Silva FG, Semeunka S, Almeida L, Santos E, Leitune V, Samuel S (2014) Mineral deposition at dental adhesive resin containing niobium pentoxide. Appl Adhes Sci 2:1–6. doi:10.1186/s40563-014-0022-0 CrossRef

24.

Chenu S, Lebullenger R, Rocherullé J (2012) Microwave synthesis and properties of NaPO3–SnO–Nb2O5 glasses. J Mater Sci 47:4632–4639. doi:10.1007/s10853-012-6328-z CrossRef

25.

Milly H, Festy F, Watson TF, Thompson I, Banerjee A (2014) Enamel white spot lesions can remineralise using bio-active glass and polyacrylic acid-modified bio-active glass powders. J Dent 42:158–166. doi:10.1016/j.jdent.2013.11.012 CrossRefPubMed

26.

Obata A, Takahashi Y, Miyajima T, Ueda K, Narushima T, Kasuga T (2012) Effects of niobium ions released from calcium phosphate invert glasses containing Nb2O5 on osteoblast-like cell functions. ACS Appl Mater Interfaces 4:5684–5690. doi:10.1021/am301614a CrossRefPubMed

27.

Arthur RA, Waeiss RA, Hara AT, Lippert F, Eckert GE, Zero DT (2013) A defined-multispecies microbial model for studying enamel caries development. Caries Res 47:318–324CrossRefPubMed

28.

Arthur RA, Martins VB, de Oliveira CL, Leitune VCB, Collares FM, Magalhães AC, Maltz M (2015) Effect of over-the-counter fluoridated products regimens on root caries inhibition. Arch Oral Biol 60:1588–1594CrossRefPubMed

29.

Leitune VC, Collares FM, Trommer RM, Andrioli DG, Bergmann CP, Samuel SM (2013) The addition of nanostructured hydroxyapatite to an experimental adhesive resin. J Dent 41:321–327. doi:10.1016/j.jdent.2013.01.001 CrossRefPubMed

30.

Collares FM, Portella FF, Leitune VC, Samuel SM (2014) Discrepancies in degree of conversion measurements by FTIR. Brazilian Oral Res 28:9–15. doi:10.1590/s1806-83242013000600002 CrossRef

31.

Rodrigues SB, Collares, FM, Leitune, VC, Schneider LF, Ogliari FA, Petzhold CS, Samuel SM (2015) Influence of hydroxyethyl acrylamide addition to dental adhesive resin. Epub ahead Print doi:10.1016/j.dental.2015.10.005

32.

Artun J, Bergland S (1984) Clinical trials with crystal growth conditioning as an alternative to acid-etch enamel pretreatment. Am J Orthod 85:333–340CrossRefPubMed

33.

Haiges R, Belanger-Chabot G, Kaplan SM, Christe KO (2015) Synthesis and structural characterization of 3,5-dinitro-1,2,4-triazolates. Dalton Trans 44:2978–2988. doi:10.1039/c4dt03583f CrossRefPubMed

34.

Sastry SS, Rao BR (2014) Structural and optical properties of vanadium doped akaline earth lead zinc phosphate glasses. Indian J Pure Appl Phys 52:491–498

35.

Leitune VC, Takimi A, Collares FM, Santos PD, Provenzi C, Bergmann CP, Samuel SM (2013) Niobium pentoxide as a new filler for methacrylate-based root canal sealers. Int Endod J 46:205–210. doi:10.1111/j.1365-2591.2012.02107.x CrossRefPubMed

36.

Julien KC, Buschang PH, Campbell PM (2013) Prevalence of white spot lesion formation during orthodontic treatment. Angle Orthod 83:641–647. doi:10.2319/071712-584.1 CrossRefPubMed

37.

Iijima M, Muguruma T, Brantley WA, Yuasa T, Uechi J, Mizoguchi I (2010) Effect of mechanical properties of fillers on the grindability of composite resin adhesives. Am J Orthod Dentofac Orthop 138:420–426. doi:10.1016/j.ajodo.2008.08.039 CrossRef

38.

Zhou C, Weir MD, Zhang K, Deng D, Cheng L, Xu HH (2013) Synthesis of new antibacterial quaternary ammonium monomer for incorporation into CaP nanocomposite. Dent Mater 29:859–870. doi:10.1016/j.dental.2013.05.005 CrossRefPubMed

39.

Sokucu O, Siso SH, Bektas OO, Babacan H (2010) Shear bond strength comparison of a conventional and a self-etching fluoride-releasing adhesive following thermocycling. World J Orthod 11:6–10PubMed

40.

Knowles JC, Franks K, Abrahams I (2001) Investigation of the solubility and ion release in the glass system K2O-Na2O-CaO-P2O5. Biomaterials 22:3091–3096CrossRefPubMed

41.

Lopes JH, Magalhães A, Mazali IO, Bertran CA (2014) Effect of niobium oxide on the structure and properties of melt-derived bioactive glasses. J Am Ceram Soc 97:3843–3852. doi:10.1111/jace.13222 CrossRef

42.

Mehdawi I, Neel EA, Valappil SP, Palmer G, Salih V, Pratten J, Spratt DA, Young AM (2009) Development of remineralizing, antibacterial dental materials. Acta Biomater 5:2525–2539. doi:10.1016/j.actbio.2009.03.030 CrossRefPubMed

43.

Crane NJ, Popescu V, Morris MD, Steenhuis P, Ignelzi MA Jr (2006) Raman spectroscopic evidence for octacalcium phosphate and other transient mineral species deposited during intramembranous mineralization. Bone 39:434–442. doi:10.1016/j.bone.2006.02.059 CrossRefPubMed

44.

Par M, Gamulin O, Marovic D, Klaric E, Tarle Z (2015) Raman spectroscopic assessment of degree of conversion of bulk-fill resin composites - changes at 24 hours post cure. Oper Dent 40:e92–e101. doi:10.2341/14-091-l CrossRefPubMed

45.

Santerre JP, Shajii L, Leung BW (2001) Relation of dental composite formulations to their degradation and the release of hydrolyzed polymeric-resin-derived products. Crit Rev Oral Biol Med 12:136–151CrossRefPubMed

46.

Lovelh LG, Newman SM, Bowman CN (1999) The effects of light intensity, temperature, and comonomer composition on the polymerization behavior of dimethacrylate dental resins. J Dent Res 78:1469–1476. doi:10.1177/00220345990780081301 CrossRef

47.

Witzel MF, Calheiros FC, Goncalves F, Kawano Y, Braga RR (2005) Influence of photoactivation method on conversion, mechanical properties, degradation in ethanol and contraction stress of resin-based materials. J Dent 33:773–779. doi:10.1016/j.jdent.2005.02.005 CrossRefPubMed

48.

Lee JH, Um CM, Lee IB (2006) Rheological properties of resin composites according to variations in monomer and filler composition. Dent Mater 22:515–526. doi:10.1016/j.dental.2005.05.008 CrossRefPubMed

49.

Goncalves F, Kawano Y, Pfeifer C, Stansbury JW, Braga RR (2009) Influence of BisGMA, TEGDMA, and BisEMA contents on viscosity, conversion, and flexural strength of experimental resins and composites. Eur J Oral Sci 117:442–446. doi:10.1111/j.1600-0722.2009.00636.x CrossRefPubMed

50.

Tarumi H, Imazato S, Ehara A, Kato S, Ebi N, Ebisu S (1999) Post-irradiation polymerization of composites containing bis-GMA and TEGDMA. Dent Mater 15:238–242CrossRefPubMed

51.

Ferracane JL, Berge HX, Condon JR (1998) In vitro aging of dental composites in water–effect of degree of conversion, filler volume, and filler/matrix coupling. J Biomed Mater Res 42:465–472CrossRefPubMed

52.

Scougall-Vilchis RJ, Ohashi S, Yamamoto K (2009) Effects of 6 self-etching primers on shear bond strength of orthodontic brackets. Am J Orthod Dentofacial Orthop 135(424):e421–e427 discussion 424-425. doi:10.1016/j.ajodo.2008.12.005

53.

Eminkahyagil N, Arman A, Cetinsahin A, Karabulut E (2006) Effect of resin-removal methods on enamel and shear bond strength of rebonded brackets. Angle Orthod 76:314–321. doi:10.1043/0003-3219(2006)076[0314:eormoe]2.0.co;2 PubMed

54.

Retamoso LB, Collares FM, Ferreira ES, Samuel SM (2009) Shear bond strength of metallic brackets: influence of saliva contamination. J Appl Oral Sci 17:190–194CrossRefPubMedPubMedCentral

55.

Dawes C (2003) What is the critical pH and why does a tooth dissolve in acid? J Can Dent Assoc 69:722–724PubMed

56.

de Gee AJ, Wendt SL, Werner A, Davidson CL (1996) Influence of enzymes and plaque acids on in vitro wear of dental composites. Biomaterials 17:1327–1332CrossRefPubMed

57.

Milly H, Festy F, Andiappan M, Watson TF, Thompson I, Banerjee A (2015) surface pre-conditioning with bioactive glass air-abrasion can enhance enamel white spot lesion remineralization. Dent mater 31:522–533. doi:10.1016/j.dental.2015.02.002 CrossRefPubMed

Title: In vitro antibacterial and remineralizing effect of adhesive containing triazine and niobium pentoxide phosphate inverted glass
Authors: Aline Segatto Pires Altmann
Fabrício Mezzomo Collares
Vicente Castelo Branco Leitune
Rodrigo Alex Arthur
Antonio Shigueaki Takimi
Susana Maria Werner Samuel
Publication date: 01-01-2017
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 1/2017
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-016-1754-y

At a glance: The STEP trials

Springer Medicine

In vitro antibacterial and remineralizing effect of adhesive containing triazine and niobium pentoxide phosphate inverted glass

Abstract

Objectives

Methods

Results

Conclusion

Clinical significance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Methods

Results

Conclusion

Clinical significance

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