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Clinical Oral Investigations
Published in: Clinical Oral Investigations 3/2021

01-03-2021 | Denosumab | Original Article

Effect of root surface treatment with denusomab after delayed tooth replantation

Authors: Léa Assed Bezerra da Silva, Daniele Lucca Longo, Maria Bernadete Sasso Stuani, Alexandra Mussolino de Queiroz, Raquel Assed Bezerra da Silva, Paulo Nelson-Filho, Heloisa Aparecida Orsini Vieira, Carolina Maschietto Pucinelli, Francisco Wanderley Garcia Paula-Silva

Published in: Clinical Oral Investigations | Issue 3/2021

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Abstract

Objectives

To evaluate denosumab, a human monoclonal antibody that mimics the effects of osteoprotegerin in bone metabolism, as a topical treatment of root surface to be used prior to delayed tooth replantation.

Materials and methods

Thirty-six rats’ right incisors were used. Teeth were extracted and divided into: delayed replantation without root surface treatment (control); delayed replantation with root surface treatment with denosumab 60 mg/mL and 30 mg/mL, respectively, for 10 min both experimentals groups. After that, the root canals were filled with calcium hydroxide and replanted. After 15 and 60 days, the animals were euthanized, and the samples were collected and processed for microscopic analysis. Histological sections were performed, and stained with HE to describe the dental characteristics, measure ankylosis, replacement resorption, and dental resorption by conventional microscopy. Also, was performed Brown & Brenn staining and immunohistochemistry for RANKL, OPG, and periostin.

Results

Denosumab 60 mg/mL reducted ankylosis (p < 0.0001), replacement resorption (p < 0.0001), and tooth resorption, 60 days after replantation, compared to untreated replanted teeth (p < 0.005). Lower bacterial contamination in root surface in the denosumab treatment groups was found, regardless of the concentration used (p < 0.001). Also, denosumab treatment inhibited the expression of RANKL without modulating OPG. Periostin was observed in periodontal ligament of replanted tooth, although this labelling was absent in the ankylosis areas, in both experimental periods.

Conclusion

Treatment of the root surface with denosumab at 60 mg/mL of rat teeth before delayed replantation reduced dental root resorption compared with the untreated teeth after 60 days.

Clinical relevance

Survival of a replanted tooth has been a challenge in clinical practice. The use of a medication, such as denosumab, to limit dental root resorption represents an important therapeutical approach.
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Metadata
Title
Effect of root surface treatment with denusomab after delayed tooth replantation
Authors
Léa Assed Bezerra da Silva
Daniele Lucca Longo
Maria Bernadete Sasso Stuani
Alexandra Mussolino de Queiroz
Raquel Assed Bezerra da Silva
Paulo Nelson-Filho
Heloisa Aparecida Orsini Vieira
Carolina Maschietto Pucinelli
Francisco Wanderley Garcia Paula-Silva
Publication date
01-03-2021
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 3/2021
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-020-03430-1

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