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01-03-2021 | Dengue Virus | Original Article

Molecular characterization of structural protein genes of dengue virus serotype 1 epidemic in Yunnan, Southwest China, in 2018

Authors: Qingping Lan, Yun Shu, Linhao Li, Xiyun Shan, Dehong Ma, Tingting Li, Xiaodan Wang, Yue Pan, Junying Chen, Juan Zhang, Pinghua Liu, Qiangming Sun

Published in: Archives of Virology | Issue 3/2021

Abstract

A dengue virus serotype 1 (DENV-1) epidemic occurred from October to December 2018 in Xishuangbanna, Yunnan, Southwest China, neighboring Myanmar, Laos, and Vietnam. In this study, we investigated the molecular characteristics, evolution, and potential source of DENV from Xishuangbanna. The C (capsid), prM (premembrane), and E (envelope) genes of DENV isolated from 87 serum samples obtained from local patients were amplified and sequenced, and the sequences were evaluated by identification of mutations, phylogenetic and homologous recombination analysis, and secondary structure prediction. Phylogenetic analysis showed that all of the epidemic DENV strains from Xishuangbanna could be grouped in a branch with DENV-1 isolates, and were most similar to the Fujian 2005 (China, DQ193572) and Singapore 2016 (MF314188) strains. When compared with DENV-1SS (the standard strain), there were 31 non-synonymous mutations, but no obvious homologous recombination signal was found. Secondary structure prediction showed that some changes had occurred in a helical region in proteins of the MN123849 and MN123854 strains, but there were few changes in the disordered region. This study reveals the molecular characteristics of the structural genes of the Xishuangbanna epidemic strains in 2018 and provides a reference for molecular epidemiology, infection, and pathogenicity research and vaccine development.

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Title: Molecular characterization of structural protein genes of dengue virus serotype 1 epidemic in Yunnan, Southwest China, in 2018
Authors: Qingping Lan
Yun Shu
Linhao Li
Xiyun Shan
Dehong Ma
Tingting Li
Xiaodan Wang
Yue Pan
Junying Chen
Juan Zhang
Pinghua Liu
Qiangming Sun
Publication date: 01-03-2021
Publisher: Springer Vienna
Keyword: Dengue Virus
Published in: Archives of Virology / Issue 3/2021
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04942-7

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