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Virology Journal
Published in: Virology Journal 1/2015

Open Access 01-12-2015 | Research

DAXX modulates human papillomavirus early gene expression and genome replication in U2OS cells

Authors: Piia Kivipõld, Liisi Võsa, Mart Ustav, Reet Kurg

Published in: Virology Journal | Issue 1/2015

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Abstract

Background

The human papillomavirus (HPV) genomes can replicate, and are maintained as autonomously replicating extrachromosomal plasmids in human U2OS cells. Previous studies have shown that HPV genomes are transcriptionally active in U2OS cells and can express the viral early proteins required for initiation and establishment of HPV replication. In the present work, we have examined the involvement of cellular DAXX protein in HPV replication in U2OS cells.

Methods

We have used indirect immunofluorescence and FISH analysis in order to study HPV replication compartments in U2OS cells. In addition, we have used siRNA knock-down for examining the effect of the DAXX protein on HPV replication and transcription in U2OS cells.

Results

We show that a portion of HPV replication foci are partially co-localized with components of ND10, cellular DAXX and PML proteins. In addition, we demonstrate that the knock-down of the cellular DAXX protein modulates the HPV genome replication and transcription in U2OS cells – papillomavirus replication is reduced in the absence of this component of ND10.

Conclusions

The DAXX protein modulates the early gene expression and the transient replication of HPV genomes in U2OS cells.
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Metadata
Title
DAXX modulates human papillomavirus early gene expression and genome replication in U2OS cells
Authors
Piia Kivipõld
Liisi Võsa
Mart Ustav
Reet Kurg
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2015
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-015-0335-z

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