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Comparative Hepatology
Published in: Comparative Hepatology 1/2004

Open Access 01-01-2004 | Proceedings

Cytotoxic reactions of CC531s towards liver sinusoidal endothelial cells: a microscopical study

Authors: Katrien Vekemans, Maarten Timmers, David Vermijlen, Ronald De Zanger, Eddie Wisse, Filip Braet

Published in: Comparative Hepatology | Special Issue 1/2004

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Excerpt

Colorectal cancer cells can induce apoptosis in cells of various tissues [1]. Apoptosis can be induced by a number of factors such as Fas inducing apoptosis through the Fas/FasL pathway; other factors involve the TRAIL pathway and TNF. It is known that metastasizing colon cancer cells express more FasL then primary carcinoma cells [2]. We investigated whether a rat colon carcinoma cell line CC531s could induce apoptosis in liver sinusoidal endothelial cells (LSECs). LSECs and CC531s were co-cultured for 18 hrs and cells were visualized by SEM and TEM. Apoptosis was visualized by markers such as Hoechst and Propidium iodide. Furthermore, cells were recorded by time lapse video microscopy with and without an antagonistic antibody for FasL. …
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Braet F, De Zanger R, Sasaoki T, Baekeland M, Janssens P, Smedsr–d B, Wisse E: Assessment of a method of isolation, purification and cultivation of rat liver sinusoidal endothelial cells. Lab Invest. 1994, 70: 944-952.PubMed
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Braet F, De Zanger R, Wisse E: Drying cells for SEM, AFM and TEM by hexamethyldisilazane: a study on hepatic endothelial cells. J Microsc. 1997, 186: 84-87. 10.1046/j.1365-2818.1997.1940755.x.CrossRefPubMed
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Metadata
Title
Cytotoxic reactions of CC531s towards liver sinusoidal endothelial cells: a microscopical study
Authors
Katrien Vekemans
Maarten Timmers
David Vermijlen
Ronald De Zanger
Eddie Wisse
Filip Braet
Publication date
01-01-2004
Publisher
BioMed Central
Published in
Comparative Hepatology / Issue Special Issue 1/2004
Electronic ISSN: 1476-5926
DOI
https://doi.org/10.1186/1476-5926-2-S1-S49

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