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01-07-2011 | Short Communication

Cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic differentiation of human dental pulp cells

Authors: Masanori Anpo, Kumiko Shirayama, Takeki Tsutsui

Published in: Odontology | Issue 2/2011

Abstract

The cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic differentiation of human dental pulp cells such as collagen synthesis and the expression of two osteogenesis-related genes, alkaline phosphatase (ALP) and bone sialoprotein (BSP), was studied using human dental pulp cells (D824 cells). Cellular growth and survival were decreased by treatment of cells with eugenol in a concentration-dependent manner. The incorporation rate of [³H] proline into the acid-insoluble fraction and the synthesis of type I–V collagens were also reduced by treatment of cells with eugenol in a concentration-dependent fashion. The mRNA expression of ALP was scarcely affected in cells exposed to eugenol, whereas the mRNA and protein expression of BSP was down-regulated depending on the concentrations of eugenol. The results suggest that because collagen synthesis and BSP expression play a critical role in hard tissue formation, eugenol used for endodontic treatment may give rise to cytotoxic effects to the normal function of stem cells reported to exist in human dental pulp tissue and periodontal ligament.

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Title: Cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic differentiation of human dental pulp cells
Authors: Masanori Anpo
Kumiko Shirayama
Takeki Tsutsui
Publication date: 01-07-2011
Publisher: Springer Japan
Published in: Odontology / Issue 2/2011
Print ISSN: 1618-1247
Electronic ISSN: 1618-1255
DOI: https://doi.org/10.1007/s10266-011-0009-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic differentiation of human dental pulp cells

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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