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01-03-2021 | Cytokines | Original Article

Immunological profile of periapical endodontic infection in patients undergoing haematopoietic transplantation

Authors: Julia Mourão Braga Diniz, Marcela Carvalho Espaladori, Maria Elisa e Souza Silva, Luciana Carla Neves de Brito, Leda Quercia Vieira, Antônio Paulino Ribeiro Sobrinho

Published in: Clinical Oral Investigations | Issue 3/2021

Abstract

Objectives

To evaluate the mRNA expression levels of cytokines interferon-γ, tumour necrosis factor-α, interleukin IL-1β, IL-10, and the chemokine CCL2/MCP-1, CCL4, and CXCR4 in the periapical interstitial fluid from root canal infections before and after bacterial load reduction in patients undergoing haematopoietic stem cell transplantation (HSCT).

Materials and methods

The case group was composed of 10 patients undergoing HSCT, and our control group included 10 healthy patients. Clinical samples were taken from teeth with pulp necrosis. Three paper points were placed in the RCS and maintained for 2 min for microbial evaluation before cleaning and shaping procedures. After cleaning and drying the canal, three paper points were introduced into the root canal, passing passively through the root apex (2 mm) into the periapical tissues for 1 min. Samples were collected immediately after root canal cleaning and 7 days later (restrained root canal bacterial load) to characterize gene expression using real-time PCR.

Results

The results showed significantly reduction in the microbial load on day 7. An increased expression level of TNF-α and IFN-γ on day 7 in control and case groups was observed (p < 0.05). The mRNA levels of IL-1β and IL-10 in the pre-HSCT group increased in the samples from day 7 (p < 0.05). The chemokine CCL-2/MCP-1 was not detected in pre-HSCT group. Chemokine receptor CXCR4 levels increased in samples obtained from the day 7 in the control group (p < 0.05).

Conclusions

Individuals undergoing HSTC presented similar cytokine and chemokine mRNA expression compared with healthy individuals. However, it was observed the total absence of mRNA MCP-1/CCL2 expression in those individuals undergoing HSCT.

Clinical relevance

Patients undergoing HSCT are at higher risk of infection. No study has analysed the periapical immune responses to root canal infections in HSCT individuals.

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Title: Immunological profile of periapical endodontic infection in patients undergoing haematopoietic transplantation
Authors: Julia Mourão Braga Diniz
Marcela Carvalho Espaladori
Maria Elisa e Souza Silva
Luciana Carla Neves de Brito
Leda Quercia Vieira
Antônio Paulino Ribeiro Sobrinho
Publication date: 01-03-2021
Publisher: Springer Berlin Heidelberg
Keywords: Cytokines
Stem Cell Transplantation
Stem Cell Transplantion
Stem Cell Transplantation
Published in: Clinical Oral Investigations / Issue 3/2021
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-020-03448-5

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Springer Medicine

Immunological profile of periapical endodontic infection in patients undergoing haematopoietic transplantation

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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