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Journal of Inflammation
Published in: Journal of Inflammation 1/2013

Open Access 01-12-2013 | Research

Cross-linking of IgGs bound on circulating neutrophils leads to an activation of endothelial cells: possible role of rheumatoid factors in rheumatoid arthritis-associated vascular dysfunction

Authors: Emmanuelle Rollet-Labelle, Myriam Vaillancourt, Louis Marois, Marianna M Newkirk, Patrice E Poubelle, Paul H Naccache

Published in: Journal of Inflammation | Issue 1/2013

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Abstract

Background

Rheumatoid arthritis is characterized by the presence of circulating auto-antibodies, including rheumatoid factors, which recognize the Fc portion of IgGs. The neutrophil is the most abundant circulating leukocyte and it expresses high levels of FcγRs on its surface. The aim of the present study was to examine the capacity of circulating human neutrophils to be activated by rheumatoid factors and the consequences of these events on endothelium.

Methods

Neutrophil-bound IgGs were cross-linked with anti-human IgGs to mimick the presence of circulating rheumatoid factors and FcγRs-dependent signalling events and functions were examined. The IgG and IgM composition of rheumatoid factors isolated from the serum of RA patients was characterized. Adhesion of neutrophils to endothelial cells was quantified in response to the addition of rheumatoid factors.

Results

Cross-linking of IgGs bound on neutrophils leads to FcγRs-dependent tyrosine phosphorylation, mobilisation of intracellular calcium and the extracellular release of superoxide anions and lysozyme. Incubation of endothelial cells with the supernatant of activated neutrophils increases ICAM-1 expression and IL-8 production by endothelial cells. Finally, rheumatoid factors enhance neutrophil adhesion to endothelial cells.

Conclusions

Our results show that activation of neutrophils’ FcγRs by rheumatoid factors could participate in rheumatoid arthritis-associated vascular damage.
Appendix
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Metadata
Title
Cross-linking of IgGs bound on circulating neutrophils leads to an activation of endothelial cells: possible role of rheumatoid factors in rheumatoid arthritis-associated vascular dysfunction
Authors
Emmanuelle Rollet-Labelle
Myriam Vaillancourt
Louis Marois
Marianna M Newkirk
Patrice E Poubelle
Paul H Naccache
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Journal of Inflammation / Issue 1/2013
Electronic ISSN: 1476-9255
DOI
https://doi.org/10.1186/1476-9255-10-27

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