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World Journal of Surgical Oncology

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Open Access 01-12-2013 | Research

Cortactin is a sensitive biomarker relative to the poor prognosis of human hepatocellular carcinoma

Authors: Gang Zhao, Zi-ming Huang, Ya-Lin Kong, Dong-Qing Wen, Yu Li, Li Ren, Hong-Yi Zhang

Published in: World Journal of Surgical Oncology | Issue 1/2013

Abstract

Background

Cortactin is an important regulator involved in invasion and migration of hepatocellular carcinoma (HCC). The aim of this study was to elucidate the forecasting role of cortactin in resectable HCCs.

Methods

We compared the invasiveness and motility among liver epithelial cell line and HCC cell lines by using Transwell assay and wound healing assay. We further investigated the CTTN mRNA expression by real-time PCR. Next, 91 HCC and 20 normal liver tissue samples were detected by IHC and real-time PCR. Finally, we analyzed the clinicopathologic features and survival time of the HCC cases.

Results

We identified that HepG2, LM3, and SK-Hep-1 had more invasiveness and motility (P <0.05). Compared with liver epithelial cell line, CTTN expression was higher in LM3, HepG2, and MHCC97-L (P <0.01) and lower in SK-Hep-1 (P <0.05). IHC examination showed cortactin expression was closely relative to TNM stage (AJCC/UICC), cancer embolus, and metastasis (P <0.01). Cortactin overexpression indicated a longer survival time of 52 ± 8.62 months and low expression of a shorter survival time of 20 ± 4.95 months (P <0.01). Cortactin examination has more predictive power in patients with Child-Pugh grade A and BCLC stage 0-B.

Conclusions

Overexpression of cortactin is closely associated with poor human HCCs prognosis that caused by cancer embolus and metastasis. Cortactin and CTTN should be used for differentiating varieties of survival for patients after HCC resection.

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Title: Cortactin is a sensitive biomarker relative to the poor prognosis of human hepatocellular carcinoma
Authors: Gang Zhao
Zi-ming Huang
Ya-Lin Kong
Dong-Qing Wen
Yu Li
Li Ren
Hong-Yi Zhang
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: World Journal of Surgical Oncology / Issue 1/2013
Electronic ISSN: 1477-7819
DOI: https://doi.org/10.1186/1477-7819-11-74

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Cortactin is a sensitive biomarker relative to the poor prognosis of human hepatocellular carcinoma

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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