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01-02-2015 | Short Communication

Correlation Between Attenuation of Protein Disulfide Isomerase Activity Through S-Mercuration and Neurotoxicity Induced by Methylmercury

Authors: Kento Makino, Kosaku Okuda, Eisuke Sugino, Tadashi Nishiya, Takashi Toyama, Takao Iwawaki, Masatake Fujimura, Yoshito Kumagai, Takashi Uehara

Published in: Neurotoxicity Research | Issue 2/2015

Abstract

Methylmercury (MeHg), an environmental pollutant, causes neuronal death via endoplasmic reticulum (ER) stress; however, the precise mechanism is not fully understood. The aim of this study was to elucidate the possible mechanism of MeHg-induced neurotoxicity. Treatment with MeHg resulted in a loss of cell viability in a concentration-dependent manner accompanying the expression of ER stress marker genes in human neuroblastoma SH-SY5Y cells. We next attempted to identify a target protein for MeHg in the ER. MeHg covalently modified protein disulfide isomerase (PDI), which is important for disulfide bond formation in nascent proteins in the ER lumen. S-Nitrosylation of the catalytic domains of PDI by nitric oxide was attenuated up to 50 % by a MeHg challenge in cells. The MeHg-modified C-terminal catalytic domain in PDI was detected by MALDI-TOF/MS. Furthermore, treatment with MeHg significantly attenuated the enzymatic activity of PDI. Taken together, these observations suggest that MeHg results in ER stress and following the unfolded protein response pathway via ER dysfunction due to S-mercuration of the C-terminus of PDI.

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Title: Correlation Between Attenuation of Protein Disulfide Isomerase Activity Through S-Mercuration and Neurotoxicity Induced by Methylmercury
Authors: Kento Makino
Kosaku Okuda
Eisuke Sugino
Tadashi Nishiya
Takashi Toyama
Takao Iwawaki
Masatake Fujimura
Yoshito Kumagai
Takashi Uehara
Publication date: 01-02-2015
Publisher: Springer US
Published in: Neurotoxicity Research / Issue 2/2015
Print ISSN: 1029-8428
Electronic ISSN: 1476-3524
DOI: https://doi.org/10.1007/s12640-014-9494-8

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Correlation Between Attenuation of Protein Disulfide Isomerase Activity Through S-Mercuration and Neurotoxicity Induced by Methylmercury

Abstract

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Abstract

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