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Archives of Virology
Published in: Archives of Virology 10/2016

01-10-2016 | Brief Report

Comparison of the immunogenicity of two inactivated recombinant rabies viruses overexpressing the glycoprotein

Authors: Muhammad Tariq Navid, Yingying Li, Ming Zhou, Min Cui, Zhen F. Fu, Lijun Tang, Ling Zhao

Published in: Archives of Virology | Issue 10/2016

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Abstract

Two recombinant rabies viruses overexpressing their glycoprotein (G) were compared in this study, with the overexpressed G inserted between P and M genes (named LBNSE-PM-G), and between the G and L genes (named LBNSE-GL-G), respectively. LBNSE-PM-G produced more G protein and induced stronger apoptosis than LBNSE-GL-G in infected cells, while the amount of virion-incorporated G in LBNSE-PM-G was less than in LBNSE-GL-G. Mice immunized with inactivated LBNSE-PM-G produced lower titers of virus-neutralizing antibody, and this recombinant conferred worse protection than LBNSE-GL-G. Our results suggest that over expressed G gene inserted between G and L, but not between P and M, enhanced the immunogenicity when used as an inactivated rabies vaccine.
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Metadata
Title
Comparison of the immunogenicity of two inactivated recombinant rabies viruses overexpressing the glycoprotein
Authors
Muhammad Tariq Navid
Yingying Li
Ming Zhou
Min Cui
Zhen F. Fu
Lijun Tang
Ling Zhao
Publication date
01-10-2016
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 10/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-016-2967-8

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