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EJNMMI Research
Published in: EJNMMI Research 1/2016

Open Access 01-12-2016 | Original research

Comparison of LDPI to SPECT perfusion imaging using 99mTc-sestamibi and 99mTc-pyrophosphate in a murine ischemic hind limb model of neovascularization

Authors: Geert Hendrikx, Mark H. Vries, Matthias Bauwens, Marijke De Saint-Hubert, Allard Wagenaar, Joël Guillaume, Levinia Boonen, Mark J. Post, Felix M. Mottaghy

Published in: EJNMMI Research | Issue 1/2016

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Abstract

Background

We aimed to determine the accuracy of laser Doppler perfusion imaging (LDPI) in an animal model for hind limb ischemia.

Methods

We used a murine (C57Bl/6 mice) ischemic hind limb model in which we compared LDPI with the clinically used 99mTc-sestamibi SPECT perfusion imaging (n = 7). In addition, we used the SPECT tracer 99mTc-pyrophosphate (99mTc-PyP) to image muscular damage (n = 6).

Results

LDPI indicated a quick and prominent decrease in perfusion immediately after ligation, subsequently recovering to 21.9 and 25.2 % 14 days later in the 99mTc-sestamibi and 99mTc-PyP group, respectively. 99mTc-sestamibi SPECT scans also showed a quick decrease in perfusion. However, nearly full recovery was reached 7 days post ligation. Muscular damage, indicated by the uptake of 99mTc-PyP, was highest at day 3 and recovered to baseline levels at day 14 post ligation. Postmortem histology supported these findings, as a significantly increased collateral diameter was found 7 and 14 days after ligation and peak macrophage infiltration and TUNEL positivity was found on day 3 after ligation.

Conclusions

Here, we indicate that LDPI strongly underestimates perfusion recovery in a hind limb model for profound ischemia.
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Metadata
Title
Comparison of LDPI to SPECT perfusion imaging using 99mTc-sestamibi and 99mTc-pyrophosphate in a murine ischemic hind limb model of neovascularization
Authors
Geert Hendrikx
Mark H. Vries
Matthias Bauwens
Marijke De Saint-Hubert
Allard Wagenaar
Joël Guillaume
Levinia Boonen
Mark J. Post
Felix M. Mottaghy
Publication date
01-12-2016
Publisher
Springer Berlin Heidelberg
Published in
EJNMMI Research / Issue 1/2016
Electronic ISSN: 2191-219X
DOI
https://doi.org/10.1186/s13550-016-0199-2

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