Published in:
Open Access
01-12-2013 | Genome Announcement
Comparing the genomes of Helicobacter pylori clinical strain UM032 and Mice-adapted derivatives
Authors:
Yalda Khosravi, Vellaya Rehvathy, Wei Yee Wee, Susana Wang, Primo Baybayan, Siddarth Singh, Meredith Ashby, Junxian Ong, Arlaine Anne Amoyo, Shih Wee Seow, Siew Woh Choo, Tim Perkins, Eng Guan Chua, Alfred Tay, Barry James Marshall, Mun Fai Loke, Khean Lee Goh, Sven Pettersson, Jamuna Vadivelu
Published in:
Gut Pathogens
|
Issue 1/2013
Login to get access
Abstract
Background
Helicobacter pylori is a Gram-negative bacterium that persistently infects the human stomach inducing chronic inflammation. The exact mechanisms of pathogenesis are still not completely understood. Although not a natural host for H. pylori, mouse infection models play an important role in establishing the immunology and pathogenicity of H. pylori. In this study, for the first time, the genome sequences of clinical H. pylori strain UM032 and mice-adapted derivatives, 298 and 299, were sequenced using the PacBio Single Molecule, Real-Time (SMRT) technology.
Result
Here, we described the single contig which was achieved for UM032 (1,599,441 bp), 298 (1,604,216 bp) and 299 (1,601,149 bp). Preliminary analysis suggested that methylation of H. pylori genome through its restriction modification system may be determinative of its host specificity and adaptation.
Conclusion
Availability of these genomic sequences will aid in enhancing our current level of understanding the host specificity of H. pylori.