Top

BMC Complementary Medicine and Therapies

Published in:

Open Access 01-12-2021 | Research article

Co-electrospun nanofibrous mats loaded with bitter gourd (Momordica charantia) extract as the wound dressing materials: in vitro and in vivo study

Authors: Mohammad Saeid Salami, Gholamreza Bahrami, Elham Arkan, Zhila Izadi, Shahram Miraghaee, Hadi Samadian

Published in: BMC Complementary Medicine and Therapies | Issue 1/2021

Abstract

Background

Interactive dressings are innovatively designed to interact with the wound surface and alter the wound environment to promote wound healing. In the current study, we integrated the physicochemical properties of Poly (caprolactone)/ Poly (vinyl alcohol)/Collagen (PCL/PVA/Col) nanofibers with the biological activities of Momordica charantia pulp extract to develop an efficient wound dressing. The electrospinning method was applied to fabricate the nanofibers, and the prepared wound dressings were thoroughly characterized.

Results

SEM imaging showed that the nanofibers were uniform, straight, without any beds with a diameter in the range of 260 to 480 nm. Increasing the concentration of the extract increased the diameter of the nanofibers and also the wettability characteristics while reduced the ultimate tensile strength from 4.37 ± 0.90 MPa for PCL/PVA/Col to 1.62 ± 0.50 MPa for PCL/PVA/Col/Ex 10% (p < 0.05). The in vivo studies showed that the application of the wound dressings significantly enhanced the healing process and the highest wound closure, 94.01 ± 8.12%, was obtained by PCL/PVA/Col/Ex 10% nanofibers (p < 0.05).

Conclusion

The incorporation of the extract had no significant effects on nanofibers’ porosity, water vapor permeability, and swelling characteristics. The in vitro evaluations showed that the fabricated nanofibers were hemocompatible, cytocompatible, and prevent bacterial penetration through the dressing. These findings implied that the PCL/PVA/Col/Ex nanofibers can be applied as the wound dressing materials.

Monfared GS, Ertl P, Rothbauer M. An on-chip wound healing assay fabricated by xurography for evaluation of dermal fibroblast cell migration and wound closure. Sci Rep. 2020;10(1):1–14.CrossRef

Eskandarinia A, Kefayat A, Agheb M, Rafienia M, Baghbadorani MA, Navid S, et al. A novel bilayer wound dressing composed of a dense polyurethane/Propolis membrane and a biodegradable Polycaprolactone/gelatin Nanofibrous scaffold. Sci Rep. 2020;10(1):1–15.CrossRef

Ullah A, Ullah S, Khan MQ, Hashmi M, Nam PD, Kato Y, et al. Manuka honey incorporated cellulose acetate nanofibrous mats: fabrication and in vitro evaluation as a potential wound dressing. Int J Biol Macromol. 2020;155:479–89. https://doi.org/10.1016/j.ijbiomac.2020.03.237.CrossRefPubMed

Ambekar RS, Kandasubramanian B. Advancements in nanofibers for wound dressing: a review. Eur Polym J. 2019;117:304–36. https://doi.org/10.1016/j.eurpolymj.2019.05.020.CrossRef

Obagi Z, Damiani G, Grada A, Falanga V. Principles of wound dressings: a review. Surgical technology international. 2019;35:50–7.

Soleimani K, Arkan E, Derakhshankhah H, Haghshenas B, Jahanban-Esfahlan R, Jaymand M. A novel bioreducible and pH-responsive magnetic nanohydrogel based on β-cyclodextrin for chemo/hyperthermia therapy of cancer. Carbohydr Polym. 2020;252:117229.CrossRef

Samadian H, Mobasheri H, Hasanpour S, Ai J, Azamie M, Faridi-Majidi R. Electro-conductive carbon nanofibers as the promising interfacial biomaterials for bone tissue engineering. J Mol Liq. 2020;298:112021. https://doi.org/10.1016/j.molliq.2019.112021.CrossRef

Ullah S, Ullah A, Lee J, Jeong Y, Hashmi M, Zhu C, et al. Reusability comparison of melt-blown vs nanofiber face mask filters for use in the coronavirus pandemic. ACS Applied Nano Materials. 2020;3(7):7231–41. https://doi.org/10.1021/acsanm.0c01562.CrossRef

Hashmi M, Ullah S, Kim IS. Copper oxide (CuO) loaded polyacrylonitrile (PAN) nanofiber membranes for antimicrobial breath mask applications. Curr Res Biotechnol. 2019;1:1–10. https://doi.org/10.1016/j.crbiot.2019.07.001.CrossRef

10.

Fazlalizadeh F, Massoumi B, Banaei A, Jaymand M. A thermal-responsive Y-shaped Miktoarm Amphiphilic block copolymer composed of poly (ε-caprolactone) and poly (N-isopropylacrylamide) as a Nano-micellar carrier for anti-cancer drugs. Polym Sci Ser B. 2020;62:1–10.

11.

Massoumi B, Jafarpour P, Jaymand M, Entezami AA. Functionalized multiwalled carbon nanotubes as reinforcing agents for poly (vinyl alcohol) and poly (vinyl alcohol)/starch nanocomposites: synthesis, characterization and properties. Polym Int. 2015;64(5):689–95. https://doi.org/10.1002/pi.4867.CrossRef

12.

Hashmi M, Ullah S, Ullah A, Khan MQ, Hussain N, Khatri M, et al. An optimistic approach “from hydrophobic to super hydrophilic nanofibers” for enhanced absorption properties. Polym Test. 2020;90:106683. https://doi.org/10.1016/j.polymertesting.2020.106683.CrossRef

13.

Hashmi M, Ullah S, Ullah A, Akmal M, Saito Y, Hussain N, et al. Optimized loading of carboxymethyl cellulose (CMC) in tri-component electrospun nanofibers having uniform morphology. Polymers. 2020;12(11):2524. https://doi.org/10.3390/polym12112524.CrossRefPubMedCentral

14.

Ullah S, Hashmi M, Hussain N, Ullah A, Sarwar MN, Saito Y, et al. Stabilized nanofibers of polyvinyl alcohol (PVA) crosslinked by unique method for efficient removal of heavy metal ions. J Water Process Eng. 2020;33:101111. https://doi.org/10.1016/j.jwpe.2019.101111.CrossRef

15.

Samadian H, Maleki H, Allahyari Z, Jaymand M. Natural polymers-based light-induced hydrogels: promising biomaterials for biomedical applications. Coord Chem Rev. 2020;420:213432. https://doi.org/10.1016/j.ccr.2020.213432.CrossRef

16.

Samadian H, Maleki H, Fathollahi A, Salehi M, Gholizadeh S, Derakhshankhah H, et al. Naturally occurring biological macromolecules-based hydrogels: potential biomaterials for peripheral nerve regeneration. Int J Biol Macromol. 2020;154:795–817. https://doi.org/10.1016/j.ijbiomac.2020.03.155.CrossRefPubMed

17.

Khan MF, Abutaha N, Nasr FA, Alqahtani AS, Noman OM, Wadaan MA. Bitter gourd (Momordica charantia) possess developmental toxicity as revealed by screening the seeds and fruit extracts in zebrafish embryos. BMC Complement Altern Med. 2019;19(1):1–13.CrossRef

18.

Sagkan RI. An in vitro study on the risk of non-allergic type-I like hypersensitivity to Momordica charantia. BMC Complement Altern Med. 2013;13(1):1–5.CrossRef

19.

Fernandes NP, Lagishetty CV, Panda VS, Naik SR. An experimental evaluation of the antidiabetic and antilipidemic properties of a standardized Momordica charantia fruit extract. BMC Complement Altern Med. 2007;7(1):29. https://doi.org/10.1186/1472-6882-7-29.CrossRefPubMedPubMedCentral

20.

Grover J, Yadav S. Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharmacol. 2004;93(1):123–32. https://doi.org/10.1016/j.jep.2004.03.035.CrossRefPubMed

21.

Panda BC, Mondal S, Devi KSP, Maiti TK, Khatua S, Acharya K, et al. Pectic polysaccharide from the green fruits of Momordica charantia (Karela): structural characterization and study of immunoenhancing and antioxidant properties. Carbohydr Res. 2015;401:24–31. https://doi.org/10.1016/j.carres.2014.10.015.CrossRefPubMed

22.

Raish M. Momordica charantia polysaccharides ameliorate oxidative stress, hyperlipidemia, inflammation, and apoptosis during myocardial infarction by inhibiting the NF-κB signaling pathway. Int J Biol Macromol. 2017;97:544–51. https://doi.org/10.1016/j.ijbiomac.2017.01.074.CrossRefPubMed

23.

Raish M, Ahmad A, Jan BL, Alkharfy KM, Ansari MA, Mohsin K, et al. Momordica charantia polysaccharides mitigate the progression of STZ induced diabetic nephropathy in rats. Int J Biol Macromol. 2016;91:394–9. https://doi.org/10.1016/j.ijbiomac.2016.05.090.CrossRefPubMed

24.

Chaturvedi P. Antidiabetic potentials of Momordica charantia: multiple mechanisms behind the effects. J Med Food. 2012;15(2):101–7. https://doi.org/10.1089/jmf.2010.0258.CrossRefPubMed

25.

Hashmi M, Ullah S, Kim IS. Electrospun Momordica charantia incorporated polyvinyl alcohol (PVA) nanofibers for antibacterial applications. Mater Today Commun. 2020;24:101161. https://doi.org/10.1016/j.mtcomm.2020.101161.CrossRef

26.

Cui H, Yang X, Abdel-Samie MA, Lin L. Cold plasma treated phlorotannin/Momordica charantia polysaccharide nanofiber for active food packaging. Carbohydr Polym. 2020;239:116214. https://doi.org/10.1016/j.carbpol.2020.116214.CrossRefPubMed

27.

Park SH, Yi Y-S, Kim M-Y, Cho JY. Antioxidative and antimelanogenesis effect of momordica charantia methanol extract. Evid Based Complement Alternat Med. 2019;2019:1–11. https://doi.org/10.1155/2019/5091534.CrossRef

28.

Suryamathi M, Ruba C, Viswanathamurthi P, Balasubramanian V, Perumal P. Tridax procumbens extract loaded electrospun PCL nanofibers: a novel wound dressing material. Macromol Res. 2019;27(1):55–60. https://doi.org/10.1007/s13233-019-7022-7.CrossRef

29.

Samadian H, Zamiri S, Ehterami A, Farzamfar S, Vaez A, Khastar H, et al. Electrospun cellulose acetate/gelatin nanofibrous wound dressing containing berberine for diabetic foot ulcer healing: in vitro and in vivo studies. Sci Rep. 2020;10(1). https://doi.org/10.1038/s41598-020-65268-7.

30.

Zeyohanness SS, Abd Hamid H, Zulkifli FH. Poly (vinyl alcohol) electrospun nanofibers containing antimicrobial Rhodomyrtus tomentosa extract. J Bioact Compat Polym. 2018;33(6):585–96. https://doi.org/10.1177/0883911518801040.CrossRef

31.

Jin SG, Yousaf AM, Kim KS, Kim DW, Kim DS, Kim JK, et al. Influence of hydrophilic polymers on functional properties and wound healing efficacy of hydrocolloid based wound dressings. Int J Pharm. 2016;501(1–2):160–6. https://doi.org/10.1016/j.ijpharm.2016.01.044.CrossRefPubMed

32.

Samadian H, Ehterami A, Sarrafzadeh A, Khastar H, Nikbakht M, Rezaei A, et al. Sophisticated polycaprolactone/gelatin nanofibrous nerve guided conduit containing platelet-rich plasma and citicoline for peripheral nerve regeneration: in vitro and in vivo study. Int J Biol Macromol. 2020;150:380–8. https://doi.org/10.1016/j.ijbiomac.2020.02.102.CrossRefPubMed

33.

Barnes CP, Sell SA, Boland ED, Simpson DG, Bowlin GL. Nanofiber technology: designing the next generation of tissue engineering scaffolds. Adv Drug Deliv Rev. 2007;59(14):1413–33. https://doi.org/10.1016/j.addr.2007.04.022.CrossRefPubMed

34.

Nseir N, Regev O, Kaully T, Blumenthal J, Levenberg S, Zussman E. Biodegradable scaffold fabricated of electrospun albumin fibers: mechanical and biological characterization. Tissue Engineering Part C Methods. 2013;19(4):257–64. https://doi.org/10.1089/ten.tec.2012.0118.CrossRefPubMed

35.

Chong EJ, Phan TT, Lim IJ, Zhang Y, Bay BH, Ramakrishna S, et al. Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomater. 2007;3(3):321–30. https://doi.org/10.1016/j.actbio.2007.01.002.CrossRefPubMed

36.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, et al. Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement. Sci Rep. 2016;6(1):24596. https://doi.org/10.1038/srep24596.CrossRefPubMedPubMedCentral

37.

Babu M. Collagen based dressings—a review. Burns. 2000;26(1):54–62.CrossRef

38.

Ono T, Tsuji T, Sakai M, Yukizaki C, Ino H, Akagi I, et al. Induction of hepatocyte growth factor production in human dermal fibroblasts and their proliferation by the extract of bitter melon pulp. Cytokine. 2009;46(1):119–26. https://doi.org/10.1016/j.cyto.2008.12.016.CrossRefPubMed

39.

Kumar R, Balaji S, Sripriya R, Nithya N, Uma T, Sehgal P. In vitro evaluation of antioxidants of fruit extract of Momordica charantia L. on fibroblasts and keratinocytes. J Agric Food Chem. 2010;58(3):1518–22. https://doi.org/10.1021/jf9025986.CrossRefPubMed

Title: Co-electrospun nanofibrous mats loaded with bitter gourd (Momordica charantia) extract as the wound dressing materials: in vitro and in vivo study
Authors: Mohammad Saeid Salami
Gholamreza Bahrami
Elham Arkan
Zhila Izadi
Shahram Miraghaee
Hadi Samadian
Publication date: 01-12-2021
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2021
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-021-03284-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Co-electrospun nanofibrous mats loaded with bitter gourd (Momordica charantia) extract as the wound dressing materials: in vitro and in vivo study

Abstract

Background

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 1/2021

Exploration of the profile-effect relationship of Siraitia grosvenorii aqueous extracts related to their laxative effect on the basis of gray correlation analysis

Characteristics of global naturopathic education, regulation, and practice frameworks: results from an international survey

Echinacea purpurea (L.) Moench treatment of monocytes promotes tonic interferon signaling, increased innate immunity gene expression and DNA repeat hypermethylated silencing of endogenous retroviral sequences

Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart

Antitumor effect and molecular mechanism of fucoidan in NSCLC

Taohuajing reduces oxidative stress and inflammation in diabetic cardiomyopathy through the sirtuin 1/nucleotide-binding oligomerization domain-like receptor protein 3 pathway