Published in:
Open Access
01-12-2022 | Chronic Obstructive Lung Disease | Research
Decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease
Authors:
Antonino Di Stefano, Francesca Dossena, Isabella Gnemmi, Silvestro Ennio D’Anna, Paola Brun, Bruno Balbi, Alessio Piraino, Antonio Spanevello, Francesco Nucera, Vitina Carriero, Francesca Bertolini, Mauro Maniscalco, Ian M. Adcock, Gaetano Caramori, Fabio L. M. Ricciardolo
Published in:
Respiratory Research
|
Issue 1/2022
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Abstract
Background
Identification of COPD patients with a rapid decline in FEV1 is of particular interest for prognostic and therapeutic reasons.
Objective
To determine the expression of markers of inflammation in COPD patients with rapid functional decline in comparison to slow or no decliners.
Methods
In COPD patients monitored for at least 3 years (mean ± SD: 5.8 ± 3 years) for lung functional decline, the expression and localization of inflammatory markers was measured in bronchial biopsies of patients with no lung functional decline (FEV1% + 30 ± 43 ml/year, n = 21), slow (FEV1% ml/year, − 40 ± 19, n = 14) and rapid decline (FEV1% ml/year, − 112 ± 53, n = 15) using immunohistochemistry. ELISA test was used for polymeric immunoglobulin receptor (pIgR) quantitation “in vitro”.
Results
The expression of secretory IgA was significantly reduced in bronchial epithelium (p = 0.011) and plasma cell numbers was significantly reduced in the bronchial lamina propria (p = 0.017) of rapid decliners compared to no decliners. Bronchial inflammatory cell infiltration, CD4, CD8, CD68, CD20, NK, neutrophils, eosinophils, mast cells, pIgR, was not changed in epithelium and lamina propria of rapid decliners compared to other groups. Plasma cells/mm2 correlated positively with scored total IgA in lamina propria of all patients. “In vitro” stimulation of 16HBE cells with LPS (10 μg/ml) and IL-8 (10 ng/ml) induced a significant increase while H2O2 (100 μM) significantly decreased pIgR epithelial expression.
Conclusion
These data show an impaired humoral immune response in rapid decliners with COPD, marked by reduced epithelial secretory IgA and plasma cell numbers in the bronchial lamina propria. These findings may help in the prognostic stratification and treatment of COPD.