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Medical Oncology
Published in: Medical Oncology 1/2012

01-03-2012 | Original paper

Chemotherapy with PLGA microspheres containing docetaxel decreases angiogenesis in human hepatoma xenograft

Authors: Zhi-kui Chen, Min-xian Cai, Jing Yang, Li-wu Lin, En-sheng Xue, Jing Huang, Hong-fen Wei, Xiu-juan Zhang, Li-ming Ke

Published in: Medical Oncology | Issue 1/2012

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Abstract

To investigate the antiangiogenic effect of sustained-release poly (lactic-co-glycolic acid) microspheres containing docetaxel (PMCD) in human hepatoma xenograft. PMCD were prepared by solvent evaporation method with an encapsulation efficiency of 98.7% and a release period of about 3 weeks in vitro. PMCD were intratumorally injected once for mice bearing a human hepatocellular carcinoma. On day 21 post-treatment, the inhibition rate of tumor growth was 72.7% in the high-dose group, indicating a significant antitumor activity. Meanwhile, excellent antiangiogenic effect was observed based on the contrast-enhanced ultrasonography as well as microvessel density determination. Additionally, the real-time fluorescence quantitative PCR revealed that the expressions of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and angiopoietin-2 (Ang2) genes were down-regulated significantly. Interstitial chemotherapy using PMCD was highly effective and safe for the treatment of the human hepatoma xenograft and that decreasing angiogenesis could be one of the most important mechanisms involved in the antitumor activity.
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Metadata
Title
Chemotherapy with PLGA microspheres containing docetaxel decreases angiogenesis in human hepatoma xenograft
Authors
Zhi-kui Chen
Min-xian Cai
Jing Yang
Li-wu Lin
En-sheng Xue
Jing Huang
Hong-fen Wei
Xiu-juan Zhang
Li-ming Ke
Publication date
01-03-2012
Publisher
Springer US
Published in
Medical Oncology / Issue 1/2012
Print ISSN: 1357-0560
Electronic ISSN: 1559-131X
DOI
https://doi.org/10.1007/s12032-010-9762-2

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