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Open Access 01-05-2018 | Glycomics

Chemical glycomics enrichment: imaging the recycling of sialic acid in living cells

Authors: Pierre André Gilormini, Cédric Lion, Dorothée Vicogne, Yann Guérardel, François Foulquier, Christophe Biot

Published in: Journal of Inherited Metabolic Disease | Issue 3/2018

Abstract

The development of metabolic oligosaccharide engineering (MOE) over the past two decades enabled the bioimaging studies of glycosylation processes in physio-pathological contexts. Herein, we successfully applied the chemical reporter strategy to image the fate of sialylated glycoconjugates in healthy and sialin-deficient patient fibroblasts. This chemical glycomics enrichment is a powerful tool for tracking sialylated glycoconjugates and probing lysosomal recycling capacities. Thus, such strategies appear fundamental for the characterization of lysosomal storage diseases.

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Title: Chemical glycomics enrichment: imaging the recycling of sialic acid in living cells
Authors: Pierre André Gilormini
Cédric Lion
Dorothée Vicogne
Yann Guérardel
François Foulquier
Christophe Biot
Publication date: 01-05-2018
Publisher: Springer Netherlands
Published in: Journal of Inherited Metabolic Disease / Issue 3/2018
Print ISSN: 0141-8955
Electronic ISSN: 1573-2665
DOI: https://doi.org/10.1007/s10545-017-0118-3

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