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01-02-2016 | Original Article

Characterization of Rebound Depolarization in Neurons of the Rat Medial Geniculate Body In Vitro

Authors: Xin-Xing Wang, Yan Jin, Hui Sun, Chunlei Ma, Jinsheng Zhang, Ming Wang, Lin Chen

Published in: Neuroscience Bulletin | Issue 1/2016

Abstract

Rebound depolarization (RD) is a response to the offset from hyperpolarization of the neuronal membrane potential and is an important mechanism for the synaptic processing of inhibitory signals. In the present study, we characterized RD in neurons of the rat medial geniculate body (MGB), a nucleus of the auditory thalamus, using whole-cell patch-clamp and brain slices. RD was proportional in strength to the duration and magnitude of the hyperpolarization; was effectively blocked by Ni²⁺ or Mibefradil; and was depressed when the resting membrane potential was hyperpolarized by blocking hyperpolarization-activated cyclic nucleotide-gated (HCN) channels with ZD7288 or by activating G-protein-gated inwardly-rectifying K⁺ (GIRK) channels with baclofen. Our results demonstrated that RD in MGB neurons, which is carried by T-type Ca²⁺ channels, is critically regulated by HCN channels and likely by GIRK channels.

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Title: Characterization of Rebound Depolarization in Neurons of the Rat Medial Geniculate Body In Vitro
Authors: Xin-Xing Wang
Yan Jin
Hui Sun
Chunlei Ma
Jinsheng Zhang
Ming Wang
Lin Chen
Publication date: 01-02-2016
Publisher: Springer Singapore
Published in: Neuroscience Bulletin / Issue 1/2016
Print ISSN: 1673-7067
Electronic ISSN: 1995-8218
DOI: https://doi.org/10.1007/s12264-015-0006-5

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Characterization of Rebound Depolarization in Neurons of the Rat Medial Geniculate Body In Vitro

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