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01-01-2014 | Original Article

Characteristics of alveolar bone associated with physiological movement of molar in mice: a histological and histochemical study

Authors: Kie Matsuda, Maiko Haga-Tsujimura, Sumio Yoshie, Junko Shimomura-Kuroki

Published in: Odontology | Issue 1/2014

Abstract

Mouse molars undergo distal movement, during which new bone is formed at the mesial side of the tooth root whereas the preexisting bone is resorbed at the distal side of the root. However, there is little detailed information available regarding which of the bones that surround the tooth root are involved in physiological tooth movement. In the present study, we therefore aimed to investigate the precise morphological differences of the alveolar bone between the bone formation side of the tooth root, using routine histological procedures including silver impregnation, as well as by immunohistochemical analysis of alkaline phosphatase and tartrate-resistant acid phosphatase activity, and immunohistochemical analysis of the expression of the osteocyte markers dentin matrix protein 1, sclerostin, and fibroblast growth factor 23. Histochemical analysis indicated that bone formation by osteoblasts and bone resorption by osteoclasts occurred at the bone formation side and the bone resorption side, respectively. Osteocyte marker immunoreactivity of osteocytes at the surface of the bone close to the periodontal ligament differed at the bone formation and bone resorption sides. We also showed different specific features of osteocytic lacunar canalicular systems at the bone formation and bone resorption sides by using silver staining. This study suggests that the alveolar bone is different in the osteocyte nature between the bone formation side and the bone resorption side due to physiological distal movement of the mouse molar.

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Title: Characteristics of alveolar bone associated with physiological movement of molar in mice: a histological and histochemical study
Authors: Kie Matsuda
Maiko Haga-Tsujimura
Sumio Yoshie
Junko Shimomura-Kuroki
Publication date: 01-01-2014
Publisher: Springer Japan
Published in: Odontology / Issue 1/2014
Print ISSN: 1618-1247
Electronic ISSN: 1618-1255
DOI: https://doi.org/10.1007/s10266-012-0093-y

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Characteristics of alveolar bone associated with physiological movement of molar in mice: a histological and histochemical study

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