Fig. 2
Detection for physical status of HPV16 infection. a Characterization of HPV-16 integration. Gel photograph of PCR performed on DNA extracted from different tissues, demonstrating failure of amplification of those different fragments (amplimer a, b, c, shown as 475-bp, 477-bp, 276-bp products, respectively) of the E2 gene in CIN and CC lesions. M marker (100-bp DNA ladder), 1 Caski cell, 2 SiHa cell, 3 a CIN I lesion, 4 a CIN III lesion, 5–6 CC lesions (integration), 7 water blank negative control. b Discrimination between episomal infection and mixed infection. Gel photograph of multiplex PCR performed on DNA extracted from paraffin-embedded cervical lesion tissue samples with primers located within the E7 region (315-bp product) and E2 region (amplimers B, 477-bp product) of the HPV-16 genome. M marker, 1 integrated infection (CC), 2 episomal infection (normal cervix), 3 episomal infection (CIN III), 4 mixed infection (CC)