Published in:
Open Access 01-12-2020 | Cervical Cancer | Correction
Correction to: The m6A eraser FTO facilitates proliferation and migration of human cervical cancer cells
Published in: Cancer Cell International | Issue 1/2020
Login to get accessExcerpt
Following publication of the original article [1] the authors have notified us of a few errors in Figures 2, 3 and 6. The corrected Figs. 2, 3, and 6 are presented below.
Fig. 2
FTO regulates cervical cancer cells’ proliferation and migration. a Immunoblot analysis of FTO expression in control and knocking down Hela cells using two different shRNAs; b m6A dot blot assays of Hela cells with or without knocking down of FTO. MB, methylene blue staining (as loading control); c effects of knocking down FTO on Hela cells growth/proliferation. ***P < 0.001. (Student’s t test); d Western blot analysis of FTO expression in control and knocking down SiHa cells using same shRNAs as described in a; e m6A dot blot assays of SiHa cells with or without knocking down of FTO. MB, methylene blue staining (as loading control); f effects of knocking down FTO on SiHa cells growth/proliferation. ***P < 0.001. (Student’s t test); g, h analysis of cell migration capacity using competent or deficient Hela and SiHa cells
Fig. 3
The m6A demethylase activity is required for FTO to play its oncogenic function. a Enforced FTO or FTO-mut expression in Hela cells. FTO-mut carries two point- mutations, H231A and D233A, which disrupt the enzymatic activity of FTO. GAPDH was used as a loading control; b m6A dot blot assays of Hela cells with enforced FTO or FTO-mut expression. MB, methylene blue staining (as loading control); c effects of FTO or mutant FTO overexpression on Hela cells’ growth/proliferation, ***P < 0.001. (Student’s t test); d enforced FTO or FTO-mut expression in SiHa cells. GAPDH was used as a loading control; e m6A dot blot assays of SiHa cells with enforced FTO or FTO-mut expression. MB, methylene blue staining (as loading control); f effects of FTO or mutant FTO overexpression on SiHa cells’ growth/proliferation, ***P < 0.001. (Student’s t test); g, h analysis of cell migration capacity in FTO or mutant FTO overexpressed Hela and SiHa cells
Fig. 6
Overexpression of E2F1 or Myc compensates FTO knocking down effect. a The protein level determination of overexpressed E2F1 or Myc in FTO control and knockdown Hela and SiHa cells, **P < 0.01 (unpaired two-sided t test); b cell proliferation analysis of Hela (upper panel) and SiHa cells (lower panel) in different genetic background as described (a). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t test). c, d The migration analysis of Hela cells (c) and SiHa cells (d) in different genetic background as described in a
× ![]()
× ![]()
× ![]()
…