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Open Access 01-12-2023 | Central Nervous System Trauma | Research

Modification of the height of a weight drop traumatic brain injury model that causes the formation of glial scar and cognitive impairment in rats

Authors: Donny Wisnu Wardhana, Hendy Setyo Yudhanto, Wibi Riawan, Husnul Khotimah, Happy Kurnia Permatasari, Tommy Alfandy Nazwar, Nurdiana Nurdiana

Published in: BMC Neurology | Issue 1/2023

Abstract

Objective

Traumatic brain injury (TBI) is a chronic, progressive condition associated with permanent disabilities, particularly cognitive impairments. Glial scar formation following TBI is considered a contributing factor to these persistent disabilities. Currently, limited research exists on pharmacological interventions targeting glial scar prevention that require a standard weight drop TBI model for glial scar formation. Since there is no established standard TBI model for glial scar formation, this study aims to validate and modify the height of the weight drop model to identify glial scar formation and cognitive impairments.

Methods

Fifteen male Sprague Dawley rats were randomly divided into sham, WD1, and WD2 groups. The weight drop model with a 10 g load was applied to the right exposed brain of the rats from a height of 5 cm (WD1) and 10 cm (WD2) using a modified Feeney’s weight drop device. Cognitive impairments were confirmed using the novel object recognition (NOR) test with ethovision software on day 15. Subsequently, the rats were decapitated on day 16, and GFAP immunohistochemical staining was performed to confirm the presence of glial scarring.

Results

The WD1 and WD2 groups exhibited a significant increase in glial scar formation compared to the sham group, with the WD2 group resulting in even more pronounced glial scar formation. Only the WD2 model caused statistically significant cognitive damage. The negative correlation coefficient indicates that an increase in GFAP + cells will decrease the cognitive function.

Conclusion

Modification of the height of the weight drop model, by dropping a weight of 10 g from a height of 10 cm (WD2 group) onto the right brain exposed of the rat has been proven to induce the formation of a glial scar and cognitive impairment.

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Title: Modification of the height of a weight drop traumatic brain injury model that causes the formation of glial scar and cognitive impairment in rats
Authors: Donny Wisnu Wardhana
Hendy Setyo Yudhanto
Wibi Riawan
Husnul Khotimah
Happy Kurnia Permatasari
Tommy Alfandy Nazwar
Nurdiana Nurdiana
Publication date: 01-12-2023
Publisher: BioMed Central
Keyword: Central Nervous System Trauma
Published in: BMC Neurology / Issue 1/2023
Electronic ISSN: 1471-2377
DOI: https://doi.org/10.1186/s12883-023-03494-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Modification of the height of a weight drop traumatic brain injury model that causes the formation of glial scar and cognitive impairment in rats

Abstract

Objective

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Methods

Results

Conclusion

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