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Maxillofacial Plastic and Reconstructive Surgery

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Open Access 01-12-2016 | Research

Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration

Authors: Chae-Kyung Yoo, Jae-Yun Jeon, You-Jin Kim, Seong-Gon Kim, Kyung-Gyun Hwang

Published in: Maxillofacial Plastic and Reconstructive Surgery | Issue 1/2016

Abstract

Background

The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR).

Methods

Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining.

Results

Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly different between the two groups (P > 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (P < 0.05).

Conclusions

These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.

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Title: Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration
Authors: Chae-Kyung Yoo
Jae-Yun Jeon
You-Jin Kim
Seong-Gon Kim
Kyung-Gyun Hwang
Publication date: 01-12-2016
Publisher: Springer Berlin Heidelberg
Published in: Maxillofacial Plastic and Reconstructive Surgery / Issue 1/2016
Electronic ISSN: 2288-8586
DOI: https://doi.org/10.1186/s40902-016-0062-4

At a glance: The STEP trials

Springer Medicine

Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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