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Allergy, Asthma & Clinical Immunology
Published in: Allergy, Asthma & Clinical Immunology 1/2022

Open Access 01-12-2022 | Bronchial Asthma | Research

KIF2A decreases IL-33 production and attenuates allergic asthmatic inflammation

Authors: Zhengxia Wang, Jingjing Wu, Jingxian Jiang, Qiyun Ma, Meijuan Song, Tingting Xu, Yanan Liu, Zhongqi Chen, Yanmin Bao, Mao Huang, Mingshun Zhang, Ningfei Ji

Published in: Allergy, Asthma & Clinical Immunology | Issue 1/2022

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Abstract

Background

The microtubule-dependent molecular motor protein Kinesin Family Member 2A (KIF2A) is down-regulated in asthmatic human airway epithelium. However, little is known about the roles of KIF2A as well as the possible underlying mechanisms in asthma.

Methods

House dust mite (HDM) extract was administered to establish a murine model of asthma. The expression of KIF2A, IL-33 and the autophagy pathways were detected. The plasmid pCMV-KIF2A was used to overexpress KIF2A in the airway epithelial cells in vitro and in vivo. IL-4, IL-5, IL-33 and other cytokines in bronchoalveolar lavage fluid (BALF) and lung tissues homogenates were measured.

Results

In response to the challenge of house dust mite (HDM) in vitro and in vivo, airway epithelial cells displayed decreased production of KIF2A. Meanwhile, autophagy and IL-33 were increased in HMD-treated epithelial cells. Mechanistically, KIF2A decreased autophagy via suppressing mTORC1 pathway in HDM-treated epithelial cells, which contributed to the reduced production of IL-33. Moreover, in vivo KIF2A transfection reduced IL-33 and autophagy in the lung, leading to the attenuation of allergic asthma.

Conclusion

KIF2A suppressed mTORC1-mediated autophagy and decreased the production of epithelial-derived cytokine IL-33 in allergic airway inflammation. These data indicate that KIF2A may be a novel target in allergic asthma.
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Metadata
Title
KIF2A decreases IL-33 production and attenuates allergic asthmatic inflammation
Authors
Zhengxia Wang
Jingjing Wu
Jingxian Jiang
Qiyun Ma
Meijuan Song
Tingting Xu
Yanan Liu
Zhongqi Chen
Yanmin Bao
Mao Huang
Mingshun Zhang
Ningfei Ji
Publication date
01-12-2022
Publisher
BioMed Central
Published in
Allergy, Asthma & Clinical Immunology / Issue 1/2022
Electronic ISSN: 1710-1492
DOI
https://doi.org/10.1186/s13223-022-00697-9

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