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Inflammopharmacology

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01-10-2019 | Breast Cancer | Original Article

Naringenin inhibits migration of breast cancer cells via inflammatory and apoptosis cell signaling pathways

Authors: Zhenjiang Zhao, Guoguo Jin, Yinghui Ge, Zhiping Guo

Published in: Inflammopharmacology | Issue 5/2019

Abstract

Background

Naringenin, a flavonoid compound, has a wide variety of uses in the pharmaceutical industry for its antioxidant and anti-inflammatory potential.

Objectives

The current experiment aimed to investigate the anticancer effect of naringenin in triple-negative human breast cancer cells (MDA-MR-231) and an animal model with 7,12-dimethylbenz[a] anthracene (DMBA)-induced breast cancer in female rats to determine the mechanisms and molecular targets.

Methods

The cytotoxic effects of naringenin against MDA-MB-231 cells were assessed by MTT assay. Apoptosis and cell cycle alterations were analyzed via flow cytometry. Morphological and biochemical changes in DMBA-induced cancer with naringenin treatment were assayed using our protocol. The potential mechanisms of action were verified via qRT-PCR.

Results

Naringenin was found to inhibit cell proliferation in a time- and concentration-dependent manner. This effect was associated with cell cycle arrest at the G0/G1 phase, along with apoptosis and deposition at the sub-G1 phase (75%). Treatment with naringenin reduced tumor incidence (45.55, 40, and 27.67%) and tumor burden (78.7, 35.4, and 1.2 g) in a dose-dependent manner. Naringenin treatment altered the biochemical and antioxidant parameters related to inflammation necessary for anticancer activity. The qRT-PCR studies further confirmed the mitochondrial-mediated apoptotic effects of naringenin.

Conclusion

On the basis of these results, we can conclude that naringenin exerts an anticancer effect in the MDA-MB-231 cell line that arrests cell development at the G0/G1 phase, and in vivo it alters the mitochondrial-mediated intrinsic pathway responsible for apoptosis.

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Title: Naringenin inhibits migration of breast cancer cells via inflammatory and apoptosis cell signaling pathways
Authors: Zhenjiang Zhao
Guoguo Jin
Yinghui Ge
Zhiping Guo
Publication date: 01-10-2019
Publisher: Springer International Publishing
Keywords: Breast Cancer
Breast Cancer
Published in: Inflammopharmacology / Issue 5/2019
Print ISSN: 0925-4692
Electronic ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-018-00556-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Naringenin inhibits migration of breast cancer cells via inflammatory and apoptosis cell signaling pathways

Abstract

Background

Objectives

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Objectives

Methods

Results

Conclusion

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