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BMC Oral Health
Published in: BMC Oral Health 1/2023

Open Access 01-12-2023 | Bleeding on Probing | Research

Diagnostic potential of miR-200 family members in gingival crevicular fluid for chronic periodontitis: correlation with clinical parameters and therapeutic implications

Author: Shi-Lei Yu

Published in: BMC Oral Health | Issue 1/2023

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Abstract

Objective

The purpose of this study was to evaluate the potential of miR-200 family members in gingival crevicular fluid (GCF) as diagnostic biomarkers for chronic periodontitis (CP), aiming to provide valuable insights for the early detection and management of the disease.

Methods

GSE89081 dataset profiled miRNAs in GCF derived from 5 healthy and 5 periodontitis was analyzed by GEO2R. Quantitative real-time PCR was used to quantify the expression levels of miR-200 family members (miR-200a-3p, miR-200a-5p, miR-200b-3p, miR-200b-5p, miR-200c-3p, miR-200c-5p, miR-141-3p, miR-141-5p, and miR-429) in the GCF samples from 103 CP patients and 113 healthy controls. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic potential of miR-200 family members in differentiating CP patients from healthy controls.

Results

By analyzing the GSE89081 dataset, miR-200a-5p, miR-200b-5p and miR-200c-5p were significantly upregulated in GCF of the CP patients compared to the healthy control. In this study, miR-200a-3p, miR-200a-5p, miR-200b-3p, miR-200b-5p, miR-200c-3p, miR-200c-5p were significantly increased in GCF of CP patients compared to the healthy control, while miR-141 and miR-429 did not show significant differences. MiR-200a, -200b and 200c had good diagnostic value, and when these miRNAs were combined, they demonstrated excellent diagnostic value for CP with an AUC of 0.997, sensitivity of 99.03%, and specificity of 98.23%. MiR-200a, -200b and 200c in GCF showed significant and positive correlation with plaque index (PI), gingival index (GI), bleeding on probing (BOP), clinical attachment level (CAL), and probing pocket depth (PPD).

Conclusion

MiR-200a, -200b and 200c in GCF may serve as potential biomarkers for the early diagnosis of CP, which was correlated with clinical parameters, being therapeutic targets for CP.
Literature
1.
Cardoso EM, Reis C, Manzanares-Cespedes MC. Chronic periodontitis, inflammatory cytokines, and interrelationship with other chronic diseases. Postgrad Med. 2018;130(1):98–104.CrossRefPubMed
2.
Ji S, Choi YS, Choi Y. Bacterial invasion and persistence: critical events in the pathogenesis of periodontitis? J Periodontal Res. 2015;50(5):570–85.CrossRefPubMed
3.
Vidal-Gutierrez X, Prado-Prone G, Rodil SE, Velasquillo C, Clemente I, Silva-Bermudez P, Almaguer-Flores A. Bismuth subsalicylate incorporated in polycaprolactone-gelatin membranes by electrospinning to prevent bacterial colonization. Biomed Mater 2021, 16(4).
4.
Zhang Q, Li Z, Wang C, Shen T, Yang Y, Chotivichien S, Wang L. Prevalence and predictors for periodontitis among adults in China, 2010. Glob Health Action. 2014;7:24503.CrossRefPubMed
5.
Kapila YL. Oral health’s inextricable connection to systemic health: special populations bring to bear multimodal relationships and factors connecting periodontal disease to systemic diseases and conditions. Periodontol 2000. 2021;87(1):11–6.CrossRefPubMedPubMedCentral
6.
Nazir MA. Prevalence of periodontal disease, its association with systemic diseases and prevention. Int J Health Sci (Qassim). 2017;11(2):72–80.PubMed
7.
Heidari Z, Moudi B, Mahmoudzadeh-Sagheb H. Immunomodulatory factors gene polymorphisms in chronic periodontitis: an overview. BMC Oral Health. 2019;19(1):29.CrossRefPubMedPubMedCentral
8.
Barros SP, Williams R, Offenbacher S, Morelli T. Gingival crevicular fluid as a source of biomarkers for periodontitis. Periodontol 2000. 2016;70(1):53–64.CrossRefPubMedPubMedCentral
9.
Ho PTB, Clark IM, Le LTT. MicroRNA-Based diagnosis and therapy. Int J Mol Sci 2022, 23(13).
10.
Saito A, Horie M, Ejiri K, Aoki A, Katagiri S, Maekawa S, Suzuki S, Kong S, Yamauchi T, Yamaguchi Y, et al. MicroRNA profiling in gingival crevicular fluid of periodontitis-a pilot study. FEBS Open Bio. 2017;7(7):981–94.CrossRefPubMedPubMedCentral
11.
Rovas A, Puriene A, Snipaitiene K, Punceviciene E, Buragaite-Staponkiene B, Matuleviciute R, Butrimiene I, Jarmalaite S. Analysis of periodontitis-associated miRNAs in gingival tissue, gingival crevicular fluid, saliva and blood plasma. Arch Oral Biol. 2021;126:105125.CrossRefPubMed
12.
Mico-Martinez P, Garcia-Gimenez JL, Seco-Cervera M, Lopez-Roldan A, Alminana-Pastor PJ, Alpiste-Illueca F, Pallardo FV. miR-1226 detection in GCF as potential biomarker of chronic periodontitis: a pilot study. Med Oral Patol Oral Cir Bucal. 2018;23(3):e308–14.PubMedPubMedCentral
13.
Lewis A, Felice C, Kumagai T, Lai C, Singh K, Jeffery RR, Feakins R, Giannoulatou E, Armuzzi A, Jawad N, et al. The miR-200 family is increased in dysplastic lesions in ulcerative colitis patients. PLoS ONE. 2017;12(3):e0173664.CrossRefPubMedPubMedCentral
14.
Zidar N, Bostjancic E, Jerala M, Kojc N, Drobne D, Stabuc B, Glavac D. Down-regulation of microRNAs of the miR-200 family and up-regulation of snail and slug in inflammatory bowel diseases - hallmark of epithelial-mesenchymal transition. J Cell Mol Med. 2016;20(10):1813–20.CrossRefPubMedPubMedCentral
15.
Chen X, Ling Y, Wei Y, Tang J, Ren Y, Zhang B, Jiang F, Li H, Wang R, Wen W, et al. Dual regulation of HMGB1 by combined JNK1/2-ATF2 axis with miR-200 family in nonalcoholic steatohepatitis in mice. FASEB J. 2018;32(5):2722–34.CrossRefPubMed
16.
Polizzi A, Quinzi V, Ronsivalle V, Venezia P, Santonocito S, Lo Giudice A, Leonardi R, Isola G. Tooth automatic segmentation from CBCT images: a systematic review. Clin Oral Investig 2023.
17.
Dhawan A, Buffa FM. Machine learning using gene-sets to infer miRNA function. Adv Exp Med Biol. 2022;1385:229–40.CrossRefPubMed
18.
Wirth H, Cakir MV, Hopp L, Binder H. Analysis of microRNA expression using machine learning. Methods Mol Biol. 2014;1107:257–78.CrossRefPubMed
19.
Isola G, Santonocito S, Distefano A, Polizzi A, Vaccaro M, Raciti G, Alibrandi A, Li Volti G. Impact of periodontitis on gingival crevicular fluid miRNAs profiles associated with cardiovascular disease risk. J Periodontal Res. 2023;58(1):165–74.CrossRefPubMed
20.
Isola G, Giudice AL, Polizzi A, Alibrandi A, Patini R, Ferlito S. Periodontitis and tooth loss have negative systemic impact on circulating progenitor cell levels: a clinical study. Genes (Basel) 2019, 10(12).
21.
Kornman KS, Papapanou PN. Clinical application of the new classification of periodontal diseases: ground rules, clarifications and “gray zones. J Periodontol. 2020;91(3):352–60.CrossRefPubMed
22.
Isola G, Polizzi A, Muraglie S, Leonardi R, Lo Giudice A. Assessment of Vitamin C and Antioxidant Profiles in Saliva and Serum in Patients with Periodontitis and Ischemic Heart Disease. Nutrients 2019, 11(12).
23.
Isola G, Polizzi A, Ronsivalle V, Alibrandi A, Palazzo G, Lo Giudice A. Impact of Matrix Metalloproteinase-9 during Periodontitis and Cardiovascular Diseases. Molecules 2021, 26(6).
24.
Fatima T, Khurshid Z, Rehman A, Imran E, Srivastava KC, Shrivastava D. Gingival Crevicular Fluid (GCF): a Diagnostic Tool for the detection of Periodontal Health and Diseases. Molecules 2021, 26(5).
25.
Zhu J, Zhong Z. The expression and clinical significance of miR-30b-3p and miR-125b-1-3p in patients with periodontitis. BMC Oral Health. 2022;22(1):325.CrossRefPubMedPubMedCentral
26.
Han P, Bartold PM, Ivanovski S. The emerging role of small extracellular vesicles in saliva and gingival crevicular fluid as diagnostics for periodontitis. J Periodontal Res. 2022;57(1):219–31.CrossRefPubMed
27.
Fujimori K, Yoneda T, Tomofuji T, Ekuni D, Azuma T, Maruyama T, Mizuno H, Sugiura Y, Morita M. Detection of salivary miRNAs reflecting chronic periodontitis: a pilot study. Molecules 2019, 24(6).
28.
Klages U, Rost F, Wehrbein H, Zentner A. Perception of occlusion, psychological impact of dental esthetics, history of orthodontic treatment and their relation to oral health in naval recruits. Angle Orthod. 2007;77(4):675–80.CrossRefPubMed
29.
Elazazy O, Amr K, Abd El Fattah A, Abouzaid M. Evaluation of serum and gingival crevicular fluid microRNA-223, microRNA-203 and microRNA-200b expression in chronic periodontitis patients with and without diabetes type 2. Arch Oral Biol. 2021;121:104949.CrossRefPubMed
30.
Santonocito S, Polizzi A, Palazzo G, Isola G. The emerging role of microRNA in Periodontitis: pathophysiology, clinical potential and future molecular perspectives. Int J Mol Sci 2021, 22(11).
31.
Hsieh PL, Huang CC, Yu CC. Emerging role of MicroRNA-200 family in Dentistry. Noncoding RNA 2021, 7(2).
32.
Sweat M, Sweat Y, Yu W, Su D, Leonard RJ, Eliason SL, Amendt BA. The miR-200 family is required for ectodermal organ development through the regulation of the epithelial stem cell niche. Stem Cells. 2021;39(6):761–75.CrossRefPubMed
33.
Amaral SA, Pereira TSF, Brito JAR, Cortelli SC, Cortelli JR, Gomez RS, Costa FO, Miranda Cota LO. Comparison of miRNA expression profiles in individuals with chronic or aggressive periodontitis. Oral Dis. 2019;25(2):561–8.CrossRefPubMed
34.
Feng YY, Xu XQ, Ji CB, Shi CM, Guo XR, Fu JF. Aberrant hepatic microRNA expression in nonalcoholic fatty liver disease. Cell Physiol Biochem. 2014;34(6):1983–97.CrossRefPubMed
35.
Van Keuren-Jensen KR, Malenica I, Courtright AL, Ghaffari LT, Starr AP, Metpally RP, Beecroft TA, Carlson EW, Kiefer JA, Pockros PJ, et al. microRNA changes in liver tissue associated with fibrosis progression in patients with hepatitis C. Liver Int. 2016;36(3):334–43.CrossRefPubMed
36.
Fujimori K, Yoneda T, Tomofuji T, Ekuni D, Azuma T, Maruyama T, Sugiura Y, Morita M. Detection of salivary miRNAs that predict chronic Periodontitis Progression: a Cohort Study. Int J Environ Res Public Health 2021, 18(15).
37.
Ogata Y, Matsui S, Kato A, Zhou L, Nakayama Y, Takai H. MicroRNA expression in inflamed and noninflamed gingival tissues from japanese patients. J Oral Sci. 2014;56(4):253–60.CrossRefPubMed
38.
Kalea AZ, Hoteit R, Suvan J, Lovering RC, Palmen J, Cooper JA, Khodiyar VK, Harrington Z, Humphries SE, D’Aiuto F. Upregulation of gingival tissue miR-200b in obese periodontitis subjects. J Dent Res. 2015;94(3 Suppl):59S–69S.CrossRefPubMedPubMedCentral
39.
Matsui S, Zhou L, Nakayama Y, Mezawa M, Kato A, Suzuki N, Tanabe N, Nakayama T, Suzuki Y, Kamio N, et al. MiR-200b attenuates IL-6 production through IKKbeta and ZEB1 in human gingival fibroblasts. Inflamm Res. 2018;67(11–12):965–73.CrossRefPubMedPubMedCentral
40.
Akkouch A, Zhu M, Romero-Bustillos M, Eliason S, Qian F, Salem AK, Amendt BA, Hong L. MicroRNA-200c attenuates Periodontitis by modulating Proinflammatory and Osteoclastogenic Mediators. Stem Cells Dev. 2019;28(15):1026–36.CrossRefPubMedPubMedCentral
41.
Hong L, Sharp T, Khorsand B, Fischer C, Eliason S, Salem A, Akkouch A, Brogden K, Amendt BA. MicroRNA-200c represses IL-6, IL-8, and CCL-5 expression and enhances osteogenic differentiation. PLoS ONE. 2016;11(8):e0160915.CrossRefPubMedPubMedCentral
42.
Kwon EJ, Kim HJ, Woo BH, Joo JY, Kim YH, Park HR. Profiling of plasma-derived exosomal RNA expression in patients with periodontitis: a pilot study. Oral Dis 2022.
43.
Stoecklin-Wasmer C, Guarnieri P, Celenti R, Demmer RT, Kebschull M, Papapanou PN. MicroRNAs and their target genes in gingival tissues. J Dent Res. 2012;91(10):934–40.CrossRefPubMedPubMedCentral
44.
Wendlandt EB, Graff JW, Gioannini TL, McCaffrey AP, Wilson ME. The role of microRNAs miR-200b and miR-200c in TLR4 signaling and NF-kappaB activation. Innate Immun. 2012;18(6):846–55.CrossRefPubMed
45.
Chukkapalli SS, Ambadapadi S, Varkoly K, Jiron J, Aguirre JI, Bhattacharyya I, Morel LM, Lucas AR, Kesavalu L. Impaired innate immune signaling due to combined toll-like receptor 2 and 4 deficiency affects both periodontitis and atherosclerosis in response to polybacterial infection. Pathog Dis 2018, 76(8).
46.
Huang B, Chen H, Fan M. Inhibition of TLR4 signaling pathway: molecular treatment strategy of periodontitis-associated atherosclerosis. Med Hypotheses. 2008;70(3):614–7.CrossRefPubMed
Metadata
Title
Diagnostic potential of miR-200 family members in gingival crevicular fluid for chronic periodontitis: correlation with clinical parameters and therapeutic implications
Author
Shi-Lei Yu
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2023
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-023-03174-w

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