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Cancer Cell International
Published in: Cancer Cell International 1/2020

Open Access 01-12-2020 | Biomarkers | Primary research

Biomarkers to quantify cell migration characteristics

Authors: Sangwoo Kwon, Woochul Yang, Donggerami Moon, Kyung Sook Kim

Published in: Cancer Cell International | Issue 1/2020

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Abstract

Background

Because cell movement is primarily driven by the connection between F-actin and integrin through a physical linkage, cellular elasticity and adhesion strength have been considered as biomarkers of cell motility. However, a consistent set of biomarkers that indicate the potential for cell motility is still lacking.

Methods

In this work, we characterize a phenotype of cell migration in terms of cellular elasticity and adhesion strength, which reveals the interdependence of subcellular systems that mediate optimal cell migration.

Results

Stiff cells weakly adhered to the substrate revealed superior motility, while soft cell migration with strong adhesion was relatively inhibited. The spatial distribution and amount of F-actin and integrin were highly variable depending on cell type, but their density exhibited linear correlations with cellular elasticity and adhesion strength, respectively.

Conclusions

The densities of F-actin and integrin exhibited linear correlations with cellular elasticity and adhesion strength, respectively, therefore, they can be considered as biomarkers to quantify cell migration characteristics.
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Metadata
Title
Biomarkers to quantify cell migration characteristics
Authors
Sangwoo Kwon
Woochul Yang
Donggerami Moon
Kyung Sook Kim
Publication date
01-12-2020
Publisher
BioMed Central
Keyword
Biomarkers
Published in
Cancer Cell International / Issue 1/2020
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-020-01312-w

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