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Published in:

01-03-2019 | Original Article

Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars

Authors: Tiago Silva da Fonseca, Guilherme F. Silva, Juliane M. Guerreiro-Tanomaru, Mateus Machado Delfino, Estela Sasso-Cerri, Mário Tanomaru-Filho, Paulo Sérgio Cerri

Published in: Clinical Oral Investigations | Issue 3/2019

Abstract

Objectives

Evaluate the tissue reaction of periodontium subjacent to furcation perforations in rat molars sealed with Biodentine or mineral trioxide aggregate (MTA).

Materials and methods

The pulp chamber floor of right upper first molars of 60 rats was perforated and filled with Biodentine, MTA, or cotton pellet (sham); the left first molars were used as control. After 7, 15, 30, and 60 days, maxillary fragments were processed for paraffin-embedding. The periodontal space (PS), volume density of inflammatory cells (VvIC) and fibroblasts (VvFb), number of osteoclasts, and collagen content were obtained. Interleukin-6 (IL-6) and osterix (osteoblast marker) were detected by immunohistochemistry. The data were submitted to ANOVA and Tukey’s test (p ≤ 0.05).

Results

At 7 days, high values in VvIC, IL-6-immunolabeled cells, and osteoclasts were accompanied by reduced collagen content in enlarged PS of experimental groups. At all periods, VvIC, number of osteoclasts and IL-6, and PS were higher in sham than in Biodentine and MTA (p < 0.0001). From 7 to 60 days, significant reduction in VvIC, IL-6 immunoexpression, and osteoclasts was accompanied by significant increase in VvFb, osteoblasts, and collagen in Biodentine and MTA groups. At 60 days, significant differences in VvIC, PS, IL-6, osteoclasts, and osteoblasts were not found between Biodentine and MTA. Significant differences in the osteoclast number were not observed among Biodentine, MTA, and control groups while osteoblasts number was higher in Biodentine and MTA groups.

Conclusions

Despite the initial inflammatory reaction and bone resorption, the sealing of furcation perforations with Biodentine and MTA favors the repair of periodontal tissues.

Clinical relevance

Biodentine and MTA exhibit potential as repair material in the treatment of furcation perforations.

Mente J, Leo M, Panagidis D, Saure D, Pfefferle T (2014) Treatment outcome of mineral trioxide aggregate: repair of root perforations-long-term results. J Endod 40:790–796. https://doi.org/10.1016/j.joen.2014.02.003 CrossRefPubMed

da Silva GF, Guerreiro-Tanomaru JM, Sasso-Cerri E, Tanomaru-Filho M, Cerri PS (2011) Histological and histomorphometrical evaluation of furcation perforations filled with MTA, CPM and ZOE. Int Endod J 44:100–110. https://doi.org/10.1111/j.1365-2591.2010.01803.x CrossRefPubMed

Silva LAB, Pieroni KAMG, Nelson-Filho P, Silva RAB, Hernandéz-Gatón P, Lucisano MP, Paula-Silva FWG, de Queiroz AM (2017) Furcation perforation: periradicular tissue response to biodentine as a repair material by histopathologic and indirect immunofluorescence analyses. J Endod 43:1137–1142. https://doi.org/10.1016/j.joen.2017.02.001 CrossRefPubMed

Lee SJ, Monsef M, Torabinejad M (1993) Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod 19:541–544. https://doi.org/10.1016/S0099-2399(06)81282-3 CrossRefPubMed

Camilleri J, Sorrentino F, Damidot D (2013) Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, Biodentine and MTA Angelus. Dent Mater 29:580–593. https://doi.org/10.1016/j.dental.2013.03.007 CrossRefPubMed

da Fonseca TS, da Silva GF, Tanomaru-Filho M, Sasso-Cerri E, Guerreiro-Tanomaru JM, Cerri PS (2016) In vivo evaluation of the inflammatory response and IL-6 immunoexpression promoted by Biodentine and MTA Angelus. Int Endod J 49:145–153. https://doi.org/10.1111/iej.12435 CrossRefPubMed

Rodrigues EM, Gomes-Cornélio AL, Soares-Costa A, Salles LP, Velayutham M, Rossa-Junior C, Guerreiro-Tanomaru JM, Tanomaru-Filho M (2017) An assessment of the overexpression of BMP-2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine. Int Endod J 50:e9–e18. https://doi.org/10.1111/iej.12745 CrossRefPubMed

Tawil PZ, Duggan DJ, Galicia JC (2015) Mineral trioxide aggregate (MTA): its history, composition, and clinical applications. Compend Contin Educ Dent 36:247–252PubMedPubMedCentral

De Rossi A, Silva LAB, Gatón-Hernández P, Sousa-Neto MD, Nelson-Filho P, Silva RA, de Queiroz AM (2014) Comparison of pulpal responses to pulpotomy and pulp capping with Biodentine and mineral trioxide aggregate in dogs. J Endod 40:1362–1369. https://doi.org/10.1016/j.joen.2014.02.006 CrossRefPubMed

10.

Bosso-Martelo R, Guerreiro-Tanomaru JM, Viapiana R, Berbert FL, Duarte MA, Tanomaru-Filho M (2016) Physicochemical properties of calcium silicate cements associated with microparticulate and nanoparticulate radiopacifiers. Clin Oral Investig 20:83–90. https://doi.org/10.1007/s00784-015-1483-7 CrossRefPubMed

11.

Silva GF, Guerreiro-Tanomaru JM, da Fonseca TS, Bernardi MIB, Sasso-Cerri E, Tanomaru-Filho M, Cerri PS (2017) Zirconium oxide and niobium oxide used as radiopacifiers in a calcium silicate-based material stimulate fibroblast proliferation and collagen formation. Int Endod J 50:e95–e108. https://doi.org/10.1111/iej.12789 CrossRefPubMed

12.

Silva GF, Tanomaru-Filho M, Bernardi MIB, Guerreiro-Tanomaru JM, Cerri PS (2015) Niobium pentoxide as radiopacifying agent of calcium silicate-based material: evaluation of physicochemical and biological properties. Clin Oral Investig 19:2015–2025. https://doi.org/10.1007/s00784-015-1412-9 CrossRefPubMed

13.

Marciano MA, Costa RM, Camilleri J, Mondelli RF, Guimarães BM, Duarte MA (2014) Assessment of color stability of white mineral trioxide aggregate Angelus and bismuth oxide in contact with tooth structure. J Endod 40:1235–1240. https://doi.org/10.1016/j.joen.2014.01.044 CrossRefPubMed

14.

Grazziotin-Soares R, Nekoofar MH, Davies TE, Bafail A, Alhaddar E, Hübler R, Busato AL, Dummer PM (2014) Effect of bismuth oxide on white mineral trioxide aggregate: chemical characterization and physical properties. Int Endod J 47:520–533. https://doi.org/10.1111/iej.12181 CrossRefPubMed

15.

Camilleri J, Montesin FE, Papaioannou S, McDonald F, Pitt Ford TR (2004) Biocompatibility of two commercial forms of mineral trioxide aggregate. Int Endod J 37:699–704. https://doi.org/10.1111/j.1365-2591.2004.00859.x CrossRefPubMed

16.

Rajasekharan S, Martens LC, Cauwels RGEC, Verbeeck RMH (2014) Biodentine^TM material characteristics and clinical applications: a review of the literature. Eur Arch Paediatr Dent 15:147–158. https://doi.org/10.1007/s40368-014-0114-3 CrossRefPubMed

17.

About I (2016) Biodentine: from biochemical and bioactive properties to clinical applications. G Ital Endod 30:81–88. https://doi.org/10.1016/j.gien.2016.09.002 CrossRef

18.

Camilleri J, Kralj P, Veber M, Sinagra E (2012) Characterization and analyses of acid-extractable and leached trace elements in dental cements. Int Endod J 45:737–743. https://doi.org/10.1111/j.1365-2591.2012.02027.x CrossRefPubMed

19.

Cuadros-Fernández C, Lorente Rodríguez AI, Sáez-Martínez S, García-Binimelis J, About I, Mercadé M (2016) Short-term treatment outcome of pulpotomies in primary molars using mineral trioxide aggregate and Biodentine: a randomized clinical trial. Clin Oral Investig 20:1639–1645. https://doi.org/10.1007/s00784-015-1656-4 CrossRefPubMed

20.

Camilleri J (2013) Investigation of Biodentine as dentine replacement material. J Dent 41:600–610. https://doi.org/10.1016/j.jdent.2013.05.003 CrossRefPubMed

21.

Nowicka A, Lipski M, Parafiniuk M, Sporniak-Tutak K, Lichota D, Kosierkiewicz A, Kaczmarek W, Buczkowska-Radlińska J (2013) Response of human dental pulp capped with Biodentine and mineral trioxide aggregate. J Endod 39:743–747. https://doi.org/10.1016/j.joen.2013.01.005 CrossRefPubMed

22.

Tran XV, Gorin C, Willig C, Baroukh B, Pellat B, Decup F, Opsahl Vital S, Chaussain C, Boukpessi T (2012) Effect of a calcium-silicate-based restorative cement on pulp repair. J Dent Res 91:1166–1171. https://doi.org/10.1177/0022034512460833 CrossRefPubMed

23.

Camilleri J, Grech L, Galea K, Keir D, Fenech M, Formosa L, Damidot D, Mallia B (2014) Porosity and root dentine to material interface assessment of calcium silicate-based root-end filling materials. Clin Oral Investig 18:1437–1446. https://doi.org/10.1007/s00784-013-1124-y CrossRefPubMed

24.

Gomes-Cornélio AL, Rodrigues EM, Salles LP, Mestieri LB, Faria G, Guerreiro-Tanomaru JM, Tanomaru-Filho M (2017) Bioactivity of MTA Plus, Biodentine and an experimental calcium silicate-based cement on human osteoblast-like cells. Int Endod J 50:39–47. https://doi.org/10.1111/iej.12589 CrossRefPubMed

25.

Corral Nuñez CM, Bosomworth HJ, Field C, Whitworth JM, Valentine RA (2014) Biodentine and mineral trioxide aggregate induce similar cellular responses in a fibroblast cell line. J Endod 40:406–411. https://doi.org/10.1016/j.joen.2013.11.006 CrossRefPubMed

26.

Hakki SS, Bozkurt SB, Ozcopur B, Purali N, Belli S (2012) Periodontal ligament fibroblast response to root perforations restored with different materials: a laboratory study. Int Endod J 45:240–248. https://doi.org/10.1111/j.1365-2591.2011.01968.x CrossRefPubMed

27.

Zairi A, Lambrianidis T, Pantelidou O, Papadimitriou S, Tziafas D (2012) Periradicular tissue responses to biologically active molecules or MTA when applied in furcal perforation of dog’s teeth. Int J Dent 2012:257832. https://doi.org/10.1155/2012/257832 CrossRefPubMedPubMedCentral

28.

de Oliveira PA, de Pizzol-Júnior JP, Longhini R, Sasso-Cerri E, Cerri PS (2017) Cimetidine reduces interleukin-6, matrix metalloproteinases-1 and -9 immunoexpression in the gingival mucosa of rat molars with induced periodontal disease. J Periodontol 88:100–111. https://doi.org/10.1902/jop.2016.160132 CrossRefPubMed

29.

Cerri PS, Boabaid F, Katchburian E (2003) Combined TUNEL and TRAP methods suggest that apoptotic bone cells are inside vacuoles of alveolar bone osteoclasts in young rats. J Periodontal Res 38:223–226CrossRefPubMed

30.

Castro-Raucci LMS, Teixeira LN, Oliveira IR, Raucci-Neto W, Jacobovitz M, Rosa AL, de Oliveira PT (2017) Osteogenic cell response to calcium aluminate-based cement. Int Endod J 50:771–779. https://doi.org/10.1111/iej.12682 CrossRefPubMed

31.

Baek W-Y, de Crombrugghe B, Kim J-E (2010) Postnatally induced inactivation of osterix in osteoblasts results in the reduction of bone formation and maintenance. Bone 46:920–928. https://doi.org/10.1016/j.bone.2009.12.007 CrossRefPubMed

32.

Manni ML, Czajka CA, Oury TD, Gilbert TW (2011) Extracellular matrix powder protects against bleomycin-induced pulmonary fibrosis. Tissue Eng Part A 17:2795–2804. https://doi.org/10.1089/ten.tea.2011.0023 CrossRefPubMedPubMedCentral

33.

Schroeder H, Listgarten MA (1997) The gingival tissues: the architecture of periodontal protection. Periodontol 2000 13:91–120CrossRefPubMed

34.

Pitt Ford TR, Torabinejad M, McKendry DJ, Hong C-U, Kariyawasam SP (1995) Use of mineral trioxide aggregate for repair of furcal perforations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 79:756–762CrossRef

35.

Salman MA, Quinn F, Dermody J, Hussey D, Claffey N (1999) Histological evaluation of repair using a bioresorbable membrane beneath a resin-modified glass ionomer after mechanical furcation perforation in dogs’ teeth. J Endod 25:181–186CrossRefPubMed

36.

Mori GG, Teixeira LM, de Oliveira DL, Jacomini LM, da Silva SR (2014) Biocompatibility evaluation of Biodentine in subcutaneous tissue of rats. J Endod 40:1485–1488. https://doi.org/10.1016/j.joen.2014.02.027 CrossRefPubMed

37.

Silva GF, Bosso R, Ferino RV, Tanomaru-Filho M, Bernardi MI, Guerreiro-Tanomaru JM, Cerri PS (2014) Microparticulated and nanoparticulated zirconium oxide added to calcium silicate cement: evaluation of physicochemical and biological properties. J Biomed Mater Res A 102:4336–4345. https://doi.org/10.1002/jbm.a.35099 CrossRefPubMed

38.

Kang JY, Lee BN, Son HJ, Koh JT, Kang SS, Son HH, Chang HS, Hwang IN, Hwang YC, Oh WM (2013) Biocompatibility of mineral trioxide aggregate mixed with hydration accelerators. J Endod 39:497–500. https://doi.org/10.1016/j.joen.2012.11.037 CrossRefPubMed

39.

Torres FFE, Bosso-Martelo R, Espir CG, Cirelli JA, Guerreiro-Tanomaru JM, Tanomaru-Filho M (2017) Evaluation of physicochemical properties of root-end filling materials using conventional and Micro-CT tests. J Appl Oral Sci 25:374–380. https://doi.org/10.1590/1678-7757-2016-0454 CrossRefPubMedPubMedCentral

40.

Kaup M, Schäfer E, Dammaschke T (2015) An in vitro study of different material properties of Biodentine compared to ProRoot MTA. Head Face Med 11:16. https://doi.org/10.1186/s13005-015-0074-9 CrossRefPubMedPubMedCentral

41.

Williams DF (2008) On the mechanisms of biocompatibility. Biomaterials 29:2941–2953. https://doi.org/10.1016/j.biomaterials.2008.04.023 CrossRef

42.

Scheller J, Chalaris A, Schmidt-Arras D, Rose-John S (2011) The pro- and anti-inflammatory properties of the cytokine interleukin-6. Biochim Biophys Acta 1813:878–888. https://doi.org/10.1016/j.bbamcr.2011.01.034 CrossRef

43.

Azuma MM, Samuel RO, Gomes-Filho JE, Dezan-Junior E, Cintra LT (2014) The role of IL-6 on apical periodontitis: a systematic review. Int Endod J 47:615–621. https://doi.org/10.1111/iej.12196 CrossRefPubMed

44.

Yoshida Y, Tanaka T (2014) Interleukin 6 and rheumatoid arthritis. Biomed Res Int 2014:1–12. https://doi.org/10.1155/2014/698313 CrossRef

45.

Nakashima T, Hayashi M, Fukunaga T, Kurata K, Oh-Hora M, Feng JQ, Bonewald LF, Kodama T, Wutz A, Wagner EF, Penninger JM, Takayanagi H (2011) Evidence for osteocyte regulation of bone homeostasis through RANKL expression. Nat Med 17:1231–1234. https://doi.org/10.1038/nm.2452 CrossRef

46.

Florencio-Silva R, Sasso GR, Sasso-Cerri E, Simões MJ, Cerri PS (2015) Biology of bone tissue: structure, function, and factors that influence bone cells. Biomed Res Int 2015:421746. https://doi.org/10.1155/2015/421746 CrossRefPubMedPubMedCentral

47.

de Souza Faloni AP, Schoenmaker T, Azari A, Katchburian E, Cerri PS, de Vries TJ, Everts V (2011) Jaw and long bone marrows have a different osteoclastogenic potential. Calcif Tissue Int 88:63–74. https://doi.org/10.1007/s00223-010-9418-4 CrossRef

48.

de Souza Faloni AP, Sasso-Cerri E, Rocha FR, Katchburian E, Cerri PS (2012) Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats. J Anat 220:77–85. https://doi.org/10.1111/j.1469-7580.2011.01449.x CrossRef

49.

Costa F, Sousa Gomes P, Fernandes MH (2016) Osteogenic and angiogenic response to calcium silicate-based endodontic sealers. J Endod 42:113–119. https://doi.org/10.1016/j.joen.2015.09.020 CrossRefPubMed

50.

Laurent P, Camps J, About I (2012) Biodentine(TM) induces TGF-β1 release from human pulp cells and early dental pulp mineralization. Int Endod J 45:439–448. https://doi.org/10.1111/j.1365-2591.2011.01995.x CrossRefPubMed

51.

Loi F, Córdova LA, Pajarinen J, Lin TH, Yao Z, Goodman SB (2016) Inflammation, fracture and bone repair. Bone 86:119–130. https://doi.org/10.1016/j.bone.2016.02.020 CrossRefPubMedPubMedCentral

52.

Natale LC, Rodrigues MC, Xavier TA, Simões A, de Souza DN, Braga RR (2015) Ion release and mechanical properties of calcium silicate and calcium hydroxide materials used for pulp capping. Int Endod J 48:89–94. https://doi.org/10.1111/iej.12281 CrossRefPubMed

53.

Lucas CP, Viapiana R, Bosso-Martelo R, Guerreiro-Tanomaru JM, Camilleri J, Tanomaru-Filho M (2017) Physicochemical properties and dentin bond strength of a tricalcium silicate-based retrograde material. Braz Dent J 28:51–56. https://doi.org/10.1590/0103-6440201701135 CrossRefPubMed

54.

Han L, Okiji T (2013) Bioactivity evaluation of three calcium silicate-based endodontic materials. Int Endod J 46:808–814. https://doi.org/10.1111/iej.12062 CrossRefPubMed

55.

Silva EJ, Rosa TP, Herrera DR, Jacinto RC, Gomes BP, Zaia AA (2013) Evaluation of cytotoxicity and physicochemical properties of calcium silicate-based endodontic sealer MTA Fillapex. J Endod 39:274–277. https://doi.org/10.1016/j.joen.2012.06.030 CrossRefPubMed

56.

Michel A, Erber R, Frese C, Gehrig H, Saure D, Mente J (2017) In vitro evaluation of different dental materials used for the treatment of extensive cervical root defects using human periodontal cells. Clin Oral Investig 21:753–761. https://doi.org/10.1007/s00784-016-1830-3 CrossRefPubMed

Title: Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars
Authors: Tiago Silva da Fonseca
Guilherme F. Silva
Juliane M. Guerreiro-Tanomaru
Mateus Machado Delfino
Estela Sasso-Cerri
Mário Tanomaru-Filho
Paulo Sérgio Cerri
Publication date: 01-03-2019
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 3/2019
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2550-7

At a glance: The STEP trials

Springer Medicine

Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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