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Arthritis Research & Therapy
Published in: Arthritis Research & Therapy 1/2015

Open Access 01-12-2015 | Research article

Bach1 deficiency reduces severity of osteoarthritis through upregulation of heme oxygenase-1

Authors: Tsuyoshi Takada, Shigeru Miyaki, Hiroyuki Ishitobi, Yuya Hirai, Tomoyuki Nakasa, Kazuhiko Igarashi, Martin K. Lotz, Mitsuo Ochi

Published in: Arthritis Research & Therapy | Issue 1/2015

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Abstract

Introduction

BTB and CNC homology 1 (Bach1) is a transcriptional repressor of Heme oxygenase-1 (HO-1), which is cytoprotective through its antioxidant effects. The objective of this study was to define the role of Bach1 in cartilage homeostasis and osteoarthritis (OA) development using in vitro models and Bach1 -/- mice.

Methods

HO-1 expression in Bach1 -/- mice was analyzed by real-time PCR, immunohistochemistry and immunoblotting. Knee joints from Bach1 -/- and wild-type mice with age-related OA and surgically-induced OA were evaluated by OA scoring systems. Levels of autophagy proteins and superoxide dismutase 2 (SOD2) were determined by immunohistochemistry. The relationship between HO-1 and the protective effects for OA was determined in chondrocytes treated with small interfering RNA (siRNA) targeting HO-1 gene.

Results

HO-1 expression decreased with aging in articular cartilages and menisci of mouse knees. Bach1 -/- mice showed reduced severity of age-related OA and surgically-induced OA compared with wild-type mice. Microtubule-associated protein 1 light chain 3 (LC3), autophagy marker, and SOD2 were increased in articular cartilage of Bach1 -/- mice compared with wild-type mice. Interleukin-1β (IL-1β) induced a significant increase in Adamts-5 in wild-type chondrocytes but not in Bach1-/- chondrocytes. The expression of SOD2 and the suppression of apoptosis in Bach1 -/- chondrocytes were mediated by HO-1.

Conclusions

Bach1 deficiency reduces the severity of OA-like changes. This may be due to maintenance of cartilage homeostasis and joint health by antioxidant effects through HO-1 and downregulation of extracellular matrix degrading enzymes. These results suggest that inactivation of Bach1 is a novel target and signaling pathway in OA prevention.
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Metadata
Title
Bach1 deficiency reduces severity of osteoarthritis through upregulation of heme oxygenase-1
Authors
Tsuyoshi Takada
Shigeru Miyaki
Hiroyuki Ishitobi
Yuya Hirai
Tomoyuki Nakasa
Kazuhiko Igarashi
Martin K. Lotz
Mitsuo Ochi
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Arthritis Research & Therapy / Issue 1/2015
Electronic ISSN: 1478-6362
DOI
https://doi.org/10.1186/s13075-015-0792-1

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