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01-06-2020 | Autism Spectrum Disorder | Original Paper

Disregulation of Autophagy in the Transgenerational Cc2d1a Mouse Model of Autism

Authors: Halime Dana, Keziban Korkmaz Bayramov, Nesrin Delibaşı, Reyhan Tahtasakal, Ruslan Bayramov, Zuhal Hamurcu, Elif Funda Sener

Published in: NeuroMolecular Medicine | Issue 2/2020

Abstract

Autism spectrum disorder (ASD) is a heterogeneously childhood neurodevelopmental disorder, believed to be under development of various genetic and environmental factors. Autophagy and related pathways have also been implicated in the etiology of ASD. We aimed to investigate autophagic markers by generating the transgenerational inheritance of ASD-like behaviors in the Cc2d1a animal model of ASD. Cc2d1a (+/−) mouse model of ASD was built in two different groups by following three generations. After behavior test, bilateral hippocampus was sliced. Western Blot assay and quantitative real-time polymerase chain reaction (QRT-PCR) were used for measurement of LC3 and Beclin-1 as key regulators of autophagy. All of the animal and laboratory studies were conducted in the Erciyes University Genome and Stem Cell Center (GENKOK). Significant LC3 and Beclin-1 mRNA expression levels were observed in mouse hippocampus between groups and generations. Western blot confirmed the changes of the proteins in the hippocampus. LC3 expressions were increased for females and decreased for males compared to the control group. Beclin-1 expression levels were found to be significantly decreased in males and females compared to controls. This study could help explain a new pathway of autophagy in ASD mouse models. Future animal studies need to investigate sex differences in mouse modeling autism-relevant genes like CC2D1A. We anticipate our results to be a starting point for more comprehensive autophagy studies in this mouse model of ASD.

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Title: Disregulation of Autophagy in the Transgenerational Cc2d1a Mouse Model of Autism
Authors: Halime Dana
Keziban Korkmaz Bayramov
Nesrin Delibaşı
Reyhan Tahtasakal
Ruslan Bayramov
Zuhal Hamurcu
Elif Funda Sener
Publication date: 01-06-2020
Publisher: Springer US
Keywords: Autism Spectrum Disorder
Polymerase Chain Reaction
Published in: NeuroMolecular Medicine / Issue 2/2020
Print ISSN: 1535-1084
Electronic ISSN: 1559-1174
DOI: https://doi.org/10.1007/s12017-019-08579-x

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Disregulation of Autophagy in the Transgenerational Cc2d1a Mouse Model of Autism

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