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Journal of Assisted Reproduction and Genetics

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01-02-2020 | Assisted Reproduction | Reproductive Physiology and Disease

The lipidome of endometrial fluid differs between implantative and non-implantative IVF cycles

Authors: Roberto Matorras, Ibon Martinez-Arranz, Enara Arretxe, Marta Iruarrizaga-Lejarreta, Blanca Corral, Jone Ibañez-Perez, Antonia Exposito, Begoña Prieto, Felix Elortza, Cristina Alonso

Published in: Journal of Assisted Reproduction and Genetics | Issue 2/2020

Abstract

Objective

To characterize the most relevant changes in the lipidome of endometrial fluid aspirate (EFA) in non-implantative cycles.

Design

Lipidomics in a prospective cohort study.

Settings

Reproductive unit of a university hospital.

Patients

Twenty-nine women undergoing an IVF cycle. Fifteen achieved pregnancy and 14 did not.

Intervention

Endometrial fluid aspiration immediately before performing embryo transfer.

Main outcome measures

Clinical pregnancy rate and lipidomic profiles obtained on an ultra-high performance liquid chromatography coupled to time-of-flight mass spectrometry (UHPLC-ToF-MS)-based analytical platform.

Results

The comparative analysis of the lipidomic patterns of endometrial fluid in implantative and non-implantative IVF cycles revealed eight altered metabolites: seven glycerophospholipids and an omega-6 polyunsaturated fatty acid. Then, women with a non-implantative cycle were accurately classified with a support vector machine algorithm including these eight lipid metabolites. The diagnostic performances of the algorithm showed an area under the receiver operating characteristic curve, sensitivity, specificity, and accuracy of 0.893 ± 0.07, 85.7%, 80.0%, and 82.8%, respectively.

Conclusion

A predictive lipidomic signature linked to the implantative status of the endometrial fluid has been found.

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Title: The lipidome of endometrial fluid differs between implantative and non-implantative IVF cycles
Authors: Roberto Matorras
Ibon Martinez-Arranz
Enara Arretxe
Marta Iruarrizaga-Lejarreta
Blanca Corral
Jone Ibañez-Perez
Antonia Exposito
Begoña Prieto
Felix Elortza
Cristina Alonso
Publication date: 01-02-2020
Publisher: Springer US
Keyword: Assisted Reproduction
Published in: Journal of Assisted Reproduction and Genetics / Issue 2/2020
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-019-01670-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The lipidome of endometrial fluid differs between implantative and non-implantative IVF cycles

Abstract

Objective

Design

Settings

Patients

Intervention

Main outcome measures

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Design

Settings

Patients

Intervention

Main outcome measures

Results

Conclusion

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