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Journal of Assisted Reproduction and Genetics

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01-02-2020 | Infertility | Gamete Biology

Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure

Authors: Minhao Hu, Lejun Li, Shuyuan Liu, Yiyun Lou, Liya Wang, Fang Le, Hongping Li, Qijing Wang, Hangying Lou, Ning Wang, Fan Jin

Published in: Journal of Assisted Reproduction and Genetics | Issue 2/2020

Abstract

Purpose

To assess testicular mRNA and protein expression levels of MRE11 and RAD50 in human azoospermia patients.

Methods

Patients diagnosed with maturation arrest at the spermatocyte stage (MA) and Sertoli cell-only syndrome (SCOS) were recruited through diagnostic testicular biopsy. Patients with normal spermatogenesis were studied as controls. In addition, knockdown of MRE11 and RAD50 was performed in GC-2spd(ts) cells to investigate their roles in cellular proliferation and apoptosis.

Results

mRNA and protein expression levels of MRE11 and RAD50 were measured using quantitative polymerase chain reaction, western blotting, and immunohistochemistry, respectively. Knockdown of both MRE11 and RAD50 utilized transfection with small interfering RNAs.

Conclusion

Our findings demonstrated altered expression levels of MRE11 and RAD50 in human testes with MA and SCOS, and showed that these alterations might be associated with impaired spermatogenesis. These results offer valuable new perspectives into the molecular mechanisms of male infertility.

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Title: Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure
Authors: Minhao Hu
Lejun Li
Shuyuan Liu
Yiyun Lou
Liya Wang
Fang Le
Hongping Li
Qijing Wang
Hangying Lou
Ning Wang
Fan Jin
Publication date: 01-02-2020
Publisher: Springer US
Keywords: Infertility
Infertility
Published in: Journal of Assisted Reproduction and Genetics / Issue 2/2020
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-019-01686-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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