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Open Access 01-02-2021 | Coronavirus | Case Report

Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs

Authors: Marianna Theresia Traugott, Wolfgang Hoepler, Tamara Seitz, Sebastian Baumgartner, Mario Karolyi, Erich Pawelka, Emanuela Friese, Stephanie Neuhold, Hasan Kelani, Florian Thalhammer, Alexander Zoufaly, Hermann Laferl, Judith Helene Aberle, Christoph Wenisch, Elisabeth Puchhammer-Stöckl, Karin Stiasny, Stephan Walter Aberle, Lukas Weseslindtner

Published in: Infection | Issue 1/2021

Abstract

We report of two cases of progressed COVID-19 with negative PCR tests from nasopharyngeal swabs, in whom diagnosis was made by different antibody assays, including a lateral flow rapid test and multiple commercial ELISAs, finally confirmed by comprehensive serological assays. These cases highlight that commercial ELISAs and even rapid tests might significantly aid the diagnosis of COVID-19, particularly, if a combination of serological assays is used with a specific clinical question, in severely ill patients after seroconversion and when comprehensive serological methods are used for confirmation.

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Title: Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
Authors: Marianna Theresia Traugott
Wolfgang Hoepler
Tamara Seitz
Sebastian Baumgartner
Mario Karolyi
Erich Pawelka
Emanuela Friese
Stephanie Neuhold
Hasan Kelani
Florian Thalhammer
Alexander Zoufaly
Hermann Laferl
Judith Helene Aberle
Christoph Wenisch
Elisabeth Puchhammer-Stöckl
Karin Stiasny
Stephan Walter Aberle
Lukas Weseslindtner
Publication date: 01-02-2021
Publisher: Springer Berlin Heidelberg
Keywords: Coronavirus
SARS-CoV-2
COVID-19
Polymerase Chain Reaction
Published in: Infection / Issue 1/2021
Print ISSN: 0300-8126
Electronic ISSN: 1439-0973
DOI: https://doi.org/10.1007/s15010-020-01497-2

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