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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 3/2019

01-06-2019 | Research Article

Apoptotic PET Imaging of Rat Pulmonary Fibrosis with Small-Molecule Radiotracer

Authors: Ying Xiong, Dahong Nie, Shaoyu Liu, Hui Ma, Shu Su, Aixia Sun, Jing Zhao, Zhanwen Zhang, Xianhong Xiang, Ganghua Tang

Published in: Molecular Imaging and Biology | Issue 3/2019

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Abstract

Purpose

The purpose of this study was to assess the potential utility of small-molecule apoptotic radiotracer, 2-(5-[18F]fluoropentyl)-2-methyl malonic acid ([18F]ML-10), for positron emission tomography (PET)/computed tomography (CT) monitoring the progression of pulmonary fibrosis in a rat model.

Procedures

Male Sprague-Dawley rats were used to establish a rat model of pulmonary fibrosis by means of bleomycin (BLM) administration; control rats received saline (n = 12 per group). PET/CT with [18F]ML-10 and 2-deoxy-2-[18F]fluoro-d-glucose ([18F]FDG) was performed in two groups at different stages of pulmonary fibrosis. The fibrotic response and the cell apoptosis were assessed with histologic examination. Differences in the apoptosis rate, fibrotic activity, and the lung uptake of [18F]ML-10 and [18F]FDG between two groups were determined with Student t test.

Results

Compared with control group, BLM group showed a higher lung uptake of [18F]ML-10 at all imaging time points (all P < 0.001). During the fibrotic phase of this disease model (days 21 and 28), the lung uptake of [18F]ML-10 was higher than that of [18F]FDG in the BLM group (all P < 0.001). Moreover, accumulation of [18F]ML-10 in the lung tissues increased in proportion to the apoptosis rate (R2 = 0.9863, P < 0.0001) and fibrotic activity (R2 = 0.9631, P < 0.0001) of rat pulmonary fibrosis. Conversely, no correlation between [18F]FDG uptake and fibrotic activity was found.

Conclusions

[18F]ML-10 PET/CT enabled monitoring the progression of rat pulmonary fibrosis, whereas [18F]FDG PET/CT could not. Implications for noninvasive diagnosis of pulmonary fibrosis, assessment of fibrotic activity, and evaluation of antifibrotic therapy are expected.
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Metadata
Title
Apoptotic PET Imaging of Rat Pulmonary Fibrosis with Small-Molecule Radiotracer
Authors
Ying Xiong
Dahong Nie
Shaoyu Liu
Hui Ma
Shu Su
Aixia Sun
Jing Zhao
Zhanwen Zhang
Xianhong Xiang
Ganghua Tang
Publication date
01-06-2019
Publisher
Springer International Publishing
Published in
Molecular Imaging and Biology / Issue 3/2019
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-018-1242-7

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