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European Archives of Paediatric Dentistry

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14-06-2023 | Antibiotic | Original Scientific Article

Efficacy of propolis and triple antibiotic paste as intra-canal medicaments for revascularisation of immature teeth in dogs: a comparative study

Authors: S. Moradi, S. Moushekhian, E. Najafi, H. S. Sedigh, S. Navabi

Published in: European Archives of Paediatric Dentistry | Issue 3/2023

Abstract

Aim

Revascularisation is a therapeutic approach for treatment of immature teeth with necrotic pulp. The conventional protocol includes application of triple antibiotic paste (TAP). The present study aimed to compare the efficacy of propolis and TAP as intra-canal medicaments for revascularisation of immature teeth in dogs.

Methodology

This study was conducted on 20 immature (open-apex) canine teeth in mixed breaded dogs. First, the teeth were exposed to oral environment, followed by intra-canal cleaning and shaping 2 weeks later.. The teeth were divided into two groups. The TAP group received paste comprising of ciprofloxacin, metronidazole, and minocycline (at a concentration of 100 µg/mL), while propolis (15% w/v) was used in the other group. The revascularisation procedure was done by means of sodium hypochlorite, EDTA and distilled water as final irrigant. After dehumidifying and induction of bleeding, mineral trioxide aggregate (MTA) was applied At 6-month follow-up, radiographs were evaluated by two observers regarding root length and thickness, intra-canal calcification, periapical lesions, and apex formation. Data were analysed by the Chi-square and Fisher’s exact tests.

Results

There was no significant difference between the TAP and propolis groups in root length or root thickness increase, calcification, related lesions, or apex formation (P > 0.05).

Conclusion

Findings of the present study in experimental animals revealed that the efficacy of propolis as an intra-canal medicament is comparable to that of triple antibiotic paste for revascularisation therapy.

Albuquerque MT, Nagata J, Bottino MC. Antimicrobial efficacy of triple antibiotic–eluting polymer nanofibers against multispecies biofilm. J Endod. 2017;43(9):S51–6. https://doi.org/10.1016/j.joen.2017.06.009.CrossRefPubMedPubMedCentral

Al-Shaher A, Wallace J, Agarwal S, Bretz W, Baugh D. Effect of propolis on human fibroblasts from the pulp and periodontal ligament. J Endod. 2004;30(5):359–61. https://doi.org/10.1097/00004770-200405000-00012.CrossRefPubMed

Banchs F, Trope M. Revascularization of immature permanent teeth with apical periodontitis: new treatment protocol? J Endod. 2004;30(4):196–200. https://doi.org/10.1097/00004770-200404000-00003.CrossRefPubMed

Bottino MC, Albuquerque MT, Azabi A, Münchow EA, Spolnik KJ, Nör JE, Edwards PC. A novel patient-specific three-dimensional drug delivery construct for regenerative endodontics. J Biomed Mater Res B Appl Biomater. 2019;107(5):1576–86. https://doi.org/10.1002/jbm.b.34250.CrossRefPubMed

Bueno-Silva B, Marsola A, Ikegaki M, Alencar SM, Rosalen PL. The effect of seasons on Brazilian red propolis and its botanical source: chemical composition and antibacterial activity. Nat Prod Res. 2017;31(11):1318–24. https://doi.org/10.1080/14786419.2016.1239088.CrossRefPubMed

Chandwani ND, Maurya N, Nikhade P, Chandwani J. Comparative evaluation of antimicrobial efficacy of calcium hydroxide, triple antibiotic paste and bromelain against Enterococcus faecalis: an in vitro study. J Conserv Dent. 2022;25(1):63–7. https://doi.org/10.4103/jcd.jcd_461_21.CrossRefPubMedPubMedCentral

Chuensombat S, Khemaleelakul S, Chattipakorn S, Srisuwan T. Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: an in vitro study. J Endod. 2013;39(6):813–9. https://doi.org/10.1016/j.joen.2012.11.041.CrossRefPubMed

Conde MCM, Chisini LA, Sarkis-Onofre R, Schuch HS, Nör JE, Demarco FF. A scoping review of root canal revascularization: relevant aspects for clinical success and tissue formation. Int Endod J. 2017;50(9):860–74. https://doi.org/10.1111/iej.12711.CrossRefPubMed

Da Silva Magalhães K, Kuerten-Gil AC, Goulart TS, Schuldt DPV, Coelho BS, Figueiredo DR, Garcia L, de Almeida J. Efficacy of disinfection procedures performed prior to regenerative endodontic therapy: an integrative review. Aust Endod J. 2022;00:1–10. https://doi.org/10.1111/aej.12670.CrossRef

Dianat O, Mashhadi-Abas F, Paymanpour P, Eghbal MJ, Haddadpour S, Bahrololumi N. Endodontic repair in immature dogs’ teeth with apical periodontitis: blood clot vs plasma rich in growth factors scaffold. Dent Traumatol. 2017;33(2):84–90. https://doi.org/10.1111/edt.12306.CrossRefPubMed

Ding RY, Cheung GS, Chen J, Yin XZ, Wang QQ, Zhang CF. Pulp revascularization of immature teeth with apical periodontitis: a clinical study. J Endod. 2009;35(5):745–9. https://doi.org/10.1016/j.joen.2009.02.009.CrossRefPubMed

Dubey N, Xu J, Zhang Z, Nör JE, Bottino MC. Comparative evaluation of the cytotoxic and angiogenic effects of minocycline and clindamycin: an in vitro study. J Endod. 2019;45(7):882–9. https://doi.org/10.1016/j.joen.2019.04.007.CrossRefPubMedPubMedCentral

Eickholz P, Kim TS, Bürklin T, Schacher B, Renggli HH, Schaecken MT, Holle R, Kübler A, Ratka-Krüger P. Non-surgical periodontal therapy with adjunctive topical doxycycline: a double-blind randomized controlled multicenter study. J Clin Periodontol. 2002;29(2):108–17. https://doi.org/10.1034/j.1600-051x.2002.290204.x.CrossRefPubMed

El-Guendouz S, Lyoussi B, Miguel MG. Insight on propolis from mediterranean countries: chemical composition, biological activities and application fields. Chem Biodivers. 2019;16(7): e1900094. https://doi.org/10.1002/cbdv.201900094.CrossRefPubMed

El-Tayeb MM, Abu-Seida AM, El Ashry SH, El-Hady SA. Evaluation of antibacterial activity of propolis on regenerative potential of necrotic immature permanent teeth in dogs. BMC Oral Health. 2019;19(1):174. https://doi.org/10.1186/s12903-019-0835-0.CrossRefPubMedPubMedCentral

Farges J-C, Alliot-Licht B, Renard E, Ducret M, Gaudin A, Smith AJ, Cooper PR. Dental pulp defence and repair mechanisms in dental caries. Mediat Inflamm. 2015. https://doi.org/10.1155/2015/230251.CrossRef

Galler KM, Buchalla W, Hiller K-A, Federlin M, Eidt A, Schiefersteiner M, Schmalz G. Influence of root canal disinfectants on growth factor release from dentin. J Endod. 2015;41(3):363–8. https://doi.org/10.1016/j.joen.2014.11.021.CrossRefPubMed

Hargreaves KM, Diogenes A, Teixeira FB. Treatment options: biological basis of regenerative endodontic procedures. J Endod. 2013;39(3 Suppl):S30-43. https://doi.org/10.1016/j.joen.2012.11.025.CrossRefPubMedPubMedCentral

Hayacibara MF, Koo H, Rosalen PL, Duarte S, Franco EM, Bowen WH, Ikegaki M, Cury JA. In vitro and in vivo effects of isolated fractions of Brazilian propolis on caries development. J Ethnopharmacol. 2005;101(1):110–5. https://doi.org/10.1016/j.jep.2005.04.001.CrossRefPubMed

Hoshino E, Kurihara-Ando N, Sato I, Uematsu H, Sato M, Kota K, Iwaku M. In-vitro antibacterial susceptibility of bacteria taken from infected root dentine to a mixture of ciprofloxacin, metronidazole and minocycline. Int Endod J. 1996;29(2):125–30. https://doi.org/10.1111/j.1365-2591.1996.tb01173.x.CrossRefPubMed

Isik S, Karakaya G, Erkin G, Kalyoncu A. Multidrug-induced erythema multiforme. J Investig Allergol Clin Immunol. 2007;17(3):196.PubMed

Iwaya SI, Ikawa M, Kubota M. Revascularization of an immature permanent tooth with apical periodontitis and sinus tract. Dent Traumatol. 2001;17(4):185–7. https://doi.org/10.1034/j.1600-9657.2001.017004185.x.CrossRefPubMed

Kim JH, Kim Y, Shin SJ, Park JW, Jung IY. Tooth discoloration of immature permanent incisor associated with triple antibiotic therapy: a case report. J Endod. 2010;36(6):1086–91. https://doi.org/10.1016/j.joen.2010.03.031.CrossRefPubMed

Lillygrace E, Kethineni B, Puppala R, Raichurkar HK, Ambati S, Saikiran KV. Antimicrobial efficacy of triple antibiotic paste and propolis as an intracanal medicament in young permanent teeth: an in vivo study. Int J Clin Pediatr Dent. 2021;14(2):243–8. https://doi.org/10.5005/jp-journals-10005-1944.CrossRefPubMedPubMedCentral

Londero de L, Pagliarin CM, Felippe MC, Felippe WT, Danesi CC, Barletta FB. Histologic analysis of the influence of a gelatin-based scaffold in the repair of immature dog teeth subjected to regenerative endodontic treatment. J Endod. 2015;41(10):1619–25. https://doi.org/10.1016/j.joen.2015.01.033.CrossRef

Madhubala MM, Srinivasan N, Ahamed S. Comparative evaluation of propolis and triantibiotic mixture as an intracanal medicament against Enterococcus faecalis. J Endod. 2011;37(9):1287–9. https://doi.org/10.1016/j.joen.2011.05.028.CrossRefPubMed

Madsen JT, Thormann J, Kerre S, Andersen KE, Goossens A. Allergic contact dermatitis to topical metronidazole-3 cases. Contact Dermat. 2007;56(6):364–5. https://doi.org/10.1111/j.1600-0536.2006.01064.x.CrossRef

Mohan SP, Ramalingam M. Dental pulp stem cells in neuroregeneration. J Pharm Bioallied Sci. 2020;12(Suppl 1):S60-s66. https://doi.org/10.4103/jpbs.JPBS_229_20.CrossRefPubMedPubMedCentral

Nashaat Y, Sabry H, Hassan SA. Evaluation of the cytotoxicity and apoptotic effect of nano triple antibiotic paste with nano anti-inflammatory drug as an intracanal medicament. Eur Endod J. 2021;6(1):82–9. https://doi.org/10.14744/eej.2020.29292.CrossRefPubMedPubMedCentral

Nosrat A, Seifi A, Asgary S. Regenerative endodontic treatment (revascularization) for necrotic immature permanent molars: a review and report of two cases with a new biomaterial. J Endod. 2011;37(4):562–7. https://doi.org/10.1016/j.joen.2011.01.011.CrossRefPubMed

Ordinola Zapata R, Bramante CM, Minotti PG, Cavenago BC, Garcia RB, Bernardineli N, Jaramillo DE, Hungaro Duarte MA. Antimicrobial activity of triantibiotic paste, 2% chlorhexidine gel, and calcium hydroxide on an intraoral-infected dentin biofilm model. J Endod. 2013;39(1):115–8. https://doi.org/10.1016/j.joen.2012.10.004.CrossRefPubMed

Pagliarin CML, Londero CDLD, Felippe MCS, Felippe WT, Danesi CC, Barletta FB. Tissue characterization following revascularization of immature dog teeth using different disinfection pastes. Braz Oral Res. 2016. https://doi.org/10.1590/1807-3107BOR-2016.vol30.0074.CrossRefPubMed

Ribeiro JS, Münchow EA, Ferreira Bordini EA, de Oliveira da Rosa WL, Bottino MC. Antimicrobial therapeutics in regenerative endodontics: a scoping review. J Endod. 2020;46(9):S115-s127. https://doi.org/10.1016/j.joen.2020.06.032.CrossRefPubMedPubMedCentral

Sachdeva GS, Sachdeva LT, Goel M, Bala S. Regenerative endodontic treatment of an immature tooth with a necrotic pulp and apical periodontitis using platelet-rich plasma (PRP) and mineral trioxide aggregate (MTA): a case report. Int Endod J. 2015;48(9):902–10. https://doi.org/10.1111/iej.12407.CrossRefPubMed

Saoud TM, Zaazou A, Nabil A, Moussa S, Lin LM, Gibbs JL. Clinical and radiographic outcomes of traumatized immature permanent necrotic teeth after revascularization/revitalization therapy. J Endod. 2014;40(12):1946–52. https://doi.org/10.1016/j.joen.2014.08.023.CrossRefPubMedPubMedCentral

Sato I, Ando-Kurihara N, Kota K, Iwaku M, Hoshino E. Sterilization of infected root-canal dentine by topical application of a mixture of ciprofloxacin, metronidazole and minocycline in situ. Int Endod J. 1996;29(2):118–24. https://doi.org/10.1111/j.1365-2591.1996.tb01172.x.CrossRefPubMed

Slots J. Selection of antimicrobial agents in periodontal therapy. J Periodont Res. 2002;37(5):389–98. https://doi.org/10.1034/j.1600-0765.2002.00004.x.CrossRef

Tamhankar K, Dhaded NS, Kore P, Nagmoti JM, Hugar SM, Patil AC. Comparative evaluation of efficacy of calcium hydroxide, propolis, and glycyrrhiza glabra as intracanal medicaments in root canal treatment. J Contemp Dent Pract. 2021;22(6):707–12. https://doi.org/10.5005/jp-journals-10024-3106.CrossRefPubMed

Tawfik H, Abu-Seida A, Hashem A, Nagy M. Regenerative potential following revascularization of immature permanent teeth with necrotic pulps. Int Endod J. 2013;46(10):910–22. https://doi.org/10.1111/iej.12079.CrossRefPubMed

Torneck CD, Smith JS, Grindall P. Biologic effects of endodontic procedures on developing incisor teeth. II. Effect of pulp injury and oral contamination. Oral Surg Oral Med Oral Pathol. 1973;35(3):378–88. https://doi.org/10.1016/0030-4220(73)90076-5.CrossRefPubMed

Viuda-Martos M, Ruiz-Navajas Y, Fernandez-Lopez J, Perez-Alvarez JA. Functional properties of honey, propolis, and royal jelly. J Food Sci. 2008;73(9):R117-124. https://doi.org/10.1111/j.1750-3841.2008.00966.x.CrossRefPubMed

Wang X, Thibodeau B, Trope M, Lin LM, Huang GT. Histologic characterization of regenerated tissues in canal space after the revitalization/revascularization procedure of immature dog teeth with apical periodontitis. J Endod. 2010;36(1):56–63. https://doi.org/10.1016/j.joen.2009.09.039.CrossRefPubMed

Wigler R, Kaufman AY, Lin S, Steinbock N, Hazan-Molina H, Torneck CD. Revascularization: a treatment for permanent teeth with necrotic pulp and incomplete root development. J Endod. 2013;39(3):319–26. https://doi.org/10.1016/j.joen.2012.11.014.CrossRefPubMed

Yamauchi N, Yamauchi S, Nagaoka H, Duggan D, Zhong S, Lee SM, Teixeira FB, Yamauchi M. Tissue engineering strategies for immature teeth with apical periodontitis. J Endod. 2011;37(3):390–7. https://doi.org/10.1016/j.joen.2010.11.010.CrossRefPubMed

Yassen GH, Chu TM, Eckert G, Platt JA. Effect of medicaments used in endodontic regeneration technique on the chemical structure of human immature radicular dentin: an in vitro study. J Endod. 2013;39(2):269–73. https://doi.org/10.1016/j.joen.2012.09.020.CrossRefPubMed

Zancan RF, Cavenago BC, Oda DF, Bramante CM, Andrade FB, Duarte MAH. Antimicrobial activity and physicochemical properties of antibiotic pastes used in regenerative endodontics. Braz Dent J. 2019;30(6):536–41. https://doi.org/10.1590/0103-6440201902613.CrossRefPubMed

ZareJahromi M, Ranjbarian P, Shiravi S. Cytotoxicity evaluation of Iranian propolis and calcium hydroxide on dental pulp fibroblasts. J Dent Res Dent Clin Dent Prospects. 2014;8(3):130–3. https://doi.org/10.5681/joddd.2014.024.CrossRef

Title: Efficacy of propolis and triple antibiotic paste as intra-canal medicaments for revascularisation of immature teeth in dogs: a comparative study
Authors: S. Moradi
S. Moushekhian
E. Najafi
H. S. Sedigh
S. Navabi
Publication date: 14-06-2023
Publisher: Springer Berlin Heidelberg
Keywords: Antibiotic
Minocycline
Metronidazole
Ciprofloxacin
Published in: European Archives of Paediatric Dentistry / Issue 3/2023
Print ISSN: 1818-6300
Electronic ISSN: 1996-9805
DOI: https://doi.org/10.1007/s40368-023-00806-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Efficacy of propolis and triple antibiotic paste as intra-canal medicaments for revascularisation of immature teeth in dogs: a comparative study

Abstract

Aim

Methodology

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Aim

Methodology

Results

Conclusion

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