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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2019

Open Access 01-12-2019 | Research article

Antibacterial activities of triterpenoidal compounds isolated from Calothamnus quadrifidus leaves

Authors: H. A. Ibrahim, M. R. Elgindi, R. R. Ibrahim, D. G. El-Hosari

Published in: BMC Complementary Medicine and Therapies | Issue 1/2019

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Abstract

Background

Calothamnus quadrifidus R.Br has many traditional uses and there are few reports about its chemical and biological activities. So our aim is to isolate the triterpenoidal compounds from dichloromethane fraction (DCMF) of Calothamnus quadrifidus R.Br leaves and in addition to evaluate the antibacterial activity of the isolated compounds.

Methods

DCMF of C. quadrifidus leaves was subjected to different chromatographic techniques to isolate pure triterpenoidal compounds which were identified using different chemical and spectroscopic techniques. Antibacterial activities of the isolated compounds were evaluated using agar well diffusion method while minimum inhibitory concentration was assessed by microtiter plate assay method.

Results

Five compounds were isolated and they were betulinic acid (1), ursolic acid (2), 3-acetyl-23-hydroxy betulinic acid (3), 2,23-dihydroxy betulinic acid (4) and 2,21,23-trihydroxy betulinic acid (5) were isolated from DCMF of C. quadrifidus leaves for the first time. Compounds 4 and 5 showed strong antibacterial activity against S. typhimurium while compound 4, 5 and 3, 4 exhibits moderate effect against E.coli and S. aureus respectively.

Conclusion

Pure triterpenoidal compounds isolated from C. quadrifidus leaves showed antibacterial activities in different strengths.
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Metadata
Title
Antibacterial activities of triterpenoidal compounds isolated from Calothamnus quadrifidus leaves
Authors
H. A. Ibrahim
M. R. Elgindi
R. R. Ibrahim
D. G. El-Hosari
Publication date
01-12-2019
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2019
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-019-2512-x

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