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Journal of Clinical Immunology
Published in: Journal of Clinical Immunology 1/2010

Open Access 01-05-2010

Analytical and Functional Aspects of Antibody Sialylation

Authors: Johannes Stadlmann, Martin Pabst, Friedrich Altmann

Published in: Journal of Clinical Immunology | Special Issue 1/2010

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Abstract

Materials and methods

This review focuses on the role of antibody sialylation and methods for its quantitation. The recent attribution of the anti-inflammatory activity of IgG to the sialylation of its glycans in the Fc region has raised interest in the fine structure and analysis of the glycans. The anti-inflammatory fraction of intravenous IgG could be isolated with the Sambucus nigra lectin. Experimental strategies for the assessment of antibody sialylation are discussed.

Results

Thorough analysis of the lectin-binding fraction revealed that the antibody Fc region only binds to S. nigra lectin when two sialic acids are present, whereas for other glycoprotein ligands, one sialic acid appears sufficient.
Literature
1.
Jefferis R. Glycosylation as a strategy to improve antibody-based therapeutics. Nat Rev Drug Discov. 2009;8:226–34.CrossRefPubMed
2.
Raju TS. Terminal sugars of Fc glycans influence antibody effector functions of IgGs. Curr Opin Immunol. 2008;20:471–8.CrossRefPubMed
3.
Anthony RM, Nimmerjahn F, Ashline DJ, Reinhold VN, Paulson JC, Ravetch JV. Recapitulation of IVIG anti-inflammatory activity with a recombinant IgG Fc. Science. 2008;320:373–6.CrossRefPubMed
4.
Kaneko Y, Nimmerjahn F, Ravetch JV. Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation. Science. 2006;313:670–3.CrossRefPubMed
5.
Wuhrer M, Porcelijn L, Kapur R, Koeleman CA, Deelder A, de Haas M, et al. Regulated glycosylation patterns of IgG during alloimmune responses against human platelet antigens. J Proteome Res. 2009;8:450–6.CrossRefPubMed
6.
Huhn C, Selman MH, Ruhaak LR, Deelder AM, Wuhrer M. IgG glycosylation analysis. Proteomics. 2009;9:882–913.CrossRefPubMed
7.
Stadlmann J, Pabst M, Kolarich D, Kunert R, Altmann F. Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides. Proteomics. 2008;8:2571–858.CrossRef
8.
Wuhrer M, Stam JC, van de Geijn FE, Koeleman CA, Verrips CT, Dolhain RJ, et al. Glycosylation profiling of immunoglobulin G (IgG) subclasses from human serum. Proteomics. 2007;7:4070–81.CrossRefPubMed
9.
Beck A, Bussat MC, Zorn N, Robillard V, Klinguer-Hamour C, Chenu S, et al. Characterization by liquid chromatography combined with mass spectrometry of monoclonal anti-IGF-1 receptor antibodies produced in CHO and NS0 cells. J Chromatogr B Analyt Technol Biomed Life Sci. 2005;819:203–18.CrossRefPubMed
10.
Sinha S, Pipes G, Topp EM, Bondarenko PV, Treuheit MJ, Gadgil HS. Comparison of LC and LC/MS methods for quantifying N-glycosylation in recombinant IgGs. J Am Soc Mass Spectrom. 2008;19:1643–54.CrossRefPubMed
11.
Wada Y, Azadi P, Costello CE, Dell A, Dwek RA, Geyer H, et al. Comparison of the methods for profiling glycoprotein glycans—HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study. Glycobiology. 2007;17:411–22.CrossRefPubMed
12.
Olivova P, Chen W, Chakraborty AB, Gebler JC. Determination of N-glycosylation sites and site heterogeneity in a monoclonal antibody by electrospray quadrupole ion-mobility time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2008;22:29–40.CrossRefPubMed
13.
Mimura Y, Ashton PR, Takahashi N, Harvey DJ, Jefferis R. Contrasting glycosylation profiles between Fab and Fc of a human IgG protein studied by electrospray ionization mass spectrometry. J Immunol Methods. 2007;326:116–26.CrossRefPubMed
14.
Mimura Y, Ghirlando R, Sondermann P, Lund J, Jefferis R. The molecular specificity of IgG-Fc interactions with Fc gamma receptors. Adv Exp Med Biol. 2001;495:49–53.PubMed
15.
Pabst M, Bondili JS, Stadlmann J, Mach L, Altmann F. Mass + retention time = structure: a strategy for the analysis of N-glycans by carbon LC-ESI-MS and its application to fibrin N-glycans. Anal Chem. 2007;79:5051–7.CrossRefPubMed
16.
Pabst M, Kolarich D, Pöltl G, Dalik T, Lubec G, Hofinger A, et al. Comparison of fluorescent labels for oligosaccharides and introduction of a new postlabeling purification method. Anal Biochem. 2009;384:263–73.CrossRefPubMed
17.
Thobhani S, Yuen CT, Bailey MJ, Jones C. Identification and quantification of N-linked oligosaccharides released from glycoproteins: an inter-laboratory study. Glycobiology. 2009;19:201–11.CrossRefPubMed
18.
Guile GR, Rudd PM, Wing DR, Prime SB, Dwek RA. A rapid high-resolution high-performance liquid chromatographic method for separating glycan mixtures and analyzing oligosaccharide profiles. Anal Biochem. 1996;240:210–26.CrossRefPubMed
19.
Pabst M, Altmann F. Influence of electrosorption, solvent, temperature, and ion polarity on the performance of LC-ESI-MS using graphitic carbon for acidic oligosaccharides. Anal Chem. 2008;80:7534–42.CrossRefPubMed
20.
Holland M, Yagi H, Takahashi N, Kato K, Savage CO, Goodall DM, et al. Differential glycosylation of polyclonal IgG. IgG-Fc and IgG-Fab isolated from the sera of patients with ANCA-associated systemic vasculitis Biochim Biophys Acta. 2006;1760:669–77.
21.
Jefferis R, Lund J, Mizutani H, Nakagawa H, Kawazoe Y, Arata Y, et al. A comparative study of the N-linked oligosaccharide structures of human IgG subclass proteins. Biochem J. 1990;268:529–37.PubMed
22.
Stadlmann J, Weber A, Pabst M, Anderle H, Kunert R, Ehrlich HJ, et al. A close look at human IgG sialylation and subclass distribution after lectin fractionation. Proteomics. 2009;9:4143–53.CrossRefPubMed
23.
Barb AW, Brady EK, Prestegard JH. Branch-specific sialylation of IgG-Fc glycans by ST6Gal-I. Biochemistry. 2009;48:9705–7.CrossRefPubMed
24.
Jefferis R. Antibody therapeutics: isotype and glycoform selection. Expert Opin Biol Ther. 2007;7:1401–13.CrossRefPubMed
25.
Arnold JN, Wormald MR, Sim RB, Rudd PM, Dwek RA. The impact of glycosylation on the biological function and structure of human immunoglobulins. Annu Rev Immunol. 2007;25:21–50.CrossRefPubMed
Metadata
Title
Analytical and Functional Aspects of Antibody Sialylation
Authors
Johannes Stadlmann
Martin Pabst
Friedrich Altmann
Publication date
01-05-2010
Publisher
Springer US
Published in
Journal of Clinical Immunology / Issue Special Issue 1/2010
Print ISSN: 0271-9142
Electronic ISSN: 1573-2592
DOI
https://doi.org/10.1007/s10875-010-9409-2

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