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Published in: Archives of Virology 9/2018

01-09-2018 | Annotated Sequence Record

Analysis of the complete genome sequence of a potyvirus from passion fruit suggests its taxonomic classification as a member of a new species

Authors: Ke Yang, Haixia Yan, Li Song, Pengfei Jin, Weiguo Miao, Hongguang Cui

Published in: Archives of Virology | Issue 9/2018

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Abstract

The complete genomic sequence of a telosma mosaic virus (TeMV) isolate (named PasFru), identified in passion fruit in China, was determined. The entire RNA genome of PasFru comprises 10,049 nucleotides (nt) excluding the poly(A) tail and encodes a polyprotein of 3,173 amino acids (aa), flanked by 5’ and 3’ untranslated regions (UTR) of 276 and 251 nt, respectively. Compared with the previous TeMV isolate Hanoi from Telosma cordata, the only documented isolate with the entire genome sequence annotated, PasFru had an extra 87 nt and 89 aa residues at the 3’-end of 5’UTR and the N-terminus of the P1 protein, respectively, which contributed to the genome size difference between PasFru and Hanoi (10,049 nt versus 9,689 nt). Pairwise sequence comparisons showed that PasFru shares 73.6% nt and 80.9% aa sequence identity with the Hanoi isolate at the whole-genome and polyprotein level, respectively, and these values are below the corresponding threshold values for species demarcation in the family Potyviridae. These data suggest that TeMV-PasFru should be classified as a new member of the genus Potyvirus.
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Metadata
Title
Analysis of the complete genome sequence of a potyvirus from passion fruit suggests its taxonomic classification as a member of a new species
Authors
Ke Yang
Haixia Yan
Li Song
Pengfei Jin
Weiguo Miao
Hongguang Cui
Publication date
01-09-2018
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 9/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-018-3885-8

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