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Published in: Archives of Virology 11/2014

01-11-2014 | Brief Report

Analysis of small RNAs derived from Chinese wheat mosaic virus

Authors: Jian Yang, Shi-Ling Zheng, Heng-Mu Zhang, Xiao-Ya Liu, Jing Li, Jun-Min Li, Jian-Ping Chen

Published in: Archives of Virology | Issue 11/2014

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Abstract

The virus-derived small interfering RNAs (vsiRNAs) of Chinese wheat mosaic virus (CWMV), a member of the genus Furovirus, were characterised from wheat plants by deep sequencing. CWMV vsiRNAs of 21–22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CWMV-infected wheat plants. The 5′-terminal base of vsiRNAs was biased towards A/U, suggesting that CWMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were predicted.
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Metadata
Title
Analysis of small RNAs derived from Chinese wheat mosaic virus
Authors
Jian Yang
Shi-Ling Zheng
Heng-Mu Zhang
Xiao-Ya Liu
Jing Li
Jun-Min Li
Jian-Ping Chen
Publication date
01-11-2014
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 11/2014
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-014-2155-7

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